Seamless modification of wild-type induced pluripotent stem cells to the natural CCR5Δ32 mutation confers resistance to HIV infection.

abstract：:The importance of identifying VEGF-independent pathways in pathological angiogenesis is increasingly recognized as a result of the emerging drug resistance to anti-VEGF therapies. PDGF-CC is the third member of the PDGF family discovered after more than two decades of studies on PDGF-AA and PDGF-BB. The biological fun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou X,Kumar A,Lee C,Wang B,Arjunan P,Dong L,Maminishkis A,Tang Z,Li Y,Zhang F,Zhang SZ,Wardega P,Chakrabarty S,Liu B,Wu Z,Colosi P,Fariss RN,Lennartsson J,Nussenblatt R,Gutkind JS,Cao Y,Li X

更新日期：2010-07-06 00:00:00

abstract：:Biologically active (125)I-labeled bovine parathyroid hormone (prepared by electrolytic iodination) and its synthetic NH(2)-terminal (1-34) biologically active fragment bound rapidly and specifically to a purified plasma membrane preparation from bovine renal cortex. Binding of labeled intact hormone or labeled NH(2)-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Bella FP,Dousa TP,Miller SS,Arnaud CD

更新日期：1974-03-01 00:00:00

abstract：:MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences. In contrast, small interfering RNAs (siRNAs), which are derived by processing of long double-stranded RNAs and are o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng Y,Yi R,Cullen BR

更新日期：2003-08-19 00:00:00

abstract：:Humans have altered natural patterns of fire for millennia, but the impact of human-set fires is thought to have been slight in wet closed-canopy forests. In the South Island of New Zealand, Polynesians (Māori), who arrived 700-800 calibrated years (cal y) ago, and then Europeans, who settled ∼150 cal y ago, used fire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McWethy DB,Whitlock C,Wilmshurst JM,McGlone MS,Fromont M,Li X,Dieffenbacher-Krall A,Hobbs WO,Fritz SC,Cook ER

更新日期：2010-12-14 00:00:00

abstract：:The core protein of a small chondroitin/dermatan sulfate proteoglycan expressed by human fibroblasts and present in extracellular matrices in association with collagen has been cloned from a lambda gt11 fibroblast cDNA library. cDNA clones were isolated by use of antibodies specific for the intact proteoglycan and ant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krusius T,Ruoslahti E

更新日期：1986-10-01 00:00:00

abstract：:Cerebral malaria is the most deadly manifestation of infection with Plasmodium falciparum. The pathology of cerebral malaria is characterized by the accumulation of infected erythrocytes (IEs) in the microvasculature of the brain caused by parasite adhesins on the surface of IEs binding to human receptors on microvasc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Claessens A,Adams Y,Ghumra A,Lindergard G,Buchan CC,Andisi C,Bull PC,Mok S,Gupta AP,Wang CW,Turner L,Arman M,Raza A,Bozdech Z,Rowe JA

更新日期：2012-06-26 00:00:00

abstract：:The Wiskott-Aldrich syndrome protein (WASp) plays a major role in coupling T cell antigen receptor (TCR) stimulation to induction of actin cytoskeletal changes required for T cell activation. Here, we report that WASp inducibly binds the sorting nexin 9 (SNX9) in T cells and that WASp, SNX9, p85, and CD28 colocalize w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Badour K,McGavin MK,Zhang J,Freeman S,Vieira C,Filipp D,Julius M,Mills GB,Siminovitch KA

更新日期：2007-01-30 00:00:00

abstract：:The major small nuclear ribonucleoproteins (snRNPs) U1, U2, U4/U6, and U5 share a set of common proteins denoted B/B', D1, D2, D3, E, F, and G which play an important part in the biogenesis of the snRNPs. In addition, there is a link between the common proteins and autoimmunity; the three D proteins, together with B/B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehmeier T,Raker V,Hermann H,Lührmann R

更新日期：1994-12-06 00:00:00

abstract：:The forebrain cholinergic system promotes higher brain function in part by signaling through the M(1) muscarinic acetylcholine receptor (mAChR). During Alzheimer's disease (AD), these cholinergic neurons degenerate, therefore selectively activating M(1) receptors could improve cognitive function in these patients whil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma L,Seager MA,Wittmann M,Jacobson M,Bickel D,Burno M,Jones K,Graufelds VK,Xu G,Pearson M,McCampbell A,Gaspar R,Shughrue P,Danziger A,Regan C,Flick R,Pascarella D,Garson S,Doran S,Kreatsoulas C,Veng L,Lindsley C

