MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms.

abstract：:Isolated yeast mitochondria, which synthesize identifiable polypeptides identical to those made in vivo, have been used in an invitro system to study cytoplasmic control of mitochondrial protein synthesis. It has been found that protein synthesis in isolated mitochondria is dependent on an endogenous pool of cytoplasm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poyton RO,Kavanagh J

更新日期：1976-11-01 00:00:00

abstract：:Protein folding poses unique challenges for large, disordered proteins due to the low resolution of structural data accessible in experiment and on the basis of short time scales and limited sampling attainable in computation. Such molecules are uniquely suited to accelerated-sampling molecular dynamics algorithms due...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tarakanova A,Yeo GC,Baldock C,Weiss AS,Buehler MJ

更新日期：2018-07-10 00:00:00

abstract：:The proboscis is an important head appendage in insects that has primarily been thought to process gustatory information during food intake. Indeed, in Drosophila and other insects in which they have been identified, most gustatory receptors are expressed in proboscis neurons. Our previous characterization of the expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwon HW,Lu T,Rützler M,Zwiebel LJ

更新日期：2006-09-05 00:00:00

abstract：:We show that it is possible to direct particles entrained in a fluid along trajectories much like rays of light in classical optics. A microstructured, asymmetric post array forms the core hydrodynamic element and is used as a building block to construct microfluidic metamaterials and to demonstrate refractive, focusi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton KJ,Loutherback K,Inglis DW,Tsui OK,Sturm JC,Chou SY,Austin RH

更新日期：2008-05-27 00:00:00

abstract：:We have determined the volume and location of hippocampal tissue required for normal acquisition of a spatial memory task. Ibotenic acid was used to make bilateral symmetric lesions of 20-100% of hippocampal volume. Even a small transverse block (minislab) of the hippocampus (down to 26% of the total) could support sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moser MB,Moser EI,Forrest E,Andersen P,Morris RG

更新日期：1995-10-10 00:00:00

abstract：:Species- and strain-specific spontaneously occurring tumors have been observed in rodents maintained under normal laboratory conditions. Elucidation of the molecular mechanisms associated with the development of these spontaneous tumors may provide a better understanding of tumor development associated with exposure t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds SH,Stowers SJ,Maronpot RR,Anderson MW,Aaronson SA

更新日期：1986-01-01 00:00:00

abstract：:Complex liquids flow through channels faster than expected, an effect attributed to the formation of low-viscosity depletion layers at the boundaries. Characterization of depletion layer length scale, concentration, and dynamics has remained elusive due in large part to the lack of suitable real-space experimental tec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park SJ,Shakya A,King JT

更新日期：2019-08-13 00:00:00

abstract：:Members of the POU-homeodomain gene family encode transcriptional regulatory molecules that play important roles in terminal differentiation of many organ systems. Sperm-1 (Sprm-1) is a POU domain factor that is exclusively expressed in the differentiating male germ cell. We show here that the Sprm-1 protein is expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearse RV 2nd,Drolet DW,Kalla KA,Hooshmand F,Bermingham JR Jr,Rosenfeld MG

更新日期：1997-07-08 00:00:00

abstract：:The action of calmodulin (CaM) on target proteins is important for a variety of cellular functions. We demonstrate here, however, that the presence of a CaM-binding site on a protein does not necessarily imply a functional effect. The alpha-subunit of the cGMP-gated cation channel of human retinal cones has a CaM-bind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grunwald ME,Zhong H,Lai J,Yau KW

更新日期：1999-11-09 00:00:00

abstract：:Differentiation-inducing factor 1 [DIF-1; 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)-hexan-1-one] induces stalk cell differentiation during Dictyostelium development. It is present as a gradient in the multicellular slug, its lowest concentration being in the anterior. Here we demonstrate the existence of a locali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kay RR,Large S,Traynor D,Nayler O

