Hypomorphic Notch 3 alleles link Notch signaling to ischemic cerebral small-vessel disease.

abstract：:We present a method for studying multiple retroviral integration events into a small DNA target in vivo. Episomal simian virus 40 (SV40) genomes established by infection of CV-1 cells served as integration targets during subsequent infection with murine leukemia virus (MLV). Using a PCR-based assay for the abundance a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pryciak PM,Müller HP,Varmus HE

更新日期：1992-10-01 00:00:00

abstract：:The quorum sensing signal autoinducer-2 (AI-2) regulates important bacterial behaviors, including biofilm formation and the production of virulence factors. Some bacteria, such as Escherichia coli, can quench the AI-2 signal produced by a variety of species present in the environment, and thus can influence AI-2-depen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marques JC,Oh IK,Ly DC,Lamosa P,Ventura MR,Miller ST,Xavier KB

更新日期：2014-09-30 00:00:00

abstract：:The dominant insulin-stimulated ribosomal protein S6 kinase activity was purified to near homogeneity from insulin-treated 32P-labeled rat H4 hepatoma cells and found to copurify with a 70-kDa 32P-labeled polypeptide. The dominant S6 kinase purified from livers of cycloheximide-treated rats is also a 70-kDa polypeptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Price DJ,Gunsalus JR,Avruch J

更新日期：1990-10-01 00:00:00

abstract：:A cloned Bacillus subtilis gene (sprE) expressed only during the stationary growth phase is shown to encode the subtilisin E protease, an enzyme associated with sporulation. We have determined the DNA sequence of the sprE promoter region and the promoter-proximal half of the structural gene. The sprE gene codes for a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong SL,Price CW,Goldfarb DS,Doi RH

更新日期：1984-02-01 00:00:00

abstract：:Identification of genomic regions that control tissue-specific gene expression is currently problematic. ChIP and high-throughput sequencing (ChIP-seq) of enhancer-associated proteins such as p300 identifies some but not all enhancers active in a tissue. Here we show that co-occupancy of a chromatin region by multiple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He A,Kong SW,Ma Q,Pu WT

更新日期：2011-04-05 00:00:00

abstract：:Gene delivery to the central nervous system is central to the development of gene therapy for neurological diseases. We developed a baculovirus-derived vector, the Bac-CMV-GFP vector, containing a reporter gene encoding for the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. Tw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarkis C,Serguera C,Petres S,Buchet D,Ridet JL,Edelman L,Mallet J

更新日期：2000-12-19 00:00:00

abstract：:In the nitrogen-fixing symbiosis between legume hosts and rhizobia, the bacteria are engulfed by a plant cell membrane to become intracellular organelles. In the model legume Medicago truncatula, internalization and differentiation of Sinorhizobium (also known as Ensifer) meliloti is a prerequisite for nitrogen fixati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim M,Chen Y,Xi J,Waters C,Chen R,Wang D

更新日期：2015-12-08 00:00:00

abstract：:Selective autophagy of damaged mitochondria requires autophagy receptors optineurin (OPTN), NDP52 (CALCOCO2), TAX1BP1, and p62 (SQSTM1) linking ubiquitinated cargo to autophagic membranes. By using quantitative proteomics, we show that Tank-binding kinase 1 (TBK1) phosphorylates all four receptors on several autophagy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richter B,Sliter DA,Herhaus L,Stolz A,Wang C,Beli P,Zaffagnini G,Wild P,Martens S,Wagner SA,Youle RJ,Dikic I

更新日期：2016-04-12 00:00:00

abstract：:Solution at 2.5-A resolution of the three-dimensional structure of H-2Ld with a single nine-residue peptide provides a structural basis for understanding its unique interaction with beta-2 microglobulin (beta2m) and peptide. Consistent with the biological data that show an unusually weak association of Ld with beta2m,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balendiran GK,Solheim JC,Young AC,Hansen TH,Nathenson SG,Sacchettini JC

更新日期：1997-06-24 00:00:00

abstract：:The anti-Tac monoclonal antibody is known to bind to the p55 chain of the human interleukin 2 receptor and to inhibit proliferation of T cells by blocking interleukin 2 binding. However, use of anti-Tac as an immunosuppressant drug would be impaired by the human immune response against this murine antibody. We have th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Queen C,Schneider WP,Selick HE,Payne PW,Landolfi NF,Duncan JF,Avdalovic NM,Levitt M,Junghans RP,Waldmann TA

