A drug-resistant mutation in the ribosomal DNA of Tetrahymena.

Abstract:

:A mutation that confers resistance to the drug paromomycin is shown to be in the structural gene that codes for the ribosomal RNA in Tetrahymena. This observation was made by exploiting a variant of the ribosomal DNA that distorts amplification of this locus when a new somatic nucleus develops during conjugation. Because the allelic forms of this locus have a restriction endonuclease site polymorphism, it was possible to correlate drug resistance with presence of a specific allele. The genetic results have been confirmed by sequence analysis (presented elsewhere). Thus, the crosses presented here provide a unique opportunity to identify mutations in the ribosomal DNA.

authors

Bruns PJ,Katzen AL,Martin L,Blackburn EH

doi

10.1073/pnas.82.9.2844

subject

Has Abstract

pub_date

1985-05-01 00:00:00

pages

2844-6

issue

9

eissn

0027-8424

issn

1091-6490

journal_volume

82

pub_type

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