Expression of chicken liver cell adhesion molecule fusion genes in transgenic mice.

abstract：:Rat liver membrane adenylate cyclase (EC 4.6.1.1) that has been stimulated more than 10-fold by cholera toxin (choleragen) has a 3-fold greater sensitivity to stimulation by glucagon. Choleragen similarly increases the sensitivity of cyclase to other peptide (ACTH, vasoactive intestinal polypeptide) and nonpeptide (ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennett V,O'Keefe E,Cuatrecasaş P

更新日期：1975-01-01 00:00:00

abstract：:Genetic manipulation of the alpha(2A)-adrenergic receptor (alpha(2A)-AR) in mice has revealed the role of this subtype in numerous responses, including agonist-induced hypotension and sedation. Unexpectedly, alpha(2)-agonist treatment of mice heterozygous for the alpha(2A)-AR (alpha(2A)-AR(+/-)) lowers blood pressure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan CM,Wilson MH,MacMillan LB,Kobilka BK,Limbird LE

更新日期：2002-09-17 00:00:00

abstract：:Antibody modulation of T-cell coinhibitory (e.g., CTLA-4) or costimulatory (e.g., 4-1BB) receptors promotes clinical responses to a variety of cancers. Therapeutic cancer vaccination, in contrast, has produced limited clinical benefit and no curative therapies. The E6 and E7 oncoproteins of human papilloma virus (HPV)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartkowiak T,Singh S,Yang G,Galvan G,Haria D,Ai M,Allison JP,Sastry KJ,Curran MA

更新日期：2015-09-22 00:00:00

abstract：:The mannose receptor (MR) is an endocytic receptor involved in serum homeostasis and antigen presentation. Here, we identify the MR as a direct regulator of CD8(+) T-cell activity. We demonstrate that MR expression on dendritic cells (DCs) impaired T-cell cytotoxicity in vitro and in vivo. This regulatory effect of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schuette V,Embgenbroich M,Ulas T,Welz M,Schulte-Schrepping J,Draffehn AM,Quast T,Koch K,Nehring M,König J,Zweynert A,Harms FL,Steiner N,Limmer A,Förster I,Berberich-Siebelt F,Knolle PA,Wohlleber D,Kolanus W,Beyer M

更新日期：2016-09-20 00:00:00

abstract：:To explore protein space from a global perspective, we consider 9,710 SCOP (Structural Classification of Proteins) domains with up to 70% sequence identity and present all similarities among them as networks: In the "domain network," nodes represent domains, and edges connect domains that share "motifs," i.e., signifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nepomnyachiy S,Ben-Tal N,Kolodny R

更新日期：2014-08-12 00:00:00

abstract：:Little is known about the catalytic mechanism of the recently discovered ThyX family of flavin-dependent thymidylate synthases that are required for thymidylate (deoxythymidine 5'-monophosphate) synthesis in a large number of microbial species. Using a combination of site-directed mutagenesis and biochemical measureme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leduc D,Graziani S,Lipowski G,Marchand C,Le Maréchal P,Liebl U,Myllykallio H

更新日期：2004-05-11 00:00:00

abstract：:Agrobacterium tumefaciens causes crown gall tumors on various plants by delivering transferred DNA (T-DNA) and virulence proteins into host plant cells. Under laboratory conditions, the bacterium is widely used as a vector to genetically modify a wide range of organisms, including plants, yeasts, fungi, and algae. Var...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Q,Li X,Tu H,Pan SQ

更新日期：2017-03-14 00:00:00

abstract：:We have determined the complete primary structure of human hemopexin, a plasma beta-glycoprotein that specifically binds one heme with high affinity and transports it to hepatocytes for salvage of the iron. Human hemopexin (Mr approximately equal to 63,000) consists of a single polypeptide chain containing 439 amino a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takahashi N,Takahashi Y,Putnam FW

更新日期：1985-01-01 00:00:00

abstract：:Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Debey P,Balny C,Douzou P

