Enzyme assay in microsomes below zero degrees.

Abstract:

:Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .

authors

Debey P,Balny C,Douzou P

doi

10.1073/pnas.70.9.2633

subject

Has Abstract

pub_date

1973-09-01 00:00:00

pages

2633-6

issue

9

eissn

0027-8424

issn

1091-6490

journal_volume

70

pub_type

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