更新日期：1993-01-15 00:00:00

abstract：:Aquaporin (AQP) 4 is the predominant water channel in the mammalian brain, abundantly expressed in the blood-brain and brain-cerebrospinal fluid interfaces of glial cells. Its function in cerebral water balance has implications in neuropathological disorders, including brain edema, stroke, and head injuries. The 1.8-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho JD,Yeh R,Sandstrom A,Chorny I,Harries WE,Robbins RA,Miercke LJ,Stroud RM

更新日期：2009-05-05 00:00:00

abstract：:Quantitative immunohistochemical analysis of the sphenopalatine ganglion in normal cats revealed that virtually all ganglion cells (98.5%) were immunoreactive to vasoactive intestinal polypeptide (VIP). After surgical removal of this ganglion, the content of both VIP and choline acetyltransferase (acetyl-CoA:choline O...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lundberg JM,Anggård A,Emson P,Fahrenkrug J,Hökfelt T

更新日期：1981-08-01 00:00:00

abstract：:Triose-phosphate isomerase (TPI; D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1) deficiency is a recessive disorder that results in hemolytic anemia and neuromuscular dysfunction. To determine the molecular basis of this disorder, a TPI allele from two unrelated patients homozygous for TPI deficiency was com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daar IO,Artymiuk PJ,Phillips DC,Maquat LE

更新日期：1986-10-01 00:00:00

abstract：:The structure of the NH2-terminal, 20-residue membrane-bound portion of melittin has been computed with empirical energies (ECEPP, Empirical Conformational Energy Program for Peptides). First, a search was made for long stretches of nonpolar residues. Then, the low-energy conformations of these segments were built up ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus MR,Klausner RD,Scheraga HA

更新日期：1982-08-01 00:00:00

abstract：:The paucity of knowledge about cardiomyocyte maturation is a major bottleneck in cardiac regenerative medicine. In development, cardiomyocyte maturation is characterized by orchestrated structural, transcriptional, and functional specializations that occur mainly at the perinatal stage. Sarcomeres are the key cytoskel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo Y,Cao Y,Jardin BD,Sethi I,Ma Q,Moghadaszadeh B,Troiano EC,Mazumdar N,Trembley MA,Small EM,Yuan GC,Beggs AH,Pu WT

更新日期：2021-01-12 00:00:00

abstract：:During cytokinesis, a cleavage furrow generated by actomyosin ring contraction is restructured into the midbody, a platform for the assembly of the abscission machinery that controls the final separation of daughter cells. The polymerization state of F-actin is important during assembly, ingression, disassembly, and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terry SJ,Donà F,Osenberg P,Carlton JG,Eggert US

更新日期：2018-02-27 00:00:00

abstract：:Various enzymatic derivatives of the murine myeloma protein IgA(MOPC-315) were subjected to hapten-binding quantitation and circular dichroic analysis in an attempt to ascertain the structural localization and functional definition of the immunoglobulin active site. The extrinsic Cotton effects observed with near satu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Merz DC,Litman GW,Good RA

更新日期：1974-05-01 00:00:00

abstract：:14-3-3 proteins are ubiquitously expressed regulators of various cellular functions, including proliferation, metabolism, and differentiation, and altered 14-3-3 expression is associated with development and progression of cancer. We report a transforming 14-3-3 oncoprotein, which we identified through conventional cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CH,Ou WB,Mariño-Enriquez A,Zhu M,Mayeda M,Wang Y,Guo X,Brunner AL,Amant F,French CA,West RB,McAlpine JN,Gilks CB,Yaffe MB,Prentice LM,McPherson A,Jones SJ,Marra MA,Shah SP,van de Rijn M,Huntsman DG,Dal Cin P

更新日期：2012-01-17 00:00:00

abstract：:p21(WAF1/Cip1) is one of the best characterized downstream targets of p53, and the growth suppressing function of this cyclin-dependent kinase inhibitor is well established. However, whether p21 exerts a tumor-suppressing function of its own remains to be established. We report here that, similarly to loss of p53, dis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Topley GI,Okuyama R,Gonzales JG,Conti C,Dotto GP

更新日期：1999-08-03 00:00:00

abstract：:Dynamic signals linking the actin cytoskeleton and cell adhesion receptors are essential for morphogenesis during development and normal tissue homeostasis. Abi1 is a central regulator of actin polymerization through interactions with multiple protein complexes. However, the in vivo role of Abi1 remains to be defined....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ring C,Ginsberg MH,Haling J,Pendergast AM

