phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.

abstract：:Landscape-scale reconstructions of ancient environments within the cradle of humanity may reveal insights into the relationship between early hominins and the changing resources around them. Many studies of Olduvai Gorge during Pliocene-Pleistocene times have revealed the presence of precession-driven wet-dry cycles a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sistiaga A,Husain F,Uribelarrea D,Martín-Perea DM,Ferland T,Freeman KH,Diez-Martín F,Baquedano E,Mabulla A,Domínguez-Rodrigo M,Summons RE

更新日期：2020-10-06 00:00:00

abstract：:Cactus, a Drosophila homologue of I kappa B, binds to and inhibits Dorsal, a homologue of the p50 and p65 components of NF-kappa B. We describe experiments in yeast with various Dorsal and Cactus derivatives showing that Cactus blocks the DNA binding and nuclear localization functions of Dorsal. In contrast, Dorsal's ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehming N,McGuire S,Brickman JM,Ptashne M

更新日期：1995-10-24 00:00:00

abstract：:Steroidogenic acute regulatory protein (StAR) appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. cDNAs encoding StAR were isolated from a human adrenal cortex library. Human StAR, coexpressed in COS-1 cells with cytochrome P450scc and adrenodoxin, increased pregnenolone synt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugawara T,Holt JA,Driscoll D,Strauss JF 3rd,Lin D,Miller WL,Patterson D,Clancy KP,Hart IM,Clark BJ

更新日期：1995-05-23 00:00:00

abstract：:The tissue-specific expression of the chicken liver cell adhesion molecule (L-CAM) was studied by generating transgenic mice. The rat insulin II promoter was fused to a chicken L-CAM cDNA or to chicken genomic L-CAM sequences. Mice carrying the cDNA showed no expression of L-CAM. Mice carrying L-CAM genomic sequences ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Begemann M,Tan SS,Cunningham BA,Edelman GM

更新日期：1990-11-01 00:00:00

abstract：:Mutations in the gene encoding the endothelin receptor type B (EDNRB) produce congenital aganglionic megacolon and pigment abnormalities in mice and humans. Here we report a naturally occurring null mutation of the EDNRB gene in spotting lethal (sl) rats, which exhibit aganglionic megacolon associated with white coat ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gariepy CE,Cass DT,Yanagisawa M

更新日期：1996-01-23 00:00:00

abstract：:In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maita N,Okada K,Hatakeyama K,Hakoshima T

更新日期：2002-02-05 00:00:00

abstract：:The acquisition of cell-surface urokinase plasminogen activator activity is a hallmark of malignancy. We generated an engineered anthrax toxin that is activated by cell-surface urokinase in vivo and displays limited toxicity to normal tissue but broad and potent tumoricidal activity. Native anthrax toxin protective an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu S,Aaronson H,Mitola DJ,Leppla SH,Bugge TH

更新日期：2003-01-21 00:00:00

abstract：:Various concentrations of L-dopa (levodopa, L-3,4-dihydroxyphenylalanine) were incorporated in the diets of young male Swiss albino mice, and the highest concentrations to which these mice became adapted within 3 weeks were determined. One such concentration administered indefinitely eventually induced a youthful appe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cotzias GC,Miller ST,Nicholson AR Jr,Maston WH,Tang LC

更新日期：1974-06-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) recently has been shown to give rise to genetic interference in Caenorhabditis elegans and also is likely to be the basis for phenotypic cosuppression in plants in certain instances. While constructing a plasmid vector for transfection of trypanosome cells, we serendipitously discovered tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ngô H,Tschudi C,Gull K,Ullu E

更新日期：1998-12-08 00:00:00

abstract：:Protein folding poses unique challenges for large, disordered proteins due to the low resolution of structural data accessible in experiment and on the basis of short time scales and limited sampling attainable in computation. Such molecules are uniquely suited to accelerated-sampling molecular dynamics algorithms due...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tarakanova A,Yeo GC,Baldock C,Weiss AS,Buehler MJ

更新日期：2018-07-10 00:00:00

abstract：:To explore the lethal, ataxic phenotype of complex I deficiency in Ndufs4 knockout (KO) mice, we inactivated Ndufs4 selectively in neurons and glia (NesKO mice). NesKO mice manifested the same symptoms as KO mice including retarded growth, loss of motor ability, breathing abnormalities, and death by approximately 7 wk...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quintana A,Kruse SE,Kapur RP,Sanz E,Palmiter RD