更新日期：2009-09-15 00:00:00

abstract：:Platelet-derived growth factor (PDGF) isoforms lead to mitogenic, survival, and chemotactic responses in a variety of mesenchymal cell types during development and in the adult. We have studied the importance of phosphatidylinositol-3' kinase (PI3K) signaling in these responses by mutating the PI3K-binding sites in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heuchel R,Berg A,Tallquist M,Ahlén K,Reed RK,Rubin K,Claesson-Welsh L,Heldin CH,Soriano P

更新日期：1999-09-28 00:00:00

abstract：:Intact squid axons were injected with aequorin and bathed in 3 mM Ca seawater (a concentration close to that of squid blood). Sodium and potassium currents were pharmacologically blocked and repetitive voltage-clamp pulses of a duration of 1.5 ms were applied (to simulate the duration of an action potential) at amplit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mullins LJ,Requena J,Whittembury J

更新日期：1985-03-01 00:00:00

abstract：:High-performance anion-exchange (HPAE) chromatography under alkaline conditions (pH congruent to 13) has been found to efficiently separate neutral oligosaccharides (triose to undecaose) according to molecular size, sugar composition, and linkage of monosaccharide units. The method was able to resolve 1----3, 1----4, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hardy MR,Townsend RR

更新日期：1988-05-01 00:00:00

abstract：:Generating human single-nucleotide polymorphisms (SNPs) is no longer a rate-limiting step for genetic studies of disease. The number of SNPs in public databases already exceeds 200,000, and the total is expected to exceed 1,000,000 within a year. Rather, progress is limited by the inability to genotype large numbers o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirschhorn JN,Sklar P,Lindblad-Toh K,Lim YM,Ruiz-Gutierrez M,Bolk S,Langhorst B,Schaffner S,Winchester E,Lander ES

更新日期：2000-10-24 00:00:00

abstract：:The kappa-selective opioid peptide dynorphin A (DYN) inhibits neuronal adenylate cyclase activity and reduces neuronal voltage-dependent calcium currents. It is not yet known, however, whether the regulation of calcium channel activity is dependent on or independent of the adenylate cyclase/cAMP system. We used the wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross RA,Moises HC,Uhler MD,Macdonald RL

更新日期：1990-09-01 00:00:00

abstract：:Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and solutes are internalized into cells. Macropinocytosis starts with the formation of membrane ruffles at the plasma membrane and ends with their closure. The transient and sequential emergence of phosphoinositides PI(3,4,5)P3 and P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maekawa M,Terasaka S,Mochizuki Y,Kawai K,Ikeda Y,Araki N,Skolnik EY,Taguchi T,Arai H

更新日期：2014-03-18 00:00:00

abstract：:Layer 5 pyramidal neurons process information from multiple cortical layers to provide a major output of cortex. Because of technical limitations it has remained unclear how these cells integrate widespread synaptic inputs located in distantly separated basal and tuft dendrites. Here, we obtained in vivo two-photon ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill DN,Varga Z,Jia H,Sakmann B,Konnerth A

更新日期：2013-08-13 00:00:00

abstract：:We have analyzed ligand-receptor complexes resulting from (i) the incubation of canine hepatic plasma membranes with [125I]iodoglucagon and (ii) subsequent gentle solubilization of receptor-bound ligand with digitonin. The complexes (molecular weight approximately equal to 500,000) retain the radiolabeled ligand durin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mason JC,Tager HS

更新日期：1985-10-01 00:00:00

abstract：:A new method is presented that makes it possible to inject electrons rapidly into redox-active proteins by means of a short light flash. Reduced carboxymethylated cytochrome c (CmCyt c) with carbon monoxide bound to the heme iron is mixed with the oxidized acceptor protein. Upon rapid photodissociation of CO the appar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brzezinski P,Wilson MT

更新日期：1997-06-10 00:00:00

abstract：:In this study, we used functional MRI (fMRI) at high field (3T) to track the time course of activation in the entire basal ganglia circuitry, as well as other motor-related structures, during the explicit learning of a sequence of finger movements over a month of training. Fourteen right-handed healthy volunteers had ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehéricy S,Benali H,Van de Moortele PF,Pélégrini-Issac M,Waechter T,Ugurbil K,Doyon J

更新日期：2005-08-30 00:00:00

abstract：:Biological soil crusts (biocrusts)—communities of mosses, lichens, cyanobacteria, and heterotrophs living at the soil surface—are fundamental components of drylands worldwide, and destruction of biocrusts dramatically alters biogeochemical processes, hydrology, surface energy balance, and vegetation cover. Although th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrenberg S,Reed SC,Belnap J