更新日期：2011-01-04 00:00:00

abstract：:Ca(V)1 channels initiate excitation-contraction coupling in skeletal and cardiac muscle. During the fight-or-flight response, epinephrine released by the adrenal medulla and norepinephrine released from sympathetic nerves increase muscle contractility by activation of the β-adrenergic receptor/cAMP-dependent protein k...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Emrick MA,Sadilek M,Konoki K,Catterall WA

更新日期：2010-10-26 00:00:00

abstract：:Human X chromosome DNA was partially purified from a mouse-human hybrid cell line containing a single human chromosome, the X. Enrichment of such DNA was accomplished by two sequential reassociations of radiolabeled hybrid cell DNA with large excesses of mouse DNA. Unreassociated hybrid cell DNA was used as a probe fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmeckpeper BJ,Smith KD,Dorman BP,Ruddle FH,Talbot CC Jr

更新日期：1979-12-01 00:00:00

abstract：:Biodistribution studies of a water-soluble radioactive metallofullerene compound have been conducted using BALB/c mice. To this end, a sample containing Hox@C82 (x = 1, 2) was purified and derivatized to prepare the water-soluble metallofullerol, Hox@C82(OH)y. This metallofullerol was then neutron-activated (165Ho[n,g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cagle DW,Kennel SJ,Mirzadeh S,Alford JM,Wilson LJ

更新日期：1999-04-27 00:00:00

abstract：:Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction of tissue with ATP at low ionic strength and coprecipitation with actin, followed by gel filtration on Sepharose 4B, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barylko B,Wagner MC,Reizes O,Albanesi JP

更新日期：1992-01-15 00:00:00

abstract：:The results of previous preclinical and clinical studies have identified angiogenin (ANG) as a potentially important target for anticancer therapy. Here we report the design and implementation of a high-throughput screening assay to identify small molecules that bind to the ribonucleolytic active site of ANG, which is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kao RY,Jenkins JL,Olson KA,Key ME,Fett JW,Shapiro R

更新日期：2002-07-23 00:00:00

abstract：:Human embryonic zeta-globin chains are alpha-globin-like chains that are normally present during the first three months of gestation. In this investigation, zeta-globin chains measured by a specific and sensitive radioimmunoassay and by an electrophoretic technique were found to be present in all 7 patients studied wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung SW,Wong SC,Clarke BJ,Patterson M,Walker WH,Chui DH

更新日期：1984-10-01 00:00:00

abstract：:AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu W,Kaminski R,Yang F,Zhang Y,Cosentino L,Li F,Luo B,Alvarez-Carbonell D,Garcia-Mesa Y,Karn J,Mo X,Khalili K

更新日期：2014-08-05 00:00:00

abstract：:The ability to regulate gene expression through transcription initiation underlies the adaptability and survival of all bacteria. Recent work has revealed that the transcription machinery in many bacteria diverges from the paradigm that has been established in Escherichia coliMycobacterium tuberculosis (Mtb) encodes t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu DX,Garner AL,Galburt EA,Stallings CL

更新日期：2019-07-02 00:00:00

abstract：:Histone mRNAs are naturally intronless and accumulate efficiently in the cytoplasm. To learn whether there are cis-acting sequences within histone genes that allow efficient cytoplasmic accumulation of RNAs, we made recombinant constructs in which sequences from the mouse H2a gene were cloned into a human beta-globin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang Y,Carmichael GG

更新日期：1997-09-16 00:00:00

abstract：:Gamma delta (γδ) T cells represent a major IL-17 committed T-cell population (γδT17 cells) in the mouse dermis. Following exposure to the inflammatory agent imiquimod (IMQ) the Vγ4(+) subset of γδT cells produce IL-17 in the skin and expand rapidly in draining lymph nodes (LNs). Local IMQ treatment in humans is known ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramírez-Valle F,Gray EE,Cyster JG

更新日期：2015-06-30 00:00:00