Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

abstract：:Human herpesvirus 6 (HHV-6) like other herpesviruses, expresses sequentially immediate early (IE), early, and late genes during lytic infection. Evidence of ability to establish latent infection has not been available, but by analogy with other herpesviruses it could be expected that IE genes that regulate and transac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotola A,Ravaioli T,Gonelli A,Dewhurst S,Cassai E,Di Luca D

更新日期：1998-11-10 00:00:00

abstract：:The transcription factor retinoic acid receptor beta(2) (RARbeta(2)) is a potent inhibitor of breast cancer cells in vitro, and studies suggest that RARbeta expression is lost in primary breast cancer. Although RARbeta(2) is selectively down-regulated at the mRNA level in breast tumor cells, we show that expression of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sommer KM,Chen LI,Treuting PM,Smith LT,Swisshelm K

更新日期：1999-07-20 00:00:00

abstract：:The relationship between the compositions of the Earth and chondritic meteorites is at the center of many important debates. A basic assumption in most models for the Earth's composition is that the refractory elements are present in chondritic proportions relative to each other. This assumption is now challenged by r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang S,Jacobsen SB,Mukhopadhyay S

更新日期：2013-03-26 00:00:00

abstract：:Arc repressor forms a homodimer in which the subunits intertwine to create a single globular domain. To obtain Arc sequences that fold preferentially as heterodimers, variants with surface patches of excess positive or negative charge were designed. Several but not all oppositely charged sequence pairs showed preferen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nohaile MJ,Hendsch ZS,Tidor B,Sauer RT

更新日期：2001-03-13 00:00:00

abstract：:Delayed implantation (embryonic diapause) occurs when the embryo at the blastocyst stage achieves a state of suspended animation. During this period, blastocyst growth is very slow, with minimal or no cell division. Nearly 100 mammals in seven different orders undergo delayed implantation, but the underlying molecular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamatani T,Daikoku T,Wang H,Matsumoto H,Carter MG,Ko MS,Dey SK

更新日期：2004-07-13 00:00:00

abstract：:CD45, a protein tyrosine phosphatase that regulates Src family kinases, is important for regulating T cell and B cell receptor signaling; however, little is known about how CD45 regulates immunoreceptor tyrosine-based activation motif (ITAM)-dependent natural killer (NK) cell receptor signaling and the resulting effec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hesslein DG,Takaki R,Hermiston ML,Weiss A,Lanier LL

更新日期：2006-05-02 00:00:00

abstract：:Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanchez JA,Pierce KE,Rice JE,Wangh LJ

更新日期：2004-02-17 00:00:00

abstract：:Somatostatin (somatotropin release-inhibiting factor; SRIF) is a tetradecapeptide present in brain, pancreas, gastrointestinal tract, and thyroid that inhibits the secretion or action of several hormones in these tissues. We observed that the toad urinary bladder contains concentrations of endogenous somatostatin (8.0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Forrest JN Jr,Reichlin S,Goodman DB

更新日期：1980-08-01 00:00:00

abstract：:The myocardin family proteins (myocardin, MRTF-A, and MRTF-B) are serum response factor (SRF) cofactors and potent transcription activators. Gene-ablation studies have indicated important developmental functions for myocardin family proteins primarily in regulation of cardiac and smooth muscle development. Using Xenop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meadows SM,Warkman AS,Salanga MC,Small EM,Krieg PA

更新日期：2008-02-05 00:00:00

abstract：:Cytochrome C is a mitochondrial protein that induces apoptosis when released into the cytosol or when added to cell-free extracts. Here we show that cells that overexpress the Bcl-2-related protein Bcl-xL fail to accumulate cytosolic cytochrome C or undergo apoptosis in response to genotoxic stress. Coimmunoprecipitat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kharbanda S,Pandey P,Schofield L,Israels S,Roncinske R,Yoshida K,Bharti A,Yuan ZM,Saxena S,Weichselbaum R,Nalin C,Kufe D

