Replication of single-stranded plasmid pT181 DNA in vitro.

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent endogenous activator of the cell death pathway and functions by activating the cell surface death receptors 4 and 5 (DR4 and DR5). TRAIL is nontoxic in vivo and preferentially kills neoplastically transformed cells over normal cells by an unde...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chinnaiyan AM,Prasad U,Shankar S,Hamstra DA,Shanaiah M,Chenevert TL,Ross BD,Rehemtulla A

更新日期：2000-02-15 00:00:00

abstract：:We have constructed and cloned, in bacteria, recombinant plasmids containing DNA complementary to mRNA coding for a pancreatic pre-prosomatostatin, a product of the cell-free translation of pancreatic islet mRNAs shown previously by immunoprecipitation to be a precursor of somatostatin. A clone containing a nearly ful...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodman RH,Jacobs JW,Chin WW,Lund PK,Dee PC,Habener JF

更新日期：1980-10-01 00:00:00

abstract：:Photoreceptors of the Xenopus laevis retina are the site of a circadian clock. As part of a differential display screen for rhythmic gene products in this system, we have identified a photoreceptor-specific mRNA expressed in peak abundance at night. cDNA cloning revealed an open reading frame encoding a putative 388 a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green CB,Besharse JC

更新日期：1996-12-10 00:00:00

abstract：:We performed combinatorial peptide library screening in vivo on a novel human prostate cancer xenograft that is androgen-independent and induces a robust osteoblastic reaction in bonelike matrix and soft tissue. We found two peptides, PKRGFQD and SNTRVAP, which were enriched in the tumors, targeted the cell surface of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandelin J,Cardó-Vila M,Driessen WH,Mathew P,Navone NM,Lin SH,Logothetis CJ,Rietz AC,Dobroff AS,Proneth B,Sidman RL,Pasqualini R,Arap W

更新日期：2015-03-24 00:00:00

abstract：:Gel-forming mucins, the primary macromolecular components of airway mucus, facilitate airway clearance by mucociliary transport. In cystic fibrosis (CF) altered mucus properties impair mucociliary transport. Airways primarily secrete two closely related gel-forming mucins, MUC5B and MUC5AC. However, their morphologic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ostedgaard LS,Moninger TO,McMenimen JD,Sawin NM,Parker CP,Thornell IM,Powers LS,Gansemer ND,Bouzek DC,Cook DP,Meyerholz DK,Abou Alaiwa MH,Stoltz DA,Welsh MJ

更新日期：2017-06-27 00:00:00

abstract：:Protein-based hydrogels have emerged as promising alternatives to synthetic hydrogels for biomedical applications, owing to the precise control of structure and function enabled by protein engineering. Nevertheless, strategies for assembling 3D molecular networks that carry the biological information encoded in full-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun F,Zhang WB,Mahdavi A,Arnold FH,Tirrell DA

更新日期：2014-08-05 00:00:00

abstract：:Strongylocentrotus intermedius (A. Agassiz, 1863) is an economically important sea urchin inhabiting the northwest Pacific region of Asia. The northern Primorye (Sea of Japan) populations of S. intermedius consist of two sympatric morphological forms, "usual" (U) and "gray" (G). The two forms are significantly differe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balakirev ES,Pavlyuchkov VA,Ayala FJ

更新日期：2008-10-21 00:00:00

abstract：:The connexin 43 (Cx43) hemichannel (HC) in the mechanosensory osteocytes is a major portal for the release of factors responsible for the anabolic effects of mechanical loading on bone formation and remodeling. However, little is known about how the Cx43 molecule responds to mechanical stimulation leading to the openi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Batra N,Burra S,Siller-Jackson AJ,Gu S,Xia X,Weber GF,DeSimone D,Bonewald LF,Lafer EM,Sprague E,Schwartz MA,Jiang JX

更新日期：2012-02-28 00:00:00

abstract：:TGF-beta-activated kinase-1 (TAK1), also known as MAPKK kinase-7 (MAP3K7), is a candidate effector of multiple circuits in cardiac biology and disease. Here, we show that inhibition of TAK1 in mice by a cardiac-specific dominant-negative mutation evokes electrophysiological and biochemical properties reminiscent of hu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie M,Zhang D,Dyck JR,Li Y,Zhang H,Morishima M,Mann DL,Taffet GE,Baldini A,Khoury DS,Schneider MD

