Abstract:
:Protein-based hydrogels have emerged as promising alternatives to synthetic hydrogels for biomedical applications, owing to the precise control of structure and function enabled by protein engineering. Nevertheless, strategies for assembling 3D molecular networks that carry the biological information encoded in full-length proteins remain underdeveloped. Here we present a robust protein gelation strategy based on a pair of genetically encoded reactive partners, SpyTag and SpyCatcher, that spontaneously form covalent isopeptide linkages under physiological conditions. The resulting "network of Spies" may be designed to include cell-adhesion ligands, matrix metalloproteinase-1 cleavage sites, and full-length globular proteins [mCherry and leukemia inhibitory factor (LIF)]. The LIF network was used to encapsulate mouse embryonic stem cells; the encapsulated cells remained pluripotent in the absence of added LIF. These results illustrate a versatile strategy for the creation of information-rich biomaterials.
journal_name
Proc Natl Acad Sci U S Aauthors
Sun F,Zhang WB,Mahdavi A,Arnold FH,Tirrell DAdoi
10.1073/pnas.1401291111subject
Has Abstractpub_date
2014-08-05 00:00:00pages
11269-74issue
31eissn
0027-8424issn
1091-6490pii
1401291111journal_volume
111pub_type
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