The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins.

abstract：:Previous work showed that cultured human pancreatic cancer cells overexpress the epidermal growth factor (EGF) receptor. In the present study, we sought to determine whether some of these cell lines produce transforming growth factor alpha (TGF-alpha). Utilizing a radiolabeled TGF-alpha cDNA in hybridization experimen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JJ,Derynck R,Korc M

更新日期：1987-11-01 00:00:00

abstract：:Paired immunoglobulin-like type 2 receptor α (PILRα) and β (PILRβ) belong to the PILR family and are related to innate immune regulation in various species. Despite their high sequence identity, PILRα and PILRβ are shown to have variant sialic acid (SA) binding avidities. To explore the molecular basis of this interac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Q,Lu G,Qi J,Wang H,Xuan Y,Wang Q,Li Y,Zhang Y,Zheng C,Fan Z,Yan J,Gao GF

更新日期：2014-06-03 00:00:00

abstract：:Several yeast activators are phosphorylated by SRB10, a cyclin-dependent kinase associated with the transcriptional machinery. Sites of phosphorylation are found outside the activating region in each case, and the modification has different physiological consequences in different cases. We show here that certain acidi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ansari AZ,Koh SS,Zaman Z,Bongards C,Lehming N,Young RA,Ptashne M

更新日期：2002-11-12 00:00:00

abstract：:Optogenetic methods have been highly effective for suppressing neural activity and modulating behavior in rodents, but effects have been much smaller in primates, which have much larger brains. Here, we present a suite of technologies to use optogenetics effectively in primates and apply these tools to a classic quest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acker L,Pino EN,Boyden ES,Desimone R

更新日期：2016-11-15 00:00:00

abstract：:The immunocytochemical demonstration of (pro)insulin on intracellular membrane compartments of the pancreatic B cell reveals that the immunolabeling detected by the protein A/gold method is associated, at the level of the Golgi apparatus, with the inner aspect of the cisternal membranes; on the secretory granules, by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orci L,Ravazzola M,Perrelet A

更新日期：1984-11-01 00:00:00

abstract：:Dynamic signals linking the actin cytoskeleton and cell adhesion receptors are essential for morphogenesis during development and normal tissue homeostasis. Abi1 is a central regulator of actin polymerization through interactions with multiple protein complexes. However, the in vivo role of Abi1 remains to be defined....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ring C,Ginsberg MH,Haling J,Pendergast AM

更新日期：2011-01-04 00:00:00

abstract：:Previous attempts to express functional DNA cytosine methyltransferase (EC 2.1.1.37) in cells transfected with the available Dnmt cDNAs have met with little or no success. We show that the published Dnmt sequence encodes an amino terminal-truncated protein that is tolerated only at very low levels when stably expresse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tucker KL,Talbot D,Lee MA,Leonhardt H,Jaenisch R

更新日期：1996-11-12 00:00:00

abstract：:Mitochondria isolated from maize plants with S-type male-sterile cytoplasms are capable of synthesizing four species of RNA at concentrations of actinomycin D that eliminate all DNA-directed RNA synthesis. No RNA synthesis occurs under the same conditions with mitochondria from plants possessing normal (N) cytoplasm o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finnegan PM,Brown GG

更新日期：1986-07-01 00:00:00

abstract：:We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNF1) and 87,093 (pNF2) bp with average G+C ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishikawa J,Yamashita A,Mikami Y,Hoshino Y,Kurita H,Hotta K,Shiba T,Hattori M

更新日期：2004-10-12 00:00:00

abstract：:Our recent demonstration that many eukaryotic mRNAs contain sequences complementary to rRNA led to the hypothesis that these sequences might mediate specific interactions between mRNAs and ribosomes and thereby affect translation. In the present experiments, the ability of complementary sequences to bind to rRNA was i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tranque P,Hu MC,Edelman GM,Mauro VP

更新日期：1998-10-13 00:00:00

abstract：:The translational apparatus in cell-free extracts prepared from L cells infected with reovirus undergoes a time-dependent transition from cap dependence to cap independence. Extracts from uninfected L cells translate capped reovirus mRNA at high efficiency and synthesize the expected three size classes of reovirus pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skup D,Millward S

