Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation.

abstract：:The Watson-Crick model for DNA duplex duplication proposes that the two parental chains separate and that each directs the synthesis of a complementary chain with which it is found associated after the duplication act. Previous experiments have left unchallenged alternative models which propose that in any single act ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russo VE,Stahl MM,Stahl FW

更新日期：1970-02-01 00:00:00

abstract：:Sirtuin 2 (SIRT2) is a sirtuin family deacetylase that directs acetylome signaling, protects genome integrity, and is a murine tumor suppressor. We show that SIRT2 directs replication stress responses by regulating the activity of cyclin-dependent kinase 9 (CDK9), a protein required for recovery from replication arres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang H,Park SH,Pantazides BG,Karpiuk O,Warren MD,Hardy CW,Duong DM,Park SJ,Kim HS,Vassilopoulos A,Seyfried NT,Johnsen SA,Gius D,Yu DS

更新日期：2013-08-13 00:00:00

abstract：:Cryoelectron microscopy and X-ray crystallography have recently been used to generate structural models that likely represent the unliganded closed-channel conformation and the fully liganded open-channel conformation of different members of the nicotinic-receptor superfamily. To characterize the structure of the clos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonzalez-Gutierrez G,Cuello LG,Nair SK,Grosman C

更新日期：2013-11-12 00:00:00

abstract：:Loss of the tumor suppressor, PTEN, is one of the most common findings in prostate cancer (PCa). This loss leads to overactive Akt signaling, which is correlated with increased metastasis and androgen independence. However, another tumor suppressor, inositol-polyphosphate 4-phosphatase type II (INPP4B), can partially ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaurasiya S,Wu W,Strom AM,Warner M,Gustafsson JÅ

更新日期：2020-10-20 00:00:00

abstract：:Voltage-gated Na(+) channels are composed of pore-forming alpha and auxiliary beta subunits. The majority of Na(+) channels in the heart contain tetrodotoxin (TTX)-insensitive Na(v)1.5 alpha subunits, but TTX-sensitive brain-type Na(+) channel alpha subunits are present and functionally important in the transverse tub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maier SK,Westenbroek RE,Yamanushi TT,Dobrzynski H,Boyett MR,Catterall WA,Scheuer T

更新日期：2003-03-18 00:00:00

abstract：:Male chickens with the henny feathering trait have a female feathering pattern. In two henny-feathered breeds, the Sebright bantam and the Golden Campine, the synthesis of estrogen is increased as a consequence of increased activity of aromatase, a cytochrome P-450 enzyme that converts androgen to estrogen. The activi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leshin M

更新日期：1985-05-01 00:00:00

abstract：:We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. O...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skurray RA,Nagaishi H,Clark AJ

更新日期：1976-01-01 00:00:00

abstract：:Alterations of human chromosome 8p occur frequently in many tumors. We identified a 1.5-Mb common region of allelic loss on 8p22 by allelotype analysis. cDNA selection allowed isolation of several genes, including FEZ1. The predicted Fez1 protein contained a leucine-zipper region with similarity to the DNA-binding dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishii H,Baffa R,Numata SI,Murakumo Y,Rattan S,Inoue H,Mori M,Fidanza V,Alder H,Croce CM

更新日期：1999-03-30 00:00:00

abstract：:The problem of demonstrating hemoglobin binding to the erythrocyte membrane in intact cells was approached by observing the quenching of fluorescent membrane probes by hemoglobin as a function of pH. This quenching was studied by measuring fluorescence intensities and decay rates of membrane-bound donors by both right...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisinger J,Flores J,Salhany JM

更新日期：1982-01-01 00:00:00

abstract：:Meiotic recombination in the yeast Saccharomyces cerevisiae is initiated by programmed double-strand breaks at selected sites throughout the genome (hotspots). alpha-Hotspots are binding sites for transcription factors. Double-strand breaks at alpha-hotspots require binding of transcription factor but not high levels ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abdullah MF,Borts RH

