当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon.

Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon.

Abstract：

:A revelation of the genomic age has been the contributions of the mobile DNA segments called transposable elements to chromosome structure, function, and evolution in virtually all organisms. Substantial fractions of vertebrate genomes derive from transposable elements, being dominated by retroelements that move via RNA intermediates. Although many of these elements have been inactivated by mutation, several active retroelements remain. Vertebrate genomes also contain substantial quantities and a high diversity of cut-and-paste DNA transposons, but no active representative of this class has been identified in mammals. Here we show that a cut-and-paste element called piggyBat, which has recently invaded the genome of the little brown bat (Myotis lucifugus) and is a member of the piggyBac superfamily, is active in its native form in transposition assays in bat and human cultured cells, as well as in the yeast Saccharomyces cerevisiae. Our study suggests that some DNA transposons are still actively shaping some mammalian genomes and reveals an unprecedented opportunity to study the mechanism, regulation, and genomic impact of cut-and-paste transposition in a natural mammalian host.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Mitra R,Li X,Kapusta A,Mayhew D,Mitra RD,Feschotte C,Craig NL

doi

10.1073/pnas.1217548110

subject

Has Abstract

pub_date

2013-01-02 00:00:00

pages

234-9

issue

1

eissn

0027-8424

issn

1091-6490

pii

1217548110

journal_volume

110

pub_type

杂志文章

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