Abstract:
:The intracellular localization of phytochrome was seen in dark-grown oat (Avena sativa L., cv. Garry) and rice (Oryza sativa L., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. Phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. Within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absorbing form, becomes associated with discrete regions of the cell. These regions do not appear to be nuclei, plastids, or mitochondria. After phototransformation back to the red-absorbing form originally present, phytochrome slowly resumes its general distribution. It is possible that this discrete localization of the far-red-absorbing form of phytochrome represents a physiologically significant binding with a receptor site in the cell.
journal_name
Proc Natl Acad Sci U S Aauthors
Mackenzie JM Jr,Coleman RA,Briggs WR,Pratt LHdoi
10.1073/pnas.72.3.799keywords:
subject
Has Abstractpub_date
1975-03-01 00:00:00pages
799-803issue
3eissn
0027-8424issn
1091-6490journal_volume
72pub_type
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