更新日期：2001-12-04 00:00:00

abstract：:Diffuse large B cell lymphomas (DLBCLs) often express BCL6, a transcriptional repressor required for the formation of normal germinal centers. In a subset of DLBCLs, BCL6 is deregulated by chromosomal translocations or aberrant somatic hypermutation; in other tumors, BCL6 expression may simply reflect germinal center ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polo JM,Juszczynski P,Monti S,Cerchietti L,Ye K,Greally JM,Shipp M,Melnick A

更新日期：2007-02-27 00:00:00

abstract：:Resting- or baseline-state low-frequency (0.01-0.2 Hz) brain activity is observed in fMRI, EEG, and local field potential recordings. These fluctuations were found to be correlated across brain regions and are thought to reflect neuronal activity fluctuations between functionally connected areas of the brain. However,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krishnan GP,González OC,Bazhenov M

更新日期：2018-06-26 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, self-recognition and the thereby promoted aggregation of thousands of cells into protective flocs is mediated by a family of cell-surface adhesins, the flocculins (Flo). Based on this social behavior FLO genes fulfill the definition of "greenbeard" genes, which direct coo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Veelders M,Brückner S,Ott D,Unverzagt C,Mösch HU,Essen LO

更新日期：2010-12-28 00:00:00

abstract：:Bacterial mutants have been isolated, called groN, that block phage development by interference with the action of the product of the phage N gene. lambdatrp phages, which depend on the N product for the synthesis of tryptophan enzymes, do not make these enzymes in groN bacteria. Two type of phage mutants have been is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgopoulos CP

更新日期：1971-12-01 00:00:00

abstract：:Intercropping, which grows at least two crop species on the same pieces of land at the same time, can increase grain yields greatly. Legume-grass intercrops are known to overyield because of legume nitrogen fixation. However, many agricultural soils are deficient in phosphorus. Here we show that a new mechanism of ove...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Li SM,Sun JH,Zhou LL,Bao XG,Zhang HG,Zhang FS

更新日期：2007-07-03 00:00:00

abstract：:Intermediate filaments-cytoplasmic filaments with a diameter of 8-9 nm-have been described in a large variety of cell types. In this study, the subunit protein of the neurofilament and the presumptive subunit of the astroglial filament are compared by immunological and biochemical methods and are found to be very simi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yen SH,Dahl D,Schachner M,Shelanski ML

更新日期：1976-02-01 00:00:00

abstract：:Rat carbonic anhydrase (CA) IV was purified by affinity chromatography and used to produce a specific antiserum in rabbits for immunolocalization studies in rat kidney. CA IV was localized in apical plasma membranes of the proximal convoluted tubule and the thick ascending limb of Henle. Both of these segments are inv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown D,Zhu XL,Sly WS

更新日期：1990-10-01 00:00:00

abstract：:Avian myelocytomatosis virus (MC29), a defective acute leukemia virus, has a broad oncogenic spectrum in vivo and transforms fibroblasts and hematopoietic target cells in vitro. We have used recombinant DNA technology to isolate and to characterize the sequences that are essential in the transformation process. Integr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lautenberger JA,Schulz RA,Garon CF,Tsichlis PN,Papas TS

更新日期：1981-03-01 00:00:00

abstract：:Host factor protein Cyclophilin A (CypA) regulates HIV-1 viral infectivity through direct interactions with the viral capsid, by an unknown mechanism. CypA can either promote or inhibit viral infection, depending on host cell type and HIV-1 capsid (CA) protein sequence. We have examined the role of conformational dyna...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu M,Hou G,Zhang H,Suiter CL,Ahn J,Byeon IJ,Perilla JR,Langmead CJ,Hung I,Gor'kov PL,Gan Z,Brey W,Aiken C,Zhang P,Schulten K,Gronenborn AM,Polenova T

