Enhancement of membrane-fusing activity of sendai virus by exposure of the virus to basic pH is correlated with a conformational change in the fusion protein.

abstract：:A promising strategy for DNA sequencing exploits transposons to provide mobile sites for the binding of sequencing primers. For such a strategy to be maximally efficient, the location and orientation of the transposon must be readily determined and the insertion sites should be randomly distributed. We demonstrate an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strausbaugh LD,Bourke MT,Sommer MT,Coon ME,Berg CM

更新日期：1990-08-01 00:00:00

abstract：:Administration of gamma-glutamylcysteinylglycyl monomethyl (or monoethyl) ester to mice leads to substantial increases in the levels of glutathione in the liver and kidney. Mice depleted of glutathione by treatment with buthionine sulfoximine, a potent inhibitor of gamma-glutamylcysteine synthetase, exhibited about a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Puri RN,Meister A

更新日期：1983-09-01 00:00:00

abstract：:We report substantially improved performance of high-power quantum cascade lasers (QCLs) by using epitaxial-side-down mounting that provides superior heat dissipation properties. We used aluminum nitride as the heatsink material and gold-tin eutectic solder. We have obtained continuous wave power output of 450 mW at 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsekoun A,Go R,Pushkarsky M,Razeghi M,Patel CK

更新日期：2006-03-28 00:00:00

abstract：:Differential rates of AIDS development and/or T4 lymphocyte depletion in HIV-1-infected individuals remain unexplained. The hypothesis that qualitative differences in selection pressure in vivo may account for different rates of disease progression was addressed in nine eligible study participants from a cohort of 315...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang WK,Essex M,McLane MF,Mayer KH,Hsieh CC,Brumblay HG,Seage G,Lee TH

更新日期：1996-06-25 00:00:00

abstract：:Insects living in the temperate zone enter a physiological state of arrested or slowed development to overcome an adverse season, such as winter. Developmental arrest, called diapause, occurs at a species-specific developmental stage, and embryonic and pupal diapauses have been extensively studied in mostly holometabo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miki T,Shinohara T,Chafino S,Noji S,Tomioka K

更新日期：2020-03-10 00:00:00

abstract：:Many groups of insects are specialists in exploiting sensory cues to locate food resources or conspecifics. To achieve orientation, bees and ants analyze the polarization pattern of the sky, male moths orient along the females' odor plume, and cicadas, grasshoppers, and crickets use acoustic signals to locate singing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poulet JF,Hedwig B

更新日期：2005-10-25 00:00:00

abstract：:Jasmonates (JAs) are plant-specific signaling molecules that steer a diverse set of physiological and developmental processes. Pathogen attack and wounding inflicted by herbivores induce the biosynthesis of these hormones, triggering defense responses both locally and systemically. We report on alterations in the tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauwels L,Morreel K,De Witte E,Lammertyn F,Van Montagu M,Boerjan W,Inzé D,Goossens A

更新日期：2008-01-29 00:00:00

abstract：:A convergent, highly stereoselective total synthesis of (-)-spinosyn A (1) is described. Key features of the synthesis include the transannular Diels-Alder reaction of macrocyclic pentaene 11 and the transannular Morita-Baylis-Hillman cyclization of 12 that generates tetracycle 26. The total synthesis of (-)-spinosyn ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mergott DJ,Frank SA,Roush WR

更新日期：2004-08-17 00:00:00

abstract：:Increased intestinal permeability has been observed in numerous human autoimmune diseases, including type-1 diabetes (T1D) and its' animal model, the BB-wor diabetic prone rat. We have recently described zonulin, a protein that regulates intercellular tight junctions. The objective of this study was to establish wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watts T,Berti I,Sapone A,Gerarduzzi T,Not T,Zielke R,Fasano A

更新日期：2005-02-22 00:00:00

abstract：:Ferredoxin was isolated from individual Leucaena glauca trees, and the distribution of amino acids at three of the four sites of sequence heterogeneity was determined. The results indicated that the most probable causes of the observed microheterogeneity are the presence of either nonallelic nuclear genes or differing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benson AM,Yasunobu KT

