Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems.

abstract：:Posttranscriptional RNA regulation is important in determining the plasticity of cellular phenotypes. However, mechanisms of how RNA binding proteins (RBPs) influence cellular behavior are poorly understood. We show here that the RBP embryonic lethal abnormal vision like 1 (ELAVL1, also know as HuR) regulates the alte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang SH,Elemento O,Zhang J,Zhuang ZW,Simons M,Hla T

更新日期：2014-12-23 00:00:00

abstract：:The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)H]4-(N-maleimido)-benzyltrimethylammon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin A,Cowburn D

更新日期：1973-12-01 00:00:00

abstract：:We investigate the problem of learning to play the game of rock-paper-scissors. Each player attempts to improve her/his average score by adjusting the frequency of the three possible responses, using reinforcement learning. For the zero sum game the learning process displays Hamiltonian chaos. Thus, the learning traje...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sato Y,Akiyama E,Farmer JD

更新日期：2002-04-02 00:00:00

abstract：:Metazoan arrestins bind to seven-transmembrane (7TM) receptors to regulate function. Aspergillus nidulans PalF, a protein involved in the fungal ambient pH signaling pathway, contains arrestin N-terminal and C-terminal domains and binds strongly to two different regions within the C-terminal cytoplasmic tail of the 7T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herranz S,Rodríguez JM,Bussink HJ,Sánchez-Ferrero JC,Arst HN Jr,Peñalva MA,Vincent O

更新日期：2005-08-23 00:00:00

abstract：:Sugar transport across tonoplasts is essential for maintaining cellular sugar homeostasis and metabolic balance in plant cells. It remains unclear, however, how this process is regulated among different classes of sugar transporters. Here, we identified a tonoplast H+/glucose symporter, MdERDL6-1, from apples, which w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Li B,Wu L,Li H,Wang Z,Wei X,Ma B,Zhang Y,Ma F,Ruan YL,Li M

更新日期：2021-01-05 00:00:00

abstract：:Sixteen hybridomas have been identified that secrete antibodies specific to horizontal cells in the carp retina. The hybridomas have been classified into three groups based on their antibody staining patterns: group I, staining associated with all horizontal cells; group II, staining associated with the most abundant ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Young LH,Dowling JE

更新日期：1984-10-01 00:00:00

abstract：:The temperature coefficient of equilibrium isotope fractionation in the heavy elements is shown to be larger at high temperatures than that expected from the well-studied vibrational isotope effects. The difference in the isotopic behavior of the heavy elements as compared with the light elements is due to the large n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bigeleisen J

更新日期：1996-09-03 00:00:00

abstract：:Ab initio and density functional calculations have been carried out to more fully understand the factors controlling the catalytic activity of the Thermus aquaticus DNA methyltransferase (MTaqI) in the N-methylation at the N(6) of an adenine nucleobase. The noncatalyzed reaction was modeled as a methyl transfer from t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newby ZE,Lau EY,Bruice TC

更新日期：2002-06-11 00:00:00

abstract：:The appearance of jaws was a turning point in vertebrate evolution because it allowed primitive vertebrates to capture and process large, motile prey. The vertebrate jaw consists of separate dorsal and ventral skeletal elements connected by a joint. How this structure evolved from the unjointed gill bar of a jawless a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cerny R,Cattell M,Sauka-Spengler T,Bronner-Fraser M,Yu F,Medeiros DM

更新日期：2010-10-05 00:00:00

abstract：:Major histocompatibility complex (MHC) class I multimer technology has become an indispensable immunological assay system to dissect antigen-specific cytotoxic CD8(+) T cell responses by flow cytometry. However, the development of high-throughput assay systems, in which T cell responses against a multitude of epitopes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bakker AH,Hoppes R,Linnemann C,Toebes M,Rodenko B,Berkers CR,Hadrup SR,van Esch WJ,Heemskerk MH,Ovaa H,Schumacher TN

更新日期：2008-03-11 00:00:00

abstract：:RNA interference, mediated by small interfering RNAs (siRNAs), is a conserved regulatory process that has evolved as an antiviral defense mechanism in plants and animals. It is not known whether host cells also use siRNAs as an antibacterial defense mechanism in eukaryotes. Here, we report the discovery of an endogeno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katiyar-Agarwal S,Morgan R,Dahlbeck D,Borsani O,Villegas A Jr,Zhu JK,Staskawicz BJ,Jin H