更新日期：1973-09-01 00:00:00

abstract：:The circadian clock is a global regulatory mechanism that controls the expression of 50 to 80% of transcripts in mammals. Some of the genes controlled by the circadian clock are oncogenes or tumor suppressors. Among these Myc has been the focus of several studies which have investigated the effect of clock genes and p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Z,Selby CP,Yang Y,Lindsey-Boltz LA,Cao X,Eynullazada K,Sancar A

更新日期：2020-09-01 00:00:00

abstract：:Transcription factors (TFs) are primary regulators of gene expression in cells, where they bind specific genomic target sites to control transcription. Quantitative measurements of TF-DNA binding energies can improve the accuracy of predictions of TF occupancy and downstream gene expression in vivo and shed light on h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le DD,Shimko TC,Aditham AK,Keys AM,Longwell SA,Orenstein Y,Fordyce PM

更新日期：2018-04-17 00:00:00

abstract：:In vitro translation of Rous sarcoma virus (RSV) virion RNA in the messenger-dependent reticulocyte lysate system yielded polypeptides that were not synthesized by translation of RNA from a transformation-defective deletion mutant of RSV. These RSV-specific products migrated on sodium dodecyl sulfate/polyacrylamide ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beemon K,Hunter T

更新日期：1977-08-01 00:00:00

abstract：:Ouabain-sensitive uncoupled Na+ efflux has been studied in human, pig, and rat red cells and in vesicles containing reconstituted kidney Na+/K+ pumps obtained from these same species. The red cells from the different species gave qualitatively similar results; the uncoupled Na+ efflux was 15-30% of the Na+/K+ exchange...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martin WH,Richards DE,Marín R,Jack-Hays M,Hoffman JF

更新日期：1994-10-11 00:00:00

abstract：:The bacterial adaptive immune system CRISPR-Cas9 has been appropriated as a versatile tool for editing genomes, controlling gene expression, and visualizing genetic loci. To analyze Cas9's ability to bind DNA rapidly and specifically, we generated multiple libraries of potential binding partners for measuring the kine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyle EA,Andreasson JOL,Chircus LM,Sternberg SH,Wu MJ,Guegler CK,Doudna JA,Greenleaf WJ

更新日期：2017-05-23 00:00:00

abstract：:The interaction of interleukin 8 (IL-8) with heparin was studied by using synthetic IL-8 analogs with C- and N-terminal truncations. Elimination of the N-terminal region preceding the first cysteine, which constitutes the IL-8 receptor binding site, did not affect the affinity to heparin-Sepharose. Affinity, however, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webb LM,Ehrengruber MU,Clark-Lewis I,Baggiolini M,Rot A

更新日期：1993-08-01 00:00:00

abstract：:We have studied androgen binding characteristics of cells for androgen insensitivity from an obligate heterozygote, who is the mother of three male pseudohermaphrodites with dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) receptor deficiency. Specific dihydrotestosterone binding by skin fibroblasts from he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer WJ 3rd,Migeon BR,Migeon CJ

更新日期：1975-04-01 00:00:00

abstract：:Retinal dopaminergic amacrine neurons (DA neurons) play a central role in reconfiguring retinal function according to prevailing illumination conditions, yet the mechanisms by which light regulates their activity are poorly understood. We investigated the means by which sustained light responses are evoked in DA neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang DQ,Wong KY,Sollars PJ,Berson DM,Pickard GE,McMahon DG

更新日期：2008-09-16 00:00:00

abstract：:Assemblies formed by solid particles at interfaces have been widely studied because they serve as models of molecular phenomena, including molecular self-assembly. Solid particles adsorbed at interfaces also provide a means of stabilizing liquid-liquid emulsions and synthesizing materials with tunable mechanical, opti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koenig GM Jr,Lin IH,Abbott NL

更新日期：2010-03-02 00:00:00

abstract：:Previous studies have shown that the immunosuppressive and carcinogenic polycyclic aromatic hydrocarbon 7,12-dimethylbenz(a)anthracene (DMBA) impairs Ca(2+)-dependent transmembrane signaling in human and murine lymphocytes. The purpose of the present studies was to analyze potential mechanisms of immunosuppression by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Archuleta MM,Schieven GL,Ledbetter JA,Deanin GG,Burchiel SW

