Abstract:
:4"'-Diazomalonyldigitoxin and its isomer, 3"'-diazomalonyldigitoxin, have been synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of enzyme with both photolabels showed ouabain-protectable labeling of the alpha as well as the beta subunit. These data suggest that, in the purified eel enzyme, the alpha and beta subunits are in intimate contact, at least in the region of the third digitoxose of the "sugar-specific" binding site.
journal_name
Proc Natl Acad Sci U S Aauthors
Hall C,Ruoho Adoi
10.1073/pnas.77.8.4529subject
Has Abstractpub_date
1980-08-01 00:00:00pages
4529-33issue
8eissn
0027-8424issn
1091-6490journal_volume
77pub_type
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