更新日期：1980-01-01 00:00:00

abstract：:Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armache JP,Jarasch A,Anger AM,Villa E,Becker T,Bhushan S,Jossinet F,Habeck M,Dindar G,Franckenberg S,Marquez V,Mielke T,Thomm M,Berninghausen O,Beatrix B,Söding J,Westhof E,Wilson DN,Beckmann R

更新日期：2010-11-16 00:00:00

abstract：:Using T- and B-cell deficient C.B-17 mice with the scid mutation, we have previously documented the existence of a T-cell-independent but interferon gamma-dependent pathway of macrophage activation that confers upon the host partial resistance to the facultative intracellular bacterium Listeria monocytogenes. This pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rogers HW,Sheehan KC,Brunt LM,Dower SK,Unanue ER,Schreiber RD

更新日期：1992-02-01 00:00:00

abstract：:Voltage-gated Na(+) channels are composed of pore-forming alpha and auxiliary beta subunits. The majority of Na(+) channels in the heart contain tetrodotoxin (TTX)-insensitive Na(v)1.5 alpha subunits, but TTX-sensitive brain-type Na(+) channel alpha subunits are present and functionally important in the transverse tub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maier SK,Westenbroek RE,Yamanushi TT,Dobrzynski H,Boyett MR,Catterall WA,Scheuer T

更新日期：2003-03-18 00:00:00

abstract：:Many Drosophila non-long terminal repeat (LTR) retrotransposons actively transpose into internal, gene-rich regions of chromosomes but do not transpose onto chromosome ends. HeT-A and TART are remarkable exceptions; they form telomeres of Drosophila by repeated transpositions onto the ends of chromosomes and never tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rashkova S,Karam SE,Pardue ML

更新日期：2002-03-19 00:00:00

abstract：:Purified inside-out vesicles from human erythrocytes were used to investigate the active transport of oxidized glutathione (GSSG). Incubation of vesicles and GSSG in the presence of ATP resulted in the transport of GSSG into the vesicles. When vesicles were incubated with reduced glutathione (GSH), no transport was ob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo T,Dale GL,Beutler E

更新日期：1980-11-01 00:00:00

abstract：:Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-A crystal structure of the physiological complex of the large ribosomal s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baram D,Pyetan E,Sittner A,Auerbach-Nevo T,Bashan A,Yonath A

更新日期：2005-08-23 00:00:00

abstract：:Plasmid pT181 is a 4437-base-pair, multicopy plasmid of Staphylococcus aureus that encodes tetracycline resistance. The replication of the leading strand of pT181 DNA initiates by covalent extension of a site-specific nick generated by the initiator protein at the origin of replication and proceeds by an asymmetric ro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birch P,Khan SA

更新日期：1992-01-01 00:00:00

abstract：:Bone marrow mononuclear cells (BMC) from patients with ischemic cardiomyopathy (ICMP) show a reduced neovascularization capacity in vivo. NO plays an important role in neovascularization, and NO bioavailability is typically reduced in patients with ICMP. We investigated whether the impaired neovascularization capacity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasaki K,Heeschen C,Aicher A,Ziebart T,Honold J,Urbich C,Rossig L,Koehl U,Koyanagi M,Mohamed A,Brandes RP,Martin H,Zeiher AM,Dimmeler S

更新日期：2006-09-26 00:00:00

abstract：:Ovarian cancer remains the most lethal gynecologic malignancy. We analyzed the mutational landscape of 64 primary, 41 metastatic, and 17 recurrent fresh-frozen tumors from 77 patients along with matched normal DNA, by whole-exome sequencing (WES). We also sequenced 13 pairs of synchronous bilateral ovarian cancer (SBO...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li C,Bonazzoli E,Bellone S,Choi J,Dong W,Menderes G,Altwerger G,Han C,Manzano A,Bianchi A,Pettinella F,Manara P,Lopez S,Yadav G,Riccio F,Zammataro L,Zeybek B,Yang-Hartwich Y,Buza N,Hui P,Wong S,Ravaggi A,Bigno