更新日期：2010-06-15 00:00:00

abstract：:Cytochrome c maturation in Escherichia coli requires the ccm operon, which encodes eight membrane proteins (CcmABCDEFGH). CcmE is a periplasmic heme chaperone that binds heme covalently and transfers it onto apocytochrome c in the presence of CcmF, CcmG, and CcmH. In this work we addressed the functions of the ccmABCD...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz H,Fabianek RA,Pellicioli EC,Hennecke H,Thöny-Meyer L

更新日期：1999-05-25 00:00:00

abstract：:Protein kinase A (PKA) is activated during sympathetic stimulation of the heart and phosphorylates key proteins involved in cardiac Ca(2+) handling, including the L-type Ca(2+) channel (Ca(V)1.2) and phospholamban (PLN). This results in acceleration and amplification of the beat-to-beat changes in cytosolic Ca(2+) in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones BW,Brunet S,Gilbert ML,Nichols CB,Su T,Westenbroek RE,Scott JD,Catterall WA,McKnight GS

更新日期：2012-10-16 00:00:00

abstract：:Pancreatic cancer is one of the most deadly cancers affecting the Western world. Because the disease is highly metastatic and difficult to diagnosis until late stages, the 5-y survival rate is around 5%. The identification of molecular cancer drivers is critical for furthering our understanding of the disease and deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mann KM,Ward JM,Yew CC,Kovochich A,Dawson DW,Black MA,Brett BT,Sheetz TE,Dupuy AJ,Australian Pancreatic Cancer Genome Initiative.,Chang DK,Biankin AV,Waddell N,Kassahn KS,Grimmond SM,Rust AG,Adams DJ,Jenkins NA,Copela

更新日期：2012-04-17 00:00:00

abstract：:The five alpha genes of herpes simplex virus 1 are the first set of genes to be expressed after infection. Previous studies have shown that alpha genes resident in eukaryotic cells are induced by infection with herpes simplex virus 1 or 2 but not by other herpesviruses and indicate that the alpha trans-inducing factor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pellett PE,McKnight JL,Jenkins FJ,Roizman B

更新日期：1985-09-01 00:00:00

abstract：:Agrobacterium is a unique model system as well as a major biotechnological tool for genetic manipulation of plant cells. It is still unknown, however, whether host cellular factors exist that are limiting for infection, and whether their overexpression in plant cells can increase the efficiency of the infection. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzfira T,Vaidya M,Citovsky V

更新日期：2002-08-06 00:00:00

abstract：:Type IV pili of Neisseria gonorrhoeae, the Gram-negative etiologic agent of gonorrhea, facilitate colonization of the human host. Gonococcal PilT, a protein belonging to a large family of molecules sharing a highly conserved nucleotide binding domain motif, has been shown to be dispensable for organelle biogenesis but...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolfgang M,Park HS,Hayes SF,van Putten JP,Koomey M

更新日期：1998-12-08 00:00:00

abstract：:The immunoglobulin heavy-chain variable region (VH) locus in a phylogenetically primitive teleost (Elops saurus) has been characterized by a strategy that relied initially on cross-hybridization between genomic VH segments and a murine VH probe. Using a homologous (Elops) VH probe and DNA sequencing, this gene family ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amemiya CT,Litman GW

更新日期：1990-01-01 00:00:00

abstract：:Let X(0) be an unknown M by N matrix. In matrix recovery, one takes n < MN linear measurements y(1),…,y(n) of X(0), where y(i) = Tr(A(T)iX(0)) and each A(i) is an M by N matrix. A popular approach for matrix recovery is nuclear norm minimization (NNM): solving the convex optimization problem min ||X||*subject to y(i) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donoho DL,Gavish M,Montanari A

更新日期：2013-05-21 00:00:00

abstract：:To adapt to habitat temperature, vertebrates have developed sophisticated physiological and ecological mechanisms through evolution. Transient receptor potential melastatin 8 (TRPM8) serves as the primary sensor for cold. However, how cold activates TRPM8 and how this sensor is tuned for thermal adaptation remain larg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang S,Lu X,Wang Y,Xu L,Chen X,Yang F,Lai R