更新日期：2006-11-14 00:00:00

abstract：:After primary infection, human cytomegalovirus (HCMV) persists as a life-long latent infection, with host immunosuppression often resulting in clinical reactivation. During lytic infection, major changes in the expression of secreted cellular proteins (the secretome) occur that have profound effects on host-cell inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mason GM,Poole E,Sissons JG,Wills MR,Sinclair JH

更新日期：2012-09-04 00:00:00

abstract：:To study the permanency of response to selection for a quantitative character in finite populations and the nature of the genetic effects that contribute to this response, we have used the covariance between ancestors and descendents within populations. Effects and variances are defined for an initial equilibrium rand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cockerham CC,Tachida H

更新日期：1988-03-01 00:00:00

abstract：:Functional transcription elongation complexes can be formed by adding RNA polymerase in trans to a preformed nucleic acid construct. This construct consists of a double-stranded DNA fragment that contains a noncomplementary (permanent DNA bubble) region into which an RNA primer oligonucleotide has been hybridized. By ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daube SS,Hart CR,von Hippel PH

更新日期：1994-09-27 00:00:00

abstract：:Enhancing immune responses with immune-modulatory monoclonal antibodies directed to inhibitory immune receptors is a promising modality in cancer therapy. Clinical efficacy has been demonstrated with antibodies blocking inhibitory immune checkpoints such as cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) or PD-1/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Romano E,Kusio-Kobialka M,Foukas PG,Baumgaertner P,Meyer C,Ballabeni P,Michielin O,Weide B,Romero P,Speiser DE

更新日期：2015-05-12 00:00:00

abstract：:Platelet-derived growth factor (PDGF) isoforms lead to mitogenic, survival, and chemotactic responses in a variety of mesenchymal cell types during development and in the adult. We have studied the importance of phosphatidylinositol-3' kinase (PI3K) signaling in these responses by mutating the PI3K-binding sites in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heuchel R,Berg A,Tallquist M,Ahlén K,Reed RK,Rubin K,Claesson-Welsh L,Heldin CH,Soriano P

更新日期：1999-09-28 00:00:00

abstract：:The crossed strand-exchange form (Holliday structure, half chiasma) has been predicted as an intermediate in the genetic recombination of eukaryotes. We report here the detection of this form in the yeast plasmid, 2-micron DNA, isolated during meiosis. Physical mapping has previously suggested that two forms of 2-micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bell L,Byers B

更新日期：1979-07-01 00:00:00

abstract：:Photoadaptation, the ability to attenuate a light response on prolonged light exposure while remaining sensitive to escalating changes in light intensity, is essential for organisms to decipher time information appropriately, yet the underlying molecular mechanisms are poorly understood. In Neurospora crassa, VIVID (V...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen CH,DeMay BS,Gladfelter AS,Dunlap JC,Loros JJ

更新日期：2010-09-21 00:00:00

abstract：:Deletion of 13 amino acids from the carboxyl terminus of the 149-amino acid staphylococcal nuclease molecule results in a denatured, partly unfolded molecule that lacks persistent secondary structure but is compact under physiological conditions. Since the modification is a carboxyl-terminal deletion, it is argued tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flanagan JM,Kataoka M,Shortle D,Engelman DM

更新日期：1992-01-15 00:00:00

abstract：:Netrin-1 is critical for axonal pathfinding which shares similarities with formation of vascular network. Here we report that netrin-1 induction of angiogenesis is mediated by an increase in endothelial nitric oxide (NO*) production, which occurs via a DCC-dependent, ERK1/2-eNOS feed-forward mechanism. Exposure of mat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen A,Cai H

更新日期：2006-04-25 00:00:00

abstract：:Whether mitotic structures like the centrosome can self-organize from the regulated mobility of their dynamic protein components remains unclear. Here, we combine fluorescence spectroscopy and chemical genetics to study in living cells the diffusion of polo-like kinase 1 (PLK1), an enzyme critical for centrosome matur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahen R,Jeyasekharan AD,Barry NP,Venkitaraman AR

