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RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro.

Abstract：

:The SOS-inducible ruvA and ruvB gene products of Escherichia coli are required for normal levels of genetic recombination and DNA repair. In vitro, RuvA protein interacts specifically with Holliday junctions and, together with RuvB (an ATPase), promotes their movement along DNA. This process, known as branch migration, is important for the formation of heteroduplex DNA. In this paper, we show that the RuvA and RuvB proteins promote the unwinding of partially duplex DNA. Using single-stranded circular DNA substrates with annealed fragments (52-558 nucleotides in length), we show that RuvA and RuvB promote strand displacement with a 5'-->3' polarity. The reaction is ATP-dependent and its efficiency is inversely related to the length of the duplex DNA. These results show that the ruvA and ruvB genes encode a DNA helicase that specifically recognizes Holliday junctions and promotes branch migration.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Tsaneva IR,Müller B,West SC

doi

10.1073/pnas.90.4.1315

subject

Has Abstract

pub_date

1993-02-15 00:00:00

pages

1315-9

issue

4

eissn

0027-8424

issn

1091-6490

journal_volume

90

pub_type

杂志文章

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