Synthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry.

Abstract:

:Protein-based hydrogels have emerged as promising alternatives to synthetic hydrogels for biomedical applications, owing to the precise control of structure and function enabled by protein engineering. Nevertheless, strategies for assembling 3D molecular networks that carry the biological information encoded in full-length proteins remain underdeveloped. Here we present a robust protein gelation strategy based on a pair of genetically encoded reactive partners, SpyTag and SpyCatcher, that spontaneously form covalent isopeptide linkages under physiological conditions. The resulting "network of Spies" may be designed to include cell-adhesion ligands, matrix metalloproteinase-1 cleavage sites, and full-length globular proteins [mCherry and leukemia inhibitory factor (LIF)]. The LIF network was used to encapsulate mouse embryonic stem cells; the encapsulated cells remained pluripotent in the absence of added LIF. These results illustrate a versatile strategy for the creation of information-rich biomaterials.

authors

Sun F,Zhang WB,Mahdavi A,Arnold FH,Tirrell DA

doi

10.1073/pnas.1401291111

subject

Has Abstract

pub_date

2014-08-05 00:00:00

pages

11269-74

issue

31

eissn

0027-8424

issn

1091-6490

pii

1401291111

journal_volume

111

pub_type

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