Selection and identification of ligand peptides targeting a model of castrate-resistant osteogenic prostate cancer and their receptors.

abstract：:Metastasis of solid tumors is a key determinant of cancer patient survival. Targeting micrometastases using nanoparticles could offer a way to stop metastatic tumor growth before it causes excessive patient morbidity. However, nanoparticle delivery to micrometastases is difficult to investigate because micrometastases...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kingston BR,Syed AM,Ngai J,Sindhwani S,Chan WCW

更新日期：2019-07-23 00:00:00

abstract：:Although gamma/delta T cells express numerous in vitro functions similar to alpha/beta T cells, little is known about their biological functioning in vivo. Furthermore, it is unclear whether alpha/beta T cells and gamma/delta T cells act independently or in a coordinated way. In the present study, gamma/delta T cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann SH,Blum C,Yamamoto S

更新日期：1993-10-15 00:00:00

abstract：:Mosquitoes transmit devastating human diseases because they need vertebrate blood for egg development. Metabolism in female mosquitoes is tightly coupled with blood meal-mediated reproduction, which requires an extremely high level of energy consumption. Functional analysis has shown that major genes encoding for enzy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Hou Y,Saha TT,Pei G,Raikhel AS,Zou Z

更新日期：2017-03-28 00:00:00

abstract：:The fact that animal introns are not spliced out in plants suggests that recognition of pre-mRNA splice sites differs between the two kingdoms. In plants, little is known about proteins required for splicing, as no plant in vitro splicing system is available. Several essential splicing factors from animals, such as SF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopato S,Mayeda A,Krainer AR,Barta A

更新日期：1996-04-02 00:00:00

abstract：:An inversion loop seen in heteroduplex mapping of the DNA of mature Mu phage induced from a lysogen is observed also in defective lambda phage carrying one end of Mu. 14% of the DNA of Mu, including this region, designated the G loop, is shown to be to the right of all known genes in the prophage map. The inhomogeneou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daniell E,Boram W,Abelson J

更新日期：1973-07-01 00:00:00

abstract：:Two additional peptides with inhibin-like activity have been isolated from human seminal plasma. One consists of 52 amino acids and the other, 92 amino acids. They are designated alpha-inhibin-52 and alpha-inhibin-92. Sequence analyses show that the NH2-terminal 31 amino acids of alpha-inhibin-52 are identical to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li CH,Hammonds RG Jr,Ramasharma K,Chung D

更新日期：1985-06-01 00:00:00

abstract：:The catalytic generation of chiral ester enolate equivalents from α,β-unsaturated aldehydes with chiral N-hetereocyclic carbene catalysts makes possible highly enantioselective hetero-Diels-Alder reactions. The reactions proceed under simple, mild conditions with both aliphatic and aromatic substituted enals as substr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaeobamrung J,Kozlowski MC,Bode JW

更新日期：2010-11-30 00:00:00

abstract：:The vast majority of biologists engaged in evolutionary studies interpret virtually every aspect of biodiversity in adaptive terms. This narrow view of evolution has become untenable in light of recent observations from genomic sequencing and population-genetic theory. Numerous aspects of genomic architecture, gene st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lynch M

更新日期：2007-05-15 00:00:00

abstract：:Quinacrine's ability to reduce levels of pathogenic prion protein (PrP(Sc)) in mouse cells infected with experimentally adapted prions led to several unsuccessful clinical studies in patients with prion diseases, a 10-y investment to understand its mechanism of action, and the production of related compounds with expe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bian J,Kang HE,Telling GC

更新日期：2014-04-22 00:00:00

abstract：:Evidence indicates that the modulatory effects of the adrenergic stress hormone epinephrine as well as several other neuromodulatory systems on memory storage are mediated by activation of beta-adrenergic mechanisms in the amygdala. In view of our recent findings indicating that the amygdala is involved in mediating t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quirarte GL,Roozendaal B,McGaugh JL

