Human lysosomes can be purified from diploid skin fibroblasts by free-flow electrophoresis.

abstract：:Oncogene-induced senescence (OIS) is an initial barrier to tumor development. Reactive oxygen species (ROS) is critical for oncogenic Ras OIS, but the downstream effectors to mediate ROS signaling are still relatively elusive. Senescent cells develop a senescence-associated secretory phenotype (SASP). However, the mec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang P,Han L,Shen H,Wang P,Lv C,Zhao G,Niu J,Xue L,Wang QJ,Tong T,Chen J

更新日期：2014-05-27 00:00:00

abstract：:In organisms from insects to vertebrates, Toll-like receptors (TLRs) are primary pathogen detectors that activate downstream pathways, specifically those that direct expression of innate immune effector genes. TLRs also have roles in development in many species. The sea anemone Nematostella vectensis is a useful cnida...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brennan JJ,Messerschmidt JL,Williams LM,Matthews BJ,Reynoso M,Gilmore TD

更新日期：2017-11-21 00:00:00

abstract：:The second critical endpoint in the basalt-H(2)O system was directly determined by a high-pressure and high-temperature X-ray radiography technique. We found that the second critical endpoint occurs at around 3.4 GPa and 770 °C (corresponding to a depth of approximately 100 km in a subducting slab), which is much shal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mibe K,Kawamoto T,Matsukage KN,Fei Y,Ono S

更新日期：2011-05-17 00:00:00

abstract：:"Functional connectivity" techniques are commonplace tools for studying brain organization. A critical element of these analyses is to distinguish variance due to neurobiological signals from variance due to nonneurobiological signals. Multiecho fMRI techniques are a promising means for making such distinctions based ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Power JD,Plitt M,Gotts SJ,Kundu P,Voon V,Bandettini PA,Martin A

更新日期：2018-02-27 00:00:00

abstract：:The exact role of the endocannabinoid system (ECS) during spermatogenesis has not been clarified. We used purified germ cell fractions representative of all phases of spermatogenesis and primary cultures of spermatogonia. This approach allowed the precise quantification of the cannabinoid receptor ligands, anandamide ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grimaldi P,Orlando P,Di Siena S,Lolicato F,Petrosino S,Bisogno T,Geremia R,De Petrocellis L,Di Marzo V

更新日期：2009-07-07 00:00:00

abstract：:The theory of "top-down" ecological regulation predicts that herbivory suppresses plant abundance, biomass, and survival but increases diversity through the disproportionate consumption of dominant species, which inhibits competitive exclusion. To date, these outcomes have been clear in aquatic ecosystems but not on l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jia S,Wang X,Yuan Z,Lin F,Ye J,Hao Z,Luskin MS

更新日期：2018-06-12 00:00:00

abstract：:Inhibin, a gonadal hormone capable of preferential suppression of pituitary follicle-stimulating hormone (FSH) secretion, has recently been purified. The major form of this protein is an alpha beta heterodimer encoded by two separate genes. In contrast to the FSH-suppressing action of the alpha beta heterodimer, the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsueh AJ,Dahl KD,Vaughan J,Tucker E,Rivier J,Bardin CW,Vale W

更新日期：1987-07-01 00:00:00

abstract：:Tetrahymena ribozymes hold promise for repairing genetic disorders but are largely limited by their modest splicing efficiency and low production of final therapeutic proteins. Ribozyme evolution in intact living mammalian cells would greatly facilitate the discovery of new ribozyme variants with high in vivo activity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasegawa S,Jackson WC,Tsien RY,Rao J

更新日期：2003-12-09 00:00:00

abstract：:AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu W,Kaminski R,Yang F,Zhang Y,Cosentino L,Li F,Luo B,Alvarez-Carbonell D,Garcia-Mesa Y,Karn J,Mo X,Khalili K

更新日期：2014-08-05 00:00:00

abstract：:Electron spin echo electron-nuclear double resonance (ESE-ENDOR) experiments performed on a broad radical electron paramagnetic resonance (EPR) signal observed in photosystem II particles depleted of Ca2+ indicate that this signal arises from the redox-active tyrosine YZ. The tyrosine EPR signal width is increased rel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gilchrist ML Jr,Ball JA,Randall DW,Britt RD