更新日期：1993-07-01 00:00:00

abstract：:How likely is it that someone would approve of using a nuclear weapon to kill millions of enemy civilians in the hope of ending a ground war that threatens thousands of American troops? Ask them how they feel about prosecuting immigrants, banning abortion, supporting the death penalty, and protecting gun rights and yo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slovic P,Mertz CK,Markowitz DM,Quist A,Västfjäll D

更新日期：2020-08-25 00:00:00

abstract：:Communities have an important role to play in biodiversity conservation. However, community-based conservation as a panacea, like government-based conservation as a panacea, ignores the necessity of managing commons at multiple levels, with vertical and horizontal interplay among institutions. The study of conservatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berkes F

更新日期：2007-09-25 00:00:00

abstract：:The intracellular localization of phytochrome was seen in dark-grown oat (Avena sativa L., cv. Garry) and rice (Oryza sativa L., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. Phytochrome is generally distributed throughout the cytoplasm in cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mackenzie JM Jr,Coleman RA,Briggs WR,Pratt LH

更新日期：1975-03-01 00:00:00

abstract：:We find that histidine residues in proteins are major targets for reaction with the lipid peroxidation product 4-hydroxynon-2-enal (HNE). Reaction of insulin (which contains no sulfhydryl groups) with HNE leads to the generation of HNE-protein adducts, which are converted to radioactive derivatives upon subsequent tre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uchida K,Stadtman ER

更新日期：1992-05-15 00:00:00

abstract：:Our understanding of the molecular control of many disease pathologies requires the identification of direct substrates targeted by specific protein kinases. Here we describe an integrated proteomic strategy, termed kinase assay linked with phosphoproteomics, which combines a sensitive kinase reaction with endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xue L,Wang WH,Iliuk A,Hu L,Galan JA,Yu S,Hans M,Geahlen RL,Tao WA

更新日期：2012-04-10 00:00:00

abstract：:Initiation of X chromosome inactivation requires the presence, in cis, of the X inactivation center (XIC). The Xist gene, which lies within the XIC region in both human and mouse and has the unique property of being expressed only from the inactive X chromosome in female somatic cells, is known to be essential for X i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heard E,Mongelard F,Arnaud D,Chureau C,Vourc'h C,Avner P

更新日期：1999-06-08 00:00:00

abstract：:The human epidermal growth factor receptor 2 (HER2) is specifically overexpressed in tumors of several cancers, including an aggressive form of breast cancer. It is therefore a target for both cancer diagnostics and therapy. The 58 amino acid residue Zher2 affibody molecule was previously engineered as a high-affinity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eigenbrot C,Ultsch M,Dubnovitsky A,Abrahmsén L,Härd T

更新日期：2010-08-24 00:00:00

abstract：:While cells within tissues generate and sense 3D states of strain, the current understanding of the mechanics of fibrous extracellular matrices (ECMs) stems mainly from uniaxial, biaxial, and shear tests. Here, we demonstrate that the multiaxial deformations of fiber networks in 3D cannot be inferred solely based on t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ban E,Wang H,Franklin JM,Liphardt JT,Janmey PA,Shenoy VB

更新日期：2019-04-02 00:00:00

abstract：:We report a combined thermodynamic and structural characterization of a DNA tetraplex. Using spectroscopic and calorimetric techniques, we demonstrate that d(TG3T) and d(TG3T2G3T), in the presence of K+, form stable tetramolecular complexes. From differential scanning calorimetry measurements, we obtain the following ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin R,Gaffney BL,Wang C,Jones RA,Breslauer KJ

更新日期：1992-09-15 00:00:00

abstract：:A mutation that confers resistance to the drug paromomycin is shown to be in the structural gene that codes for the ribosomal RNA in Tetrahymena. This observation was made by exploiting a variant of the ribosomal DNA that distorts amplification of this locus when a new somatic nucleus develops during conjugation. Beca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruns PJ,Katzen AL,Martin L,Blackburn EH

更新日期：1985-05-01 00:00:00

abstract：:A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmoduli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eid J,Sollner-Webb B

更新日期：1991-03-15 00:00:00