更新日期：2006-11-21 00:00:00

abstract：:Cell adhesion molecules (CAMs) may act as regulators of morphogenesis by constraining cell motion, forming borders, and controlling intercellular communications that lead to embryonic induction. This postulated causal role of CAMs in inductive events was tested here in an in vitro system of feather induction. In the d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallin WJ,Chuong CM,Finkel LH,Edelman GM

更新日期：1986-11-01 00:00:00

abstract：:Effects of opiate receptor antagonists on centrally mediated cardiovascular responses to clonidine and beta-endorphin were studied in urethane-anesthetized spontaneously hypertensive Okamoto-Aoki rats (SHR), normotensive Sprague-Dawley rats, and Sprague-Dawley rats made hypertensive with deoxycorticosterone pivalate/s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mosqueda-Garcia R,Kunos G

更新日期：1987-12-01 00:00:00

abstract：:The Mediator complex forms the bridge between transcriptional activators and RNA polymerase II. Mediator subunit Med1/TRAP220 is a key component of Mediator originally found to associate with nuclear hormone receptors. Med1 deficiency causes lethality at embryonic day 11.5 because of defects in heart and placenta deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stumpf M,Waskow C,Krötschel M,van Essen D,Rodriguez P,Zhang X,Guyot B,Roeder RG,Borggrefe T

更新日期：2006-12-05 00:00:00

abstract：:RNase T2 enzymes are conserved in most eukaryotic genomes, and expression patterns and phylogenetic analyses suggest that they may carry out an important housekeeping role. However, the nature of this role has been elusive. Here we show that RNS2, an intracellular RNase T2 from Arabidopsis thaliana, is essential for n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hillwig MS,Contento AL,Meyer A,Ebany D,Bassham DC,Macintosh GC

更新日期：2011-01-18 00:00:00

abstract：:Eph receptor tyrosine kinases and their ephrin ligands regulate cell navigation during normal and oncogenic development. Signaling of Ephs is initiated in a multistep process leading to the assembly of higher-order signaling clusters that set off bidirectional signaling in interacting cells. However, the structural an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Himanen JP,Yermekbayeva L,Janes PW,Walker JR,Xu K,Atapattu L,Rajashankar KR,Mensinga A,Lackmann M,Nikolov DB,Dhe-Paganon S

更新日期：2010-06-15 00:00:00

abstract：:Allostatic load (AL) is a measure of overall physiological wear-and-tear over the life course, which could partially be the consequence of early life exposures. AL could allow a better understanding of the potential biological pathways playing a role in the construction of the social gradient in adult health. To explo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barboza Solís C,Kelly-Irving M,Fantin R,Darnaudéry M,Torrisani J,Lang T,Delpierre C

更新日期：2015-02-17 00:00:00

abstract：:Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) genes encode transcription factors belonging to the orphan subfamily of the steroid/thyroid hormone receptor superfamily. Two COUP-TF counterparts have been cloned from mouse. In an attempt to study the function of these genes in the developing central...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiu Y,Cooney AJ,Kuratani S,DeMayo FJ,Tsai SY,Tsai MJ

更新日期：1994-05-10 00:00:00

abstract：:Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme. This inhibition of enzyme activity by rifamycin SV derivatives is due to a r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu AM,Ting RC,Gallo RC

更新日期：1973-05-01 00:00:00

abstract：:The lectin chaperone calreticulin (CRT) assists the folding and quality control of newly synthesized glycoproteins in the endoplasmic reticulum (ER). It interacts with ERp57, a thiol-disulfide oxidoreductase that promotes the formation of disulfide bonds in glycoproteins bound by CRT. Here, we investigated the interac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frickel EM,Riek R,Jelesarov I,Helenius A,Wuthrich K,Ellgaard L