更新日期：1969-08-01 00:00:00

abstract：:Development of in vitro resistance to HN2 (also called mustargen or mechlorethamine hydrochloride), N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU), and cisplatin [cis-diamminedichloroplatinum(II)] was achieved in two human cell lines, the Raji/Burkitt lymphoma and a squamous cell carcinoma of the tongue. A 10- to 20-fol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frei E 3rd,Cucchi CA,Rosowsky A,Tantravahi R,Bernal S,Ervin TJ,Ruprecht RM,Haseltine WA

更新日期：1985-04-01 00:00:00

abstract：:All life on Earth is built of organic molecules, so the primordial sources of reduced carbon remain a major open question in studies of the origin of life. A variant of the alkaline-hydrothermal-vent theory for life's emergence suggests that organics could have been produced by the reduction of CO2 via H2 oxidation, f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hudson R,de Graaf R,Strandoo Rodin M,Ohno A,Lane N,McGlynn SE,Yamada YMA,Nakamura R,Barge LM,Braun D,Sojo V

更新日期：2020-09-15 00:00:00

abstract：:Cellular metabolism alters patterns of gene expression through a variety of mechanisms, including alterations in histone modifications and transcription factor activity. Nicotinamide adenine dinucleotide (NAD)-dependent proteins such as poly(ADP ribose) polymerases (PARPs) and sirtuin deacetylases play important roles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang J,Zhang B,Heath H,Galjart N,Wang X,Milbrandt J

更新日期：2010-12-14 00:00:00

abstract：:Lipid rafts are important signaling platforms in T cells. Little is known about their properties in human CD8(+) T cells. We studied polarization of lipid rafts by digital immunofluorescence microscopy in primary human T cells, using beads coated with anti-CD3 and anti-CD28 mAbs (CD3/28 beads). Unlike CD4(+) T cells, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovacs B,Maus MV,Riley JL,Derimanov GS,Koretzky GA,June CH,Finkel TH

更新日期：2002-11-12 00:00:00

abstract：:We find that histidine residues in proteins are major targets for reaction with the lipid peroxidation product 4-hydroxynon-2-enal (HNE). Reaction of insulin (which contains no sulfhydryl groups) with HNE leads to the generation of HNE-protein adducts, which are converted to radioactive derivatives upon subsequent tre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uchida K,Stadtman ER

更新日期：1992-05-15 00:00:00

abstract：:Improper function of voltage-gated sodium channels (NaVs), obligatory membrane proteins for bioelectrical signaling, has been linked to a number of human pathologies. Small-molecule agents that target NaVs hold considerable promise for treatment of chronic disease. Absent a comprehensive understanding of channel struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Tran R,Du Bois J

更新日期：2016-05-24 00:00:00

abstract：:In everyday listening, sound reaches our ears directly from a source as well as indirectly via reflections known as reverberation. Reverberation profoundly distorts the sound from a source, yet humans can both identify sound sources and distinguish environments from the resulting sound, via mechanisms that remain uncl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Traer J,McDermott JH

更新日期：2016-11-29 00:00:00

abstract：:Ribonucleoside diphosphate reductase from Escherichia coli and mammalian cells provides the deoxyribonucleoside triphosphates for DNA synthesis. The active enzyme contains a tyrosyl free radical whose formation requires oxygen. Earlier genetic evidence suggested that the enzyme is not required for anaerobic growth of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fontecave M,Eliasson R,Reichard P

更新日期：1989-04-01 00:00:00

abstract：:Venom peptide toxins such as conotoxins play a critical role in the characterization of nicotinic acetylcholine receptor (nAChR) structure and function and have potential as nervous system therapeutics as well. However, the lack of solved structures of conotoxins bound to nAChRs and the large size of these peptides ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leffler AE,Kuryatov A,Zebroski HA,Powell SR,Filipenko P,Hussein AK,Gorson J,Heizmann A,Lyskov S,Tsien RW,Poget SF,Nicke A,Lindstrom J,Rudy B,Bonneau R,Holford M

更新日期：2017-09-19 00:00:00

abstract：:The atomic buckling in 2D "Xenes" (such as silicene) fosters a plethora of exotic electronic properties such as a quantum spin Hall effect and could be engineered by external strain. Quantifying the buckling magnitude with subangstrom precision is, however, challenging, since epitaxially grown 2D layers exhibit comple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pawlak R,Drechsel C,D'Astolfo P,Kisiel M,Meyer E,Cerda JI