更新日期：1997-06-24 00:00:00

abstract：:The WWOX gene is a recently cloned tumor suppressor gene that spans the FRA16D fragile region. Wwox protein contains two WW domains that are generally known to mediate protein-protein interaction. Here we show that Wwox physically interacts via its first WW domain with the p53 homolog, p73. The tyrosine kinase, Src, p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aqeilan RI,Pekarsky Y,Herrero JJ,Palamarchuk A,Letofsky J,Druck T,Trapasso F,Han SY,Melino G,Huebner K,Croce CM

更新日期：2004-03-30 00:00:00

abstract：:Social behavior is often shaped by the rich storehouse of biographical information that we hold for other people. In our daily life, we rapidly and flexibly retrieve a host of biographical details about individuals in our social network, which often guide our decisions as we navigate complex social interactions. Even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Collins JA,Koski J,Nugiel T,Metoki A,Olson IR

更新日期：2017-04-18 00:00:00

abstract：:Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemical synthesis of si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu JY,DeRuiter SL,Turner DL

更新日期：2002-04-30 00:00:00

abstract：:Despite being engineered to avoid renal clearance, many cationic polymer (polycation)-based siRNA nanoparticles that are used for systemic delivery are rapidly eliminated from the circulation. Here, we show that a component of the renal filtration barrier--the glomerular basement membrane (GBM)--can disassemble cation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuckerman JE,Choi CH,Han H,Davis ME

更新日期：2012-02-21 00:00:00

abstract：:Like other guanine nucleotide-exchange proteins (GEPs) that activate ADP-ribosylation factor (ARF) GTPases, brefeldin A-inhibited GEP2, BIG2, contains an approximately 200-aa Sec7 domain that is responsible for this catalytic activity and its inhibition by brefeldin A. The Sec7 domain is located near the center of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Adamik R,Pacheco-Rodriguez G,Moss J,Vaughan M

更新日期：2003-02-18 00:00:00

abstract：:Preterm birth (PTB) complications are the leading cause of long-term morbidity and mortality in children. By using whole blood samples, we integrated whole-genome sequencing (WGS), RNA sequencing (RNA-seq), and DNA methylation data for 270 PTB and 521 control families. We analyzed this combined dataset to identify gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knijnenburg TA,Vockley JG,Chambwe N,Gibbs DL,Humphries C,Huddleston KC,Klein E,Kothiyal P,Tasseff R,Dhankani V,Bodian DL,Wong WSW,Glusman G,Mauldin DE,Miller M,Slagel J,Elasady S,Roach JC,Kramer R,Leinonen K,Linth

更新日期：2019-03-19 00:00:00

abstract：:We have studied androgen binding characteristics of cells for androgen insensitivity from an obligate heterozygote, who is the mother of three male pseudohermaphrodites with dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) receptor deficiency. Specific dihydrotestosterone binding by skin fibroblasts from he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer WJ 3rd,Migeon BR,Migeon CJ

更新日期：1975-04-01 00:00:00

abstract：:The transport of moisture in the tropics is a critical process for the global energy budget and on geologic timescales, has markedly influenced continental landscapes, migratory pathways, and biological evolution. Here we present a continuous, first-of-its-kind 1.3-My record of continental hydroclimate and lake-level ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyons RP,Scholz CA,Cohen AS,King JW,Brown ET,Ivory SJ,Johnson TC,Deino AL,Reinthal PN,McGlue MM,Blome MW

更新日期：2015-12-22 00:00:00

abstract：:The neonatal Fc receptor for IgG (FcRn) is a distant member of the MHC class I protein family. It binds IgG and albumin in a pH-dependent manner and protects these from catabolism by diverting them from a degradative fate in lysosomes. In addition, FcRn-mediated IgG transport across epithelial barriers is responsible ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiao SW,Kobayashi K,Johansen FE,Sollid LM,Andersen JT,Milford E,Roopenian DC,Lencer WI,Blumberg RS

更新日期：2008-07-08 00:00:00

abstract：:The glutathione S-transferases (GST) (glutathione transferase; EC 2.5.1.18) are a family of enzymes responsible for the metabolism of a broad range of xenobiotics and carcinogens. A cDNA clone containing the entire amino acid coding sequence of a human GST-2 subunit has been isolated using a lambda gt11 expression lib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Board PG,Webb GC