更新日期：2019-01-08 00:00:00

abstract：:Four monoclonal anti-parathyroid antibodies were generated after immunization of mice with intact cells from human parathyroid tissue. All four monoclonal antibodies reacted in immunohistochemistry with structures present on parathyroid epithelial cells and proximal-tubule cells of the kidney but were unreactive with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Juhlin C,Holmdahl R,Johansson H,Rastad J,Akerström G,Klareskog L

更新日期：1987-05-01 00:00:00

abstract：:The sea anemone, Nematostella vectensis, has become an attractive new model organism for comparative genomics and evolutionary developmental biology. Over the last few years, many genes have been isolated and their expression patterns studied to gain insight into their function. More recently, functional tools have be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renfer E,Amon-Hassenzahl A,Steinmetz PR,Technau U

更新日期：2010-01-05 00:00:00

abstract：:The emergence of bacterial multidrug resistance to antibiotics threatens to cause regression to the preantibiotic era. Here we present the crystal structure of the large ribosomal subunit from Staphylococcus aureus, a versatile Gram-positive aggressive pathogen, and its complexes with the known antibiotics linezolid a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyal Z,Matzov D,Krupkin M,Wekselman I,Paukner S,Zimmerman E,Rozenberg H,Bashan A,Yonath A

更新日期：2015-10-27 00:00:00

abstract：:Variable regions of Ig chains provide the antigen recognition portion of B-cell receptors and derivative antibodies. Ig heavy-chain variable region exons are assembled developmentally from V, D, J gene segments. Each variable region contains three antigen-contacting complementarity-determining regions (CDRs), with CDR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang JK,Wang C,Du Z,Meyers RM,Kepler TB,Neuberg D,Kwong PD,Mascola JR,Joyce MG,Bonsignori M,Haynes BF,Yeap LS,Alt FW

更新日期：2017-08-08 00:00:00

abstract：:The eukaryote endomembrane system contains a class of H(+)-pumping ATPase (H(+)-ATPases) of the vacuolar type (V-ATPases) that are responsible for the acidification of organelles. Their action is critical to numerous physiological processes, but the regulatory mechanisms that may control activity are not yet fully und...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davies JM,Hunt I,Sanders D

更新日期：1994-08-30 00:00:00

abstract：:Dematerialization, represented by declining consumption per GDP of energy or of goods, offers some hope for rising environmental quality with development. The declining proportion of income spent on staples as affluence grows, which income elasticity <1.0 measures, makes dematerialization widespread. Further, as learn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ausubel JH,Waggoner PE

更新日期：2008-09-02 00:00:00

abstract：:Hearing loss is the main limitation of radiation therapy for vestibular schwannoma (VS), and identifying treatment options that minimize hearing loss are urgently needed. Treatment with bevacizumab is associated with tumor control and hearing improvement in neurofibromatosis type 2 (NF2) patients; however, its effect ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao X,Zhao Y,Stemmer-Rachamimov AO,Liu H,Huang P,Chin S,Selig MK,Plotkin SR,Jain RK,Xu L

更新日期：2015-11-24 00:00:00

abstract：:Mass spectrometry (MS)-based quantitative interaction proteomics has successfully elucidated specific protein-protein, DNA-protein, and small molecule-protein interactions. Here, we developed a gel-free, sensitive, and scalable technology that addresses the important area of RNA-protein interactions. Using aptamer-tag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butter F,Scheibe M,Mörl M,Mann M

更新日期：2009-06-30 00:00:00

abstract：:We present a method for studying RNA processing and ribonucleoprotein assembly in vivo, by using RNA synthesized in vitro. SP6-transcribed 32P-labeled U2 small nuclear RNA precursor molecules were introduced into cultured human 293 cells by calcium phosphate-mediated uptake, as in standard DNA transfection experiments...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kleinschmidt AM,Pederson T

更新日期：1990-02-01 00:00:00

abstract：:The interaction of human high density lipoproteins (HDL) with isolated human monocytes from control and Tangier patients was studied in tissue culture experiments. It was observed that normal monocytes, similar to mouse peritoneal macrophages, bind HDL to a cell surface receptor, internalize the bound HDL particles, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmitz G,Assmann G,Robenek H,Brennhausen B

更新日期：1985-09-01 00:00:00