更新日期：1997-12-09 00:00:00

abstract：:The dorsolateral striatum (DLS) is essential for motor and procedure learning, but the role of DLS spiny projection neurons (SPNs) of direct and indirect pathways, as marked, respectively, by D1 and D2 receptor (D1R and D2R) expression, remains to be clarified. Long-term two-photon calcium imaging of the same neuronal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheng MJ,Lu D,Shen ZM,Poo MM

更新日期：2019-05-28 00:00:00

abstract：:Mutations in Pink1, a gene encoding a Ser/Thr kinase with a mitochondrial-targeting signal, are associated with Parkinson's disease (PD), the most common movement disorder characterized by selective loss of dopaminergic neurons. The mechanism by which loss of Pink1 leads to neurodegeneration is not understood. Here we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Y,Gehrke S,Imai Y,Huang Z,Ouyang Y,Wang JW,Yang L,Beal MF,Vogel H,Lu B

更新日期：2006-07-11 00:00:00

abstract：:Evolution of an oxygenic atmosphere required primordial life to accommodate the toxicity associated with reactive oxygen species. We have characterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus. The amino acid sequence of this approximately 22-kDa protein shares little seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenheft B,Mosolf J,Willits D,Yeager M,Dryden KA,Young M,Douglas T

更新日期：2005-07-26 00:00:00

abstract：:The rapidly expanding network of roads into the Amazon is permanently altering the world's largest tropical forest. Most proposed road projects lack rigorous impact assessments or even basic economic justification. This study analyzes the expected environmental, social and economic impacts of 75 road projects, totalin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vilela T,Malky Harb A,Bruner A,Laísa da Silva Arruda V,Ribeiro V,Auxiliadora Costa Alencar A,Julissa Escobedo Grandez A,Rojas A,Laina A,Botero R

更新日期：2020-03-31 00:00:00

abstract：:The only known product of the Snyder-Theilen strain of feline sarcoma virus (ST-FeSV) is a 85,000-dalton protein, designated ST P85, that contains feline leukemia virus gag gene encoded proteins (p15, p12, and a fragment of p30) and a sarcoma virus-specific polypeptide. Antibodies directed against the latter immunopre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbacid M,Beemon K,Devare SG

更新日期：1980-09-01 00:00:00

abstract：:The molecular mechanism of desensitization of soluble guanylyl cyclase (sGC), the NO receptor, has long remained unresolved. Posttranslational modification and redox state have been postulated to affect sGC sensitivity to NO but evidence has been lacking. We now show that sGC can be S-nitrosylated in primary aortic sm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sayed N,Baskaran P,Ma X,van den Akker F,Beuve A

更新日期：2007-07-24 00:00:00

abstract：:Two-dimensional gel electrophoresis of immunoprecipitated human HLA-DR antigens from cells expressing the HLA-DR4 haplotype shows distinct clustering of beta-chain patterns. Six unique electrophoretic variants were observed among 17 HLA-DR4 homozygous cell lines (HCL) analyzed. These patterns correlate precisely with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nepom BS,Nepom GT,Mickelson E,Antonelli P,Hansen JA

更新日期：1983-11-01 00:00:00

abstract：:The COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and has spread worldwide, with millions of cases and more than 1 million deaths to date. The gravity of the situation mandates accelerated efforts to identify safe and effective vaccines. Here, we generated measles virus (M...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hörner C,Schürmann C,Auste A,Ebenig A,Muraleedharan S,Dinnon KH 3rd,Scholz T,Herrmann M,Schnierle BS,Baric RS,Mühlebach MD

更新日期：2020-12-22 00:00:00

abstract：:We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. O...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skurray RA,Nagaishi H,Clark AJ

更新日期：1976-01-01 00:00:00

abstract：:The interaction of interleukin 8 (IL-8) with heparin was studied by using synthetic IL-8 analogs with C- and N-terminal truncations. Elimination of the N-terminal region preceding the first cysteine, which constitutes the IL-8 receptor binding site, did not affect the affinity to heparin-Sepharose. Affinity, however, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webb LM,Ehrengruber MU,Clark-Lewis I,Baggiolini M,Rot A