更新日期：1995-10-10 00:00:00

abstract：:Distributions of sulfur isotopes in geological samples would provide a record of atmospheric composition if the mechanism producing the isotope effects could be described quantitatively. We determined the UV absorption spectra of 32SO2, 33SO2, and 34SO2 and use them to interpret the geological record. The calculated i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ueno Y,Johnson MS,Danielache SO,Eskebjerg C,Pandey A,Yoshida N

更新日期：2009-09-01 00:00:00

abstract：:ATP-dependent RNA ligases are agents of RNA repair that join 3'-OH and 5'-PO4 RNA ends. Naegleria gruberi RNA ligase (NgrRnl) exemplifies a family of RNA nick-sealing enzymes found in bacteria, viruses, and eukarya. Crystal structures of NgrRnl at three discrete steps along the reaction pathway-covalent ligase-(lysyl-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Unciuleac MC,Goldgur Y,Shuman S

更新日期：2015-11-10 00:00:00

abstract：:We have identified new genomic alterations in the breast cancer cell line HCC1954, using high-throughput transcriptome sequencing. With 120 Mb of cDNA sequences, we were able to identify genomic rearrangement events leading to fusions or truncations of genes including MRE11 and NSD1, genes already implicated in oncoge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Q,Caballero OL,Levy S,Stevenson BJ,Iseli C,de Souza SJ,Galante PA,Busam D,Leversha MA,Chadalavada K,Rogers YH,Venter JC,Simpson AJ,Strausberg RL

更新日期：2009-02-10 00:00:00

abstract：:We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suggs SV,Wallace RB,Hirose T,Kawashima EH,Itakura K

更新日期：1981-11-01 00:00:00

abstract：:We report the discovery in Lake Cuitzeo in central Mexico of a black, carbon-rich, lacustrine layer, containing nanodiamonds, microspherules, and other unusual materials that date to the early Younger Dryas and are interpreted to result from an extraterrestrial impact. These proxies were found in a 27-m-long core as p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Israde-Alcántara I,Bischoff JL,Domínguez-Vázquez G,Li HC,DeCarli PS,Bunch TE,Wittke JH,Weaver JC,Firestone RB,West A,Kennett JP,Mercer C,Xie S,Richman EK,Kinzie CR,Wolbach WS

更新日期：2012-03-27 00:00:00

abstract：:WE REEXAMINED TWO QUESTIONS CONCERNING LYT ANTIGENS OF CYTOTOXIC T CELLS OF THE MOUSE: is Lyt-1 antigen expressed on cytotoxic effector cells and can cytotoxicity be blocked by antibody to Lyt antigens in the absence of added complement? A 3-hr (51)Cr-release assay with splenic effector cells and leukemia or myeloma t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakayama E,Shiku H,Stockert E,Oettgen HF,Old LJ

更新日期：1979-04-01 00:00:00

abstract：:Regulatory mechanisms for tissue repair and regeneration within damaged tissue have been extensively studied. However, the systemic regulation of tissue repair remains poorly understood. To elucidate tissue nonautonomous control of repair process, it is essential to induce local damage, independent of genetic manipula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kashio S,Obata F,Zhang L,Katsuyama T,Chihara T,Miura M

更新日期：2016-02-16 00:00:00

abstract：:Ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid have been attached covalently to the 5' terminus of the deoxynucleotide sequence C-A-C-A-A-T-T-C-C-A-C-A-C-A-A-C (16-mer) via an ethylenediamine linker. In the presence of Fe2+ and dithiothreitol, these reagents bring about the hybridization-depend...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu BC,Orgel LE

更新日期：1985-02-01 00:00:00

abstract：:The functional unit molecular size of the intestinal brush border membrane-bound Na+/glucose cotransporter was determined by radiation inactivation. Purified brush border membrane vesicles preserved in cryoprotectant buffer were irradiated (-135 degrees C) with high-energy electrons from a 13-MeV (1 eV = 1.602 x 10(-1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stevens BR,Fernandez A,Hirayama B,Wright EM,Kempner ES

更新日期：1990-02-01 00:00:00

abstract：:Recent epidemiological studies indicate beneficial effects of moderate ethanol consumption in ischemic heart disease. Most studies, however, focus on the effect of long-term consumption of ethanol. In this study, we determined whether brief exposure to ethanol immediately before ischemia also produces cardioprotection...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen CH,Gray MO,Mochly-Rosen D