更新日期：2020-04-14 00:00:00

abstract：:Antibody raised against the purified human erythrocyte glucose transporter specifically precipitated four proteins from normal and Rous sarcoma virus-transformed chicken embryo cells: a major protein of Mr 41,000 and minor proteins of Mr 68,000, 73,000, and 82,000. The Mr 41,000 and 82,000 proteins were found only in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salter DW,Baldwin SA,Lienhard GE,Weber MJ

更新日期：1982-03-01 00:00:00

abstract：:CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). CD11c can bind complement iC3b and mediate phagocytosis in vitro, for which it is also referred to as complement receptor 4. However, the functions of this prominent marker protein in DCs, especially in vivo, rema...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Wu H,An J,Ballantyne CM,Cyster JG

更新日期：2018-06-26 00:00:00

abstract：:We have previously shown that nuclear transcripts of the multifunctional enzyme, carbamoyl-phosphate synthetase, aspartate transcarbamylase, dihydroorotase RNA can be released from nuclei of Syrian hamster cells as compact ribonucleoprotein (RNP) particles that sediment at the 200S region in a sucrose gradient. The 20...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spann P,Feinerman M,Sperling J,Sperling R

更新日期：1989-01-01 00:00:00

abstract：:Four mouse POU domain genomic DNA clones--Brain-1, Brain-2, Brain-4, and Scip--and Brain-2 cDNA, which are expressed in adult brain, were cloned and the coding and noncoding regions of the genes were sequenced. The amino acid sequences of the four POU domains are highly conserved; sequences in other regions of the pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hara Y,Rovescalli AC,Kim Y,Nirenberg M

更新日期：1992-04-15 00:00:00

abstract：:A temperature-sensitive (ts) mutant, GA 907/7, was isolated after mutagen treatment of avian myeloblastosis virus. When bone marrow cells or secondary yolk sac macrophages were infected with GA 907/7, the expression of transformation was greatly reduced at 41 degrees C. The results of temperature-shift experiments sug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moscovici MG,Moscovici C

更新日期：1983-03-01 00:00:00

abstract：:Murine-virus-infected erythroleukemia cells cultured in a medium with dimethylsulfoxide or N,N-dimethylformamide are induced to differentiate to erythroid cells. A number of highly polar compounds have a similar effect in inducing erythroid differentiation of the virus-infected cells, as assayed by the appearance of h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanaka M,Levy J,Terada M,Breslow R,Rifkind RA,Marks PA

更新日期：1975-03-01 00:00:00

abstract：:We have isolated and partially sequenced a M(r) 160,000 glycoprotein whose rate of cycling to and from the adipocyte cell surface is enhanced by insulin in a manner apparently identical to the effect of insulin on GLUT4 cycling. Based on the protein sequence, we have prepared an antipeptide antibody against this prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kandror KV,Pilch PF

更新日期：1994-08-16 00:00:00

abstract：:Prophenoloxidases (PPOs) are key enzymes of the melanization reaction, which is a prominent defense mechanism of arthropods. The mosquito Aedes aegypti has ten PPO genes in the genome, four of which (PPO1, PPO3, PPO5, and PPO8) were expressed in response to microbial infection. Cactus depletion resulted in transcripti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zou Z,Shin SW,Alvarez KS,Bian G,Kokoza V,Raikhel AS

更新日期：2008-11-25 00:00:00

abstract：:Rhodopsin folding and assembly were investigated by expression of five bovine opsin gene fragments separated at points corresponding to proteolytic cleavage sites in the second or third cytoplasmic regions. The CH(1-146) and CH(147-348) gene fragments encode amino acids 1-146 and 147-348 of opsin, while the TH(1-240) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ridge KD,Lee SS,Yao LL

更新日期：1995-04-11 00:00:00

abstract：:IL-17-producing CD4(+) T helper (Th17) cells have recently been defined as a unique subset of proinflammatory helper cells whose development depends on signaling initiated by IL-6 and TGF-beta, autocrine activity of IL-21, activation of STAT3, and induction of the orphan nuclear receptor RORgammat. The maintenance, ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voo KS,Wang YH,Santori FR,Boggiano C,Wang YH,Arima K,Bover L,Hanabuchi S,Khalili J,Marinova E,Zheng B,Littman DR,Liu YJ

更新日期：2009-03-24 00:00:00