Abstract:
:Arc repressor forms a homodimer in which the subunits intertwine to create a single globular domain. To obtain Arc sequences that fold preferentially as heterodimers, variants with surface patches of excess positive or negative charge were designed. Several but not all oppositely charged sequence pairs showed preferential heterodimer formation. In the most successful design pair, alpha helix B of one subunit contained glutamic acids at positions 43, 46, 47, 48, and 50, whereas the other subunit contained lysines or arginines at these positions. A continuum electrostatic model captures many features of the experimental results and suggests that the most successful designs include elements of both positive and negative design.
journal_name
Proc Natl Acad Sci U S Aauthors
Nohaile MJ,Hendsch ZS,Tidor B,Sauer RTdoi
10.1073/pnas.051624498keywords:
subject
Has Abstractpub_date
2001-03-13 00:00:00pages
3109-14issue
6eissn
0027-8424issn
1091-6490pii
051624498journal_volume
98pub_type
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