Abstract:
:The effects of messages initiated by one receptor on the covalent modification of a second receptor were studied by use of a technique for rapidly separating the receptors. Methylation of the bacterial-chemotactic serine receptor increases as a result of aspartate binding to the aspartate receptor. The aspartate-induced methylation on the serine receptor is absent in a strain that lacks cheA and cheW genes and is not the result of physical interaction, such as the formation of heterodimers between the aspartate and serine receptors, or of alterations in the affinity of the serine receptor for the methyltransferase and the methylesterase. Serine-induced methylation of the serine receptor did not require cheA and cheW. A model is presented in which the receptor methylation level depends on the combination of (i) a ligand-induced conformational change on the receptor substrate of the methylation enzymes and (ii) an indirect cytoplasmic signal that operates through the methylesterase.
journal_name
Proc Natl Acad Sci U S Aauthors
Sanders DA,Koshland DE Jrdoi
10.1073/pnas.85.22.8425subject
Has Abstractpub_date
1988-11-01 00:00:00pages
8425-9issue
22eissn
0027-8424issn
1091-6490journal_volume
85pub_type
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