更新日期：2002-02-19 00:00:00

abstract：:We have obtained the electron paramagnetic resonance spectrum of Cu2+ bound in a tetragonal single crystal of hen egg-white lysozyme. A part of this spectrum has been shown to originate from Cu2+ ions bound at the site designated as B by Teichberg et al. [Teichberg, V.I., Sharon, N., Moult, J., Smilansky, A. & Yonath,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hutchison CA Jr,Singel DJ,Hadad MN,Kemple MD

更新日期：1980-09-01 00:00:00

abstract：:The nuclear receptor REV-ERBα integrates the circadian clock with hepatic glucose and lipid metabolism by nucleating transcriptional comodulators at genomic regulatory regions. An interactomic approach identified O-GlcNAc transferase (OGT) as a REV-ERBα-interacting protein. By shielding cytoplasmic OGT from proteasoma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berthier A,Vinod M,Porez G,Steenackers A,Alexandre J,Yamakawa N,Gheeraert C,Ploton M,Maréchal X,Dubois-Chevalier J,Hovasse A,Schaeffer-Reiss C,Cianférani S,Rolando C,Bray F,Duez H,Eeckhoute J,Lefebvre T,Staels B,Lef

更新日期：2018-11-20 00:00:00

abstract：:As the Copenhagen Accord indicates, most of the international community agrees that global mean temperature should not be allowed to rise more than two degrees Celsius above preindustrial levels to avoid unacceptable damages from climate change. The scientific evidence distilled in the Fourth Assessment Report of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heitzig J,Lessmann K,Zou Y

更新日期：2011-09-20 00:00:00

abstract：:Double-positive (DP) thymocytes respond to intrathymic T-cell receptor (TCR) signals by undergoing positive selection and lineage differentiation into single-positive (SP) mature cells. Concomitant with these well-characterized events is the acquisition of a mature T-cell gene expression program characterized by the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi LZ,Saravia J,Zeng H,Kalupahana NS,Guy CS,Neale G,Chi H

更新日期：2017-01-03 00:00:00

abstract：:The abundances of (92)Nb and (146)Sm in the early solar system are determined from meteoritic analysis, and their stellar production is attributed to the p process. We investigate if their origin from thermonuclear supernovae deriving from the explosion of white dwarfs with mass above the Chandrasekhar limit is in agr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lugaro M,Pignatari M,Ott U,Zuber K,Travaglio C,Gyürky G,Fülöp Z

更新日期：2016-01-26 00:00:00

abstract：:Carcinogenic 9-anthryloxirane binds covalently to calf thymus DNA and poly(dA-dT). Application of the technique for DNA sequence determination shows that acid cleavage of the modified DNA frees approximately half of the anthryl groups from the DNA. HPLC analysis indicates that an adenine adduct and the glycol derived ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang NC,Chang CW

更新日期：1985-08-01 00:00:00

abstract：:A specific antibody against proinsulin has been obtained by adsorbing the original anti-proinsulin guinea pig serum with a solid immunosorbent of Sephadex-insulin. The specificity of the antibody against antigenic determinants of proinsulin was established by radioimmunoassay and by passive cutaneous anaphylaxis (PCA)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yip CC,Logothetopoulos J

更新日期：1969-02-01 00:00:00

abstract：:Osmotically shocked Escherichia coli and membrane vesicle ghosts from E. coli cells have lost the ability to accumulate potassium by active transport. The addition of valinomycin to the membrane ghosts restores the capacity to accumulate radioactive (42)K and (86)Rb by a temperature- and energy-dependent process. Memb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhattacharyya P,Epstein W,Silver S

更新日期：1971-07-01 00:00:00

abstract：:Shift work is a risk factor for hypertension, inflammation, and cardiovascular disease. This increased risk cannot be fully explained by classic risk factors. One of the key features of shift workers is that their behavioral and environmental cycles are typically misaligned relative to their endogenous circadian syste...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morris CJ,Purvis TE,Hu K,Scheer FA

更新日期：2016-03-08 00:00:00

abstract：:Although it is well described in model membranes, little is known about phase separation in biological membranes. Here, we provide evidence for a coexistence of at least two different lipid bilayer phases in the apical plasma membrane of epithelial cells. Phase connectivity was assessed by measuring long-range diffusi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meder D,Moreno MJ,Verkade P,Vaz WL,Simons K

更新日期：2006-01-10 00:00:00