更新日期：1993-08-01 00:00:00

abstract：:Methane emissions due to accidents in the oil and natural gas sector are very challenging to monitor, and hence are seldom considered in emission inventories and reporting. One of the main reasons is the lack of measurements during such events. Here we report the detection of large methane emissions from a gas well bl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pandey S,Gautam R,Houweling S,Denier van der Gon H,Sadavarte P,Borsdorff T,Hasekamp O,Landgraf J,Tol P,van Kempen T,Hoogeveen R,van Hees R,Hamburg SP,Maasakkers JD,Aben I

更新日期：2019-12-16 00:00:00

abstract：:4"'-Diazomalonyldigitoxin and its isomer, 3"'-diazomalonyldigitoxin, have been synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall C,Ruoho A

更新日期：1980-08-01 00:00:00

abstract：:Ion channels in excitable cells function in macromolecular complexes in which auxiliary proteins modulate the biophysical properties of the pore-forming subunits. Hyperpolarization-activated, cyclic nucleotide-sensitive HCN4 channels are critical determinants of membrane excitability in cells throughout the body, incl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peters CH,Myers ME,Juchno J,Haimbaugh C,Bichraoui H,Du Y,Bankston JR,Walker LA,Proenza C

更新日期：2020-07-28 00:00:00

abstract：:Understanding how changes in antibiotic consumption affect the prevalence of antibiotic resistance in bacterial pathogens is important for public health. In a number of bacterial species, including Streptococcus pneumoniae, the prevalence of resistance has remained relatively stable despite prolonged selection pressur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehtinen S,Blanquart F,Croucher NJ,Turner P,Lipsitch M,Fraser C

更新日期：2017-01-31 00:00:00

abstract：:The functional groups found among the RNA bases and in the phosphoribose backbone represent a limited repertoire from which to construct a ribozyme active site. This work investigates the possibility that simple RNA phosphodiester and hydroxyl functional groups could catalyze amide bond synthesis. Reaction of amine gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chamberlin SI,Merino EJ,Weeks KM

更新日期：2002-11-12 00:00:00

abstract：:The Escherichia coli flagellar motor senses the intracellular concentration of the response regulator CheY-P and responds by varying the bias between its counterclockwise (CCW) and clockwise (CW) rotational states. The response is ultrasensitive with a large Hill coefficient (approximately 10). Recently, the detailed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tu Y

更新日期：2008-08-19 00:00:00

abstract：:Electron spectroscopic imaging, a new technique that permits the quantitative detection of the spatial distributions of atomic elements at high resolution, has been applied to the epiphyseal zone of hypertrophy in the mouse for the visualization of calcium, phosphorus, and sulfur. Longitudinally sectioned epiphyseal g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arsenault AL,Ottensmeyer FP

更新日期：1983-03-01 00:00:00

abstract：:Historically, rate constants were determined in vitro and it was unknown whether they were valid for in vivo biological processes. Here, we bridge this gap by measuring binding dynamics between a pair of proteins in living HeLa cells. Binding of a β-lactamase to its protein inhibitor was initiated by microinjection an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillip Y,Kiss V,Schreiber G

更新日期：2012-01-31 00:00:00

abstract：:We investigated the manner in which the sea urchin larva takes up calcium from its body cavity into the primary mesenchymal cells (PMCs) that are responsible for spicule formation. We used the membrane-impermeable fluorescent dye calcein and alexa-dextran, with or without a calcium channel inhibitor, and imaged the la...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vidavsky N,Addadi S,Schertel A,Ben-Ezra D,Shpigel M,Addadi L,Weiner S

更新日期：2016-11-08 00:00:00

abstract：:Human diploid fibroblasts underwent lysis when resuspended in isotonic sucrose. After preparation of a granular fraction by differential centrifugation, lysosomes were almost completely purified by free-flow electrophoresis. Electron micrographs of the final fraction of fibroblast lysosomes (about 25-fold purified) sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harms E,Kern H,Schneider JA

更新日期：1980-10-01 00:00:00