更新日期：2011-05-31 00:00:00

abstract：:A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)-expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A beta-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li XH,Carrington JC

更新日期：1995-01-17 00:00:00

abstract：:Never before have individuals had to adapt to social environments defined by such magnitudes of ethnic diversity and cultural differentiation. However, neurobiological evidence informing about strategies to reduce xenophobic sentiment and foster altruistic cooperation with outsiders is scarce. In a series of experimen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marsh N,Scheele D,Feinstein JS,Gerhardt H,Strang S,Maier W,Hurlemann R

更新日期：2017-08-29 00:00:00

abstract：:Compartmentalization of proteins into subcellular organelles in eukaryotic cells is a fundamental mechanism of regulating complex cellular functions. Many proteins of Plasmodium falciparum merozoites involved in invasion are compartmentalized into apical organelles. We have identified a new merozoite organelle that co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh S,Plassmeyer M,Gaur D,Miller LH

更新日期：2007-12-11 00:00:00

abstract：:Smallpox has played an unparalleled role in human history and remains a significant potential threat to public health. Despite the historical significance of this disease, we know little about the underlying pathophysiology or the virulence mechanisms of the causative agent, variola virus. To improve our understanding...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rubins KH,Hensley LE,Jahrling PB,Whitney AR,Geisbert TW,Huggins JW,Owen A,Leduc JW,Brown PO,Relman DA

更新日期：2004-10-19 00:00:00

abstract：:How do firing patterns in a cortical circuit change when inhibitory neurons are excited? We virally expressed an excitatory designer receptor exclusively activated by a designer drug (Gq-DREADD) in all inhibitory interneuron types of the CA1 region of the hippocampus in the rat. While clozapine N-oxide (CNO) activatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rogers S,Rozman PA,Valero M,Doyle WK,Buzsáki G

更新日期：2021-01-12 00:00:00

abstract：:The effect of pH on the membrane-fusion activity of Sendai virus was examined (pH 5.0-9.5) by using, as assays of activity, hemolysis of chicken erythrocytes and the fusion of baby hamster kidney (BHK-21) cells. Exposure of virus to basic pH increased fusion activity; the optimum pH was found to be approximately equal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu MC,Scheid A,Choppin PW

更新日期：1982-10-01 00:00:00

abstract：:The effects of messages initiated by one receptor on the covalent modification of a second receptor were studied by use of a technique for rapidly separating the receptors. Methylation of the bacterial-chemotactic serine receptor increases as a result of aspartate binding to the aspartate receptor. The aspartate-induc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanders DA,Koshland DE Jr

更新日期：1988-11-01 00:00:00

abstract：:Cell recognition molecules play a crucial role in the regulation of immune cells. We recently found that mast cells (MCs) express leukocyte recognition molecules, including ICAM-1 antigen, a natural ligand of LFA-1. We here report that interleukin 4 (IL-4), a pleiotropic cytokine and mast cell differentiation factor, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valent P,Bevec D,Maurer D,Besemer J,Di Padova F,Butterfield JH,Speiser W,Majdic O,Lechner K,Bettelheim P

更新日期：1991-04-15 00:00:00

abstract：:We present the use of our recently described multiple-loci polymorphic DNA markers ("alumorphs") for linkage mapping of the human genome. By using the polymerase chain reaction (PCR) with an Alu-specific primer we could reveal, in a single experiment, up to 20 genomic polymorphisms seen as the presence or absence of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zietkiewicz E,Labuda M,Sinnett D,Glorieux FH,Labuda D

更新日期：1992-09-15 00:00:00

abstract：:The cycles of carbon and oxygen at the Earth surface are intimately linked, where the burial of organic carbon into sediments represents a source of oxygen to the surface environment. This coupling is typically quantified through the isotope records of organic and inorganic carbon. Yet, the late Neoproterozoic Eon, th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bjerrum CJ,Canfield DE

更新日期：2011-04-05 00:00:00

abstract：:Neurons respond to sensory stimuli by altering the rate and temporal pattern of action potentials. These spike trains both encode and propagate information that guides behavior. Local inhibitory networks can affect the information encoded and propagated by neurons by altering correlations between different spike train...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giridhar S,Doiron B,Urban NN

更新日期：2011-04-05 00:00:00