更新日期：2015-09-29 00:00:00

abstract：:Hair cells of the inner ear, the mechanosensory receptors, convert sound waves into neural signals that are passed to the brain via the auditory nerve. Little is known about the molecular mechanisms that govern the development of hair cell-neuronal connections. We ascertained a family with autosomal recessive deafness...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diaz-Horta O,Abad C,Sennaroglu L,Foster J 2nd,DeSmidt A,Bademci G,Tokgoz-Yilmaz S,Duman D,Cengiz FB,Grati M,Fitoz S,Liu XZ,Farooq A,Imtiaz F,Currall BB,Morton CC,Nishita M,Minami Y,Lu Z,Walz K,Tekin M

更新日期：2016-05-24 00:00:00

abstract：:Aotus monkeys, which are generally killed when infected with the human malaria parasite Plasmodium falciparum, have been identified and grouped by karyotype. These animals were immunized with parasite material obtained from P. falciparum cultivated in vitro which had been maintained in culture for over a year. When su...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reese RT,Trager W,Jensen JB,Miller DA,Tantravahi R

更新日期：1978-11-01 00:00:00

abstract：:The fah1 mutant of Arabidopsis is defective in the accumulation of sinapic acid-derived metabolites, including the guaiacyl-syringyl lignin typical of angiosperms. Earlier results indicated that the FAH1 locus encodes ferulate-5-hydroxylase (F5H), a cytochrome P450-dependent monooxygenase (P450) of the general phenylp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer K,Cusumano JC,Somerville C,Chapple CC

更新日期：1996-07-09 00:00:00

abstract：:Our long-term goal is the in vivo expression of intrinsically colored proteins without the need for further posttranslational modification or chemical functionalization by externally added reagents. Biocompatible (Aza)Indoles (Inds)/(Aza)Tryptophans (Trp) as optical probes represent almost ideal isosteric substitutes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lepthien S,Hoesl MG,Merkel L,Budisa N

更新日期：2008-10-21 00:00:00

abstract：:In virtually all multicellular eukaryotes, mitochondria are transmitted exclusively through one parent, usually the mother. In this short review, we discuss some of the major consequences of uniparental transmission of mitochondria, including deleterious effects in males and selection for increased transmission throug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perlman SJ,Hodson CN,Hamilton PT,Opit GP,Gowen BE

更新日期：2015-08-18 00:00:00

abstract：:In 1950, John Nash contributed a remarkable one-page PNAS article that defined and characterized a notion of equilibrium for n- person games. This notion, now called the "Nash equilibrium," has been widely applied and adapted in economics and other behavioral sciences. Indeed, game theory, with the Nash equilibrium as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holt CA,Roth AE

更新日期：2004-03-23 00:00:00

abstract：:We analyze the effects of an externally applied electric field on thermal fluctuations for a binary electrolyte fluid. We show that the fluctuating Poisson-Nernst-Planck (PNP) equations for charged multispecies diffusion coupled with the fluctuating fluid momentum equation result in enhanced charge transport via a mec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Péraud JP,Nonaka AJ,Bell JB,Donev A,Garcia AL

更新日期：2017-10-10 00:00:00

abstract：:Five DNA fragments have been cloned from yeast, chicken, and mouse DNA that titrate lac repressor in an Escherichia coli lac+ I+Z+ wild-type strain when on a multi-copy plasmid. The five repressor-binding sequences have been identified by DNA sequence determinations and DNase cleavage-inhibition patterns. They share t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simons A,Tils D,von Wilcken-Bergmann B,Müller-Hill B

更新日期：1984-03-01 00:00:00

abstract：:Naive CD4 T cells differentiate into several effector lineages, which generate a stronger and more rapid response to previously encountered immunological challenges. Although effector function is a key feature of adaptive immunity, the molecular basis of this process is poorly understood. Here, we investigated the spa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zorca CE,Kim LK,Kim YJ,Krause MR,Zenklusen D,Spilianakis CG,Flavell RA

更新日期：2015-03-31 00:00:00

abstract：:Rev-Erbβ is a nuclear receptor that couples circadian rhythm, metabolism, and inflammation. Heme binding to the protein modulates its function as a repressor, its stability, its ability to bind other proteins, and its activity in gas sensing. Rev-Erbβ binds Fe3+-heme more tightly than Fe2+-heme, suggesting its activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarkar A,Carter EL,Harland JB,Speelman AL,Lehnert N,Ragsdale SW

更新日期：2021-01-19 00:00:00