更新日期：1989-12-01 00:00:00

abstract：:Gilles de la Tourette syndrome (TS) is characterized by tics, which are transiently worsened by stress, acute administration of dopaminergic drugs, and by subtle deficits in motor coordination and sensorimotor gating. It represents the most severe end of a spectrum of tic disorders that, in aggregate, affect ∼ 5% of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu M,Kobets A,Du JC,Lennington J,Li L,Banasr M,Duman RS,Vaccarino FM,DiLeone RJ,Pittenger C

更新日期：2015-01-20 00:00:00

abstract：:Considerable effort has been directed toward controlling tuberculosis, which kills almost two million people yearly. High on the research agenda is the discovery of biomarkers of active tuberculosis (TB) for diagnosis and for monitoring treatment outcome. Rational biomarker discovery requires understanding host-pathog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kunnath-Velayudhan S,Salamon H,Wang HY,Davidow AL,Molina DM,Huynh VT,Cirillo DM,Michel G,Talbot EA,Perkins MD,Felgner PL,Liang X,Gennaro ML

更新日期：2010-08-17 00:00:00

abstract：:A specific high-affinity receptor for leukotriene C4 (LTC4) has been identified on segments of longitudinal smooth muscle from guinea pig ileum, in disrupted cells obtained from the ileal segments, and in subcellular fractions enriched for mitochondrial membranes and plasma membranes, respectively. Specific [3H]LTC4 b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krilis S,Lewis RA,Corey EJ,Austen KF

更新日期：1984-07-01 00:00:00

abstract：:Long-term memory relies on modulation of synaptic connections in response to experience. This plasticity involves trafficking of AMPA receptors (AMPAR) and alteration of spine morphology. Arc, a gene induced by synaptic activity, mediates the endocytosis of AMPA receptors and is required for both long-term and homeost...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peebles CL,Yoo J,Thwin MT,Palop JJ,Noebels JL,Finkbeiner S

更新日期：2010-10-19 00:00:00

abstract：:Epilepsy is characterized by recurrent synchronizations of neuronal activity, which are both a cardinal clinical symptom and a debilitating phenomenon. Although the temporal dynamics of epileptiform synchronizations are well described at the macroscopic level using electrophysiological approaches, less is known about ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldt Muldoon S,Soltesz I,Cossart R

更新日期：2013-02-26 00:00:00

abstract：:Strain OU122, a dnaB mutant of HfrH which stops vegetative DNA synthesis immediately when the temperature is shifted to 43 degrees, was mated at 43 degrees with minicells with and without 1 mM Zn2+. Synthesis of DNA was detected in the mating mixture containing minicells derived from either Fchi925 or F+chi115 cells, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ou JT

更新日期：1975-09-01 00:00:00

abstract：:A renin promoter-large tumor antigen (T antigen) fusion gene was constructed to provide a reporter function for renin expression in transgenic mice. These transgenic mice gave rise to tumors in subcutaneous soft tissue, which was attributed to transgene expression at this site. An immunohistochemical analysis of trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sigmund CD,Jones CA,Mullins JJ,Kim U,Gross KW

更新日期：1990-10-01 00:00:00

abstract：:Eukaryote cell division features a chromosome compaction-decompaction cycle that is synchronized with their physical and topological segregation. It has been proposed that lengthwise compaction of chromatin into mitotic chromosomes via loop extrusion underlies the compaction-segregation/resolution process. We analyze ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brahmachari S,Marko JF

更新日期：2019-12-10 00:00:00

abstract：:Given the widely noted increase in the warming effects of rising greenhouse gas concentrations, it has been unclear why global surface temperatures did not rise between 1998 and 2008. We find that this hiatus in warming coincides with a period of little increase in the sum of anthropogenic and natural forcings. Declin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann RK,Kauppi H,Mann ML,Stock JH

更新日期：2011-07-19 00:00:00

abstract：:Benzophenones are among the most useful photocrosslinking agents in biology. We have evolved an orthogonal aminoacyl-tRNA synthetase/tRNA pair that makes possible the in vivo incorporation of p-benzoyl-l-phenylalanine into proteins in Escherichia coli in response to the amber codon, TAG. This unnatural amino acid was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chin JW,Martin AB,King DS,Wang L,Schultz PG