更新日期：1987-04-01 00:00:00

abstract：:The recombinant plasmid p102 based on pBR322 carrying approximately equal to 50% of the replicator proximal early region of simian virus 40 (SV40) DNA, including the viral origin of replication, has been constructed. It lacks a major part of the large tumor (T) antigen 3'-coding region, the T-antigen termination codon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horst J,Weckler C,Jacob E,Aulehla-Scholz C,Deppert W

更新日期：1982-08-01 00:00:00

abstract：:We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotem R,Paz GF,Homonnai ZT,Kalina M,Naor Z

更新日期：1990-09-01 00:00:00

abstract：:The Arabidopsis thaliana SOS2 and SOS3 genes are required for intracellular Na(+) and K(+) homeostasis and plant tolerance to high Na(+) and low K(+) environments. SOS3 is an EF hand type calcium-binding protein having sequence similarities with animal neuronal calcium sensors and the yeast calcineurin B. SOS2 is a se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halfter U,Ishitani M,Zhu JK

更新日期：2000-03-28 00:00:00

abstract：:The existence of immunoregulatory genes conferring dominant resistance to autoimmunity is well documented. In an effort to better understand the nature and mechanisms of action of these genes, we utilized the murine model of autoimmune orchitis as a prototype. When the orchitis-resistant strain DBA/2J is crossed with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meeker ND,Hickey WF,Korngold R,Hansen WK,Sudweeks JD,Wardell BB,Griffith JS,Teuscher C

更新日期：1995-06-06 00:00:00

abstract：:The beta 2-adrenergic receptor (beta 2AR) can be constitutively activated by mutations in the third intracellular loop. Whereas the wild-type receptor exists predominantly in an inactive conformation (R) in the absence of agonist, the mutant receptor appears to spontaneously adopt an active conformation (R*). We now d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pei G,Samama P,Lohse M,Wang M,Codina J,Lefkowitz RJ

更新日期：1994-03-29 00:00:00

abstract：:The primary structure of the Escherichia coli hemolysin polypeptide (HlyA) is used to predict intramolecular structures involved in the secretion and cytolytic activity of the molecule. The C-terminal region of HlyA contains a repeated, 8-amino acid chain represented by the consensus sequence Leu-Xaa-Gly-Gly-Xaa-Gly-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felmlee T,Welch RA

更新日期：1988-07-01 00:00:00

abstract：:Store-operated calcium entry (SOCE), an important mechanism of Ca2+ signaling in a wide range of cell types, is mediated by stromal interaction molecule (STIM), which senses the depletion of endoplasmic reticulum Ca2+ stores and binds and activates Orai channels in the plasma membrane. This inside-out mechanism of Ca2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim KM,Wijerathne T,Hur JH,Kang UJ,Kim IH,Kweon YC,Lee AR,Jeong SJ,Lee SK,Lee YY,Sim BW,Lee JH,Baig C,Kim SU,Chang KT,Lee KP,Park CY

更新日期：2018-05-15 00:00:00

abstract：:Understanding how changes in antibiotic consumption affect the prevalence of antibiotic resistance in bacterial pathogens is important for public health. In a number of bacterial species, including Streptococcus pneumoniae, the prevalence of resistance has remained relatively stable despite prolonged selection pressur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehtinen S,Blanquart F,Croucher NJ,Turner P,Lipsitch M,Fraser C

更新日期：2017-01-31 00:00:00

abstract：:Multicomponent therapies, originating through deliberate mixing of drugs in a clinical setting, through happenstance, and through rational design, have a successful history in a number of areas of medicine, including cancer, infectious diseases, and CNS disorders. We have developed a high-throughput screening method f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borisy AA,Elliott PJ,Hurst NW,Lee MS,Lehar J,Price ER,Serbedzija G,Zimmermann GR,Foley MA,Stockwell BR,Keith CT

更新日期：2003-06-24 00:00:00

abstract：:The reversible binding of the haptens 2,4-dinitrophenyllysine (DNP-lysine) and 2,4,6-trinitrophenyllysine (TNP-lysine) to either the nitrophenyl-binding myeloma protein MOPC-315 or to pooled mouse anti-DNP or anti-TNP antibodies produces large and characteristic extrinsic Cotton effects (in the circular dichroic spect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glaser M,Singer SJ

更新日期：1971-10-01 00:00:00