更新日期：2020-01-07 00:00:00

abstract：:The cDNA sequences encoding the adult alpha- and beta-globin polypeptides of a marsupial, the native cat Dasyurus viverrinus, have been cloned and their nucleotide sequences have been determined. Using these cDNA clones we have determined the chromosomal location of the native cat alpha- and beta-globin genes by in si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wainwright B,Hope R

更新日期：1985-12-01 00:00:00

abstract：:The importance of proper ion channel trafficking is underpinned by a number of channel-linked genetic diseases whose defect is associated with failure to reach the cell surface. Conceptually, it is reasonable to suggest that the function of ion channels depends critically on the precise subcellular localization and th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu L,Timofeyev V,Li N,Rafizadeh S,Singapuri A,Harris TR,Chiamvimonvat N

更新日期：2009-10-27 00:00:00

abstract：:1-Aminocyclopropane-1-carboxylate (ACC) oxidase catalyzes the oxidation of ACC to ethylene. Following conventional column fractionation, the enzyme was purified 180-fold to near homogeneity with a specific activity of 20 nmol/(mg.min). This purified enzyme preparation migrated as a single protein band with an apparent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong JG,Fernández-Maculet JC,Yang SF

更新日期：1992-10-15 00:00:00

abstract：:The formation of amyloid fibrils is a common biochemical characteristic that occurs in Alzheimer's disease and several other amyloidoses. The unifying structural feature of amyloid fibrils is their specific type of beta-sheet conformation that differentiates these fibrils from the products of normal protein folding re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Habicht G,Haupt C,Friedrich RP,Hortschansky P,Sachse C,Meinhardt J,Wieligmann K,Gellermann GP,Brodhun M,Götz J,Halbhuber KJ,Röcken C,Horn U,Fändrich M

更新日期：2007-12-04 00:00:00

abstract：:Chemical modifications of RNAs have long been established as key modulators of nonprotein-coding RNA structure and function in cells. There is a growing appreciation that messenger RNA (mRNA) sequences responsible for directing protein synthesis can also be posttranscriptionally modified. The enzymatic incorporation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyler DE,Franco MK,Batool Z,Wu MZ,Dubuke ML,Dobosz-Bartoszek M,Jones JD,Polikanov YS,Roy B,Koutmou KS

更新日期：2019-11-12 00:00:00

abstract：:High-valent iron-oxo species are thought to be intermediates in the catalytic cycles of oxygenases and peroxidases. An attractive route to these iron-oxo intermediates involves laser flash-quench oxidation of ferric hemes, as demonstrated by our work on the ferryl (compound II) and ferryl porphyrin radical cation (com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ener ME,Lee YT,Winkler JR,Gray HB,Cheruzel L

更新日期：2010-11-02 00:00:00

abstract：:Research on tuberculosis and leprosy was revolutionized by the development of a plasmid transformation system in the fast-growing surrogate, Mycobacterium smegmatis. This transformation system was made possible by the successful isolation of a M. smegmatis mutant strain mc(2)155, whose efficient plasmid transformation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Panas MW,Jain P,Yang H,Mitra S,Biswas D,Wattam AR,Letvin NL,Jacobs WR Jr

更新日期：2014-09-16 00:00:00

abstract：:One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun Q,Dong J,Yang W,Jin Y,Yang M,Wang Y,Wang PL,Hu Y,Tsien JZ

更新日期：2008-09-02 00:00:00

abstract：:The pathogenesis of tuberculous meningitis, a devastating complication of tuberculosis in man, is poorly understood. We previously reported that rabbits with experimental tuberculous meningitis were protected from death by a combination of antibiotics and thalidomide therapy. Survival was associated with inhibition of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsenova L,Bergtold A,Freedman VH,Young RA,Kaplan G

更新日期：1999-05-11 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease in the tropics and subtropics with high morbidity and mortality. The facultative intracellular bacterium induces host cell fusion through its type VI secretion system 5 (T6SS5) as an important part of its pathogenesis in mammalian h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ku JWK,Chen Y,Lim BJW,Gasser S,Crasta KC,Gan YH

更新日期：2020-07-07 00:00:00