更新日期：1999-10-26 00:00:00

abstract：:A sensitive test for kinetic unfolding intermediates in ribonuclease A (EC 3.1.27.5) is performed under conditions where the enzyme unfolds slowly (10 degrees C, pH 8.0, 4.5 M guanidinium chloride). Exchange of peptide NH protons (2H-1H) is used to monitor structural opening of individual hydrogen bonds during unfoldi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiefhaber T,Baldwin RL

更新日期：1995-03-28 00:00:00

abstract：:Observations on the structure and function of the ear in amphisbaenians have been extended to two new species: to Blanus cinereus of the family Amphisbaenidae and Diplometopon zarudnyi of the family Trogonophidae. Blanus, considered one of the most primitive of this group of reptiles, shows a distinctive form of sound...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gans C,Wever EG

更新日期：1975-04-01 00:00:00

abstract：:An ammonia-oxidizing, carbon-fixing archaeon, Candidatus "Nitrosopumilus maritimus," recently was isolated from a salt-water aquarium, definitively confirming that chemoautotrophy exists among the marine archaea. However, in other incubation studies, pelagic archaea also were capable of using organic carbon. It has re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ingalls AE,Shah SR,Hansman RL,Aluwihare LI,Santos GM,Druffel ER,Pearson A

更新日期：2006-04-25 00:00:00

abstract：:A single dose of N-methyl-N-nitrosourea given to sexually immature female Buf/N rats produces a high incidence of mammary adenocarcinomas. A large percentage of these tumors contain the Ha-ras oncogene, activated by a G----A transition at the second nucleotide of codon 12. Copenhagen rats, on the other hand, are compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu SJ,Archer MC

更新日期：1992-02-01 00:00:00

abstract：:Suspended aqueous aerosol droplets (<50 µm) are microreactors for many important atmospheric reactions. In droplets and other aquatic environments, pH is arguably the key parameter dictating chemical and biological processes. The nature of the droplet air/water interface has the potential to significantly alter drople...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wei H,Vejerano EP,Leng W,Huang Q,Willner MR,Marr LC,Vikesland PJ

更新日期：2018-07-10 00:00:00

abstract：:The polymerase chain reaction was used to amplify DNA surrounding the codon 12 region of the c-Ki-ras gene from C3H/10T1/2 cells and from a number of 3-methylcholanthrene (MCA)-transformed derivatives of these cells. Sequence analysis demonstrated that tumorigenic MCAC116/39 cells, known by DNA-mediated transfection t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen AC,Herschman HR

更新日期：1989-03-01 00:00:00

abstract：:This study describes the photochemical deposition of Co-based oxygen evolution catalysts on a semiconductor photoanode for use in solar oxygen evolution. In the photodeposition process, electron-hole pairs are generated in a semiconductor upon illumination and the photogenerated holes are used to oxidize Co(2+) ions t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steinmiller EM,Choi KS

更新日期：2009-12-08 00:00:00

abstract：:Routine dietary consumption of foods that contain aflatoxins is the second leading cause of environmental carcinogenesis worldwide. Aflatoxin-driven mutagenesis is initiated through metabolic activation of aflatoxin B1 (AFB1) to its epoxide form that reacts with N7 guanine in DNA. The resulting AFB1-N7-dG adduct under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin YC,Owen N,Minko IG,Lange SS,Tomida J,Li L,Stone MP,Wood RD,McCullough AK,Lloyd RS

更新日期：2016-11-29 00:00:00

abstract：:Long QT syndrome (LQT) is an autosomal dominant disorder that can cause sudden death from cardiac arrhythmias. We recently discovered that mutations in HERG, a K+-channel gene, cause chromosome 7-linked LQT. Heterologous expression of HERG in Xenopus oocytes revealed that HERG current was similar to a well-characteriz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanguinetti MC,Curran ME,Spector PS,Keating MT

更新日期：1996-03-05 00:00:00

abstract：:People who are more avoidant of pathogens are more politically conservative, as are nations with greater parasite stress. In the current research, we test two prominent hypotheses that have been proposed as explanations for these relationships. The first, which is an intragroup account, holds that these relationships ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tybur JM,Inbar Y,Aarøe L,Barclay P,Barlow FK,de Barra M,Becker DV,Borovoi L,Choi I,Choi JA,Consedine NS,Conway A,Conway JR,Conway P,Adoric VC,Demirci DE,Fernández AM,Ferreira DC,Ishii K,Jakšić I,Ji T,van Leeuwen

更新日期：2016-11-01 00:00:00