更新日期：2002-08-20 00:00:00

abstract：:Modeling studies of terrestrial extrasolar planetary climates are now including the effects of ocean circulation due to a recognition of the importance of oceans for climate; indeed, the peak equator-pole ocean heat transport on Earth peaks at almost half that of the atmosphere. However, such studies have made the ass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cullum J,Stevens DP,Joshi MM

更新日期：2016-04-19 00:00:00

abstract：:A fundamental question in the study of human language acquisition centers around apportioning explanatory force between the experience of the learner and the core knowledge that allows learners to represent that experience. We provide a previously unidentified kind of data identifying children's contribution to langua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han CH,Musolino J,Lidz J

更新日期：2016-01-26 00:00:00

abstract：:Primary prostate cancer is the most common malignancy in men but has highly variable outcomes, highlighting the need for biomarkers to determine which patients can be managed conservatively. Few large prostate oncogenome resources currently exist that combine the molecular and clinical outcome data necessary to discov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hieronymus H,Schultz N,Gopalan A,Carver BS,Chang MT,Xiao Y,Heguy A,Huberman K,Bernstein M,Assel M,Murali R,Vickers A,Scardino PT,Sander C,Reuter V,Taylor BS,Sawyers CL

更新日期：2014-07-29 00:00:00

abstract：:Two major problems have to be solved in studies of genes near breakpoints of chromosome abnormalities and in large-scale genomic mapping projects: (i) the identification of genes within the large amount on nontranscribed DNA and (ii) the determination of the tissues in which the identified genes are transcribed. In si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boehm T,Gonzalez-Sarmiento R,Kennedy M,Rabbitts TH

更新日期：1991-05-01 00:00:00

abstract：:Glycogenin initiates the synthesis of a maltosaccharide chain covalently attached to itself on Tyr195 via a stepwise glucosylation reaction, priming glycogen synthesis. We have captured crystallographic snapshots of human glycogenin during its reaction cycle, revealing a dynamic conformational switch between ground an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaikuad A,Froese DS,Berridge G,von Delft F,Oppermann U,Yue WW

更新日期：2011-12-27 00:00:00

abstract：:The mutagenic effect of low linear energy transfer ionizing radiation is reduced for a given dose as the dose rate (DR) is reduced to a low level, a phenomenon known as the direct DR effect. Our reanalysis of published data shows that for both somatic and germ-line mutations there is an opposite, inverse DR effect, wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vilenchik MM,Knudson AG Jr

更新日期：2000-05-09 00:00:00

abstract：:We test the conjecture that becoming unemployed erodes the extent to which a person acknowledges earned entitlement. We use behavioral experiments to generate incentive-compatible measures of individuals' tendencies to acknowledge earned entitlement and incorporate these experiments in a two-stage study. In the first ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barr A,Miller L,Ubeda P

更新日期：2016-04-26 00:00:00

abstract：:The E1A gene of adenovirus type 5 encodes two major proteins of 289 and 243 amino acid residues, which are identical except that the larger protein has an internal stretch of 46 amino acids required for efficient trans-activation of early viral promoters. This domain contains a consensus zinc finger motif (Cys-Xaa2-Cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Culp JS,Webster LC,Friedman DJ,Smith CL,Huang WJ,Wu FY,Rosenberg M,Ricciardi RP

更新日期：1988-09-01 00:00:00

abstract：:Pure cultures of human breast epithelial cells and of fibroblastic cells in early passage provided the opportunity to ask whether either cell type had the capability for metabolizing chemical carcinogens and, if so, was the fate of the metabolic products compatible with chemical carcinogens being a factor in the initi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stampfer MR,Bartholomew JC,Smith HS,Bartley JC

更新日期：1981-10-01 00:00:00

abstract：:Prostaglandin E1 and the beta-adrenergic hormone l-isoproterenol stimulated cyclic AMP formation in both nucleated and enucleated myeloid leukemic cells that could be induced to differentiate normally to mature cells by the macrophage- and granulocyte-inducing protein MGI (MGI+D+ cells). Enucleated as well as nucleate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simantov R,Shkolnik T,Sachs L

更新日期：1980-08-01 00:00:00