更新日期：2015-11-24 00:00:00

abstract：:EWS (Ewing sarcoma) encodes an RNA/ssDNA binding protein that is frequently rearranged in a number of different cancers by chromosomal translocations. Physiologically, EWS has diverse and essential roles in various organ development and cellular processes. In this study, we uncovered a new role of EWS in mitochondrial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park JH,Kang HJ,Lee YK,Kang H,Kim J,Chung JH,Chang JS,McPherron AC,Lee SB

更新日期：2015-05-12 00:00:00

abstract：:The cytosolic concentration of free Ca2+ in bovine neutrophils was monitored by using the intracellular Ca2+ indicator quin2, 2-[[2-bis(acetylamino)-5-methylphenoxy]methyl-6-methoxy-8- bis(acetylamino)]quinoline. Neutrophils at rest have a cytosolic Ca2+ concentration of 85 +/- 5 nM, which in 2-4 min increases to 300-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gennaro R,Pozzan T,Romeo D

更新日期：1984-03-01 00:00:00

abstract：:A major part of the present understanding of the molecular basis of signal transduction has been gained from in vitro studies using classical biochemical methods. In this study, we used 31P NMR spectroscopy to investigate the response of live M2R mouse melanoma cells to stimulation by melanocyte-stimulating hormone (M...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Degani H,DeJordy JO,Salomon Y

更新日期：1991-02-15 00:00:00

abstract：:Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2 alpha, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have litt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrison RS,de Vellis J

更新日期：1981-11-01 00:00:00

abstract：:The supernatant fluid (conditioned medium) of cultured human vascular endothelial cells inhibits activation of Hageman factor (factor XII), whether by ellagic acid, bovine brain sulfatides, or bismuth subgallate; inhibition appears to be a property of one or more proteins in the culture supernates. This phenomenon may...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnoff OD,Everson B,Embury P,Ziats NP,Anderson JM,Emanuelson MM,Malemud CJ

更新日期：1991-12-01 00:00:00

abstract：:The B-cell antigen receptor is composed of membrane immunoglobulin sheathed by an alpha/beta heterodimer. The complex is noncovalently associated with protein kinase activity, and crosslinking of the receptor leads to capping and transmembrane signaling. Here we show that the sheath is not necessary either for this ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams GT,Peaker CJ,Patel KJ,Neuberger MS

更新日期：1994-01-18 00:00:00

abstract：:The DNA sequences of wheat Acc-1 and Acc-2 loci, encoding the plastid and cytosolic forms of the enzyme acetyl-CoA carboxylase, were analyzed with a view to understanding the evolution of these genes and the origin of the three genomes in modern hexaploid wheat. Acc-1 and Acc-2 loci from each of the wheats Triticum ur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chalupska D,Lee HY,Faris JD,Evrard A,Chalhoub B,Haselkorn R,Gornicki P

更新日期：2008-07-15 00:00:00

abstract：:PCSK9 encodes proprotein convertase subtilisin/kexin type 9a (PCSK9), a member of the proteinase K subfamily of subtilases. Missense mutations in PCSK9 cause an autosomal dominant form of hypercholesterolemia in humans, likely due to a gain-of-function mechanism because overexpression of either WT or mutant PCSK9 redu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rashid S,Curtis DE,Garuti R,Anderson NN,Bashmakov Y,Ho YK,Hammer RE,Moon YA,Horton JD

更新日期：2005-04-12 00:00:00

abstract：:Somatic mutations can now be quantified in almost any cell type in mice carrying bacterial genes in a lambda phage shuttle vector. Mutations induced in vivo are detectable ex vivo, after packaging host-cell DNA into phage that are grown on suitable bacteria. However, the transgenic DNA differs from many host loci in s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao KS,Urlando C,Heddle JA

更新日期：1993-11-15 00:00:00

abstract：:Two additional peptides with inhibin-like activity have been isolated from human seminal plasma. One consists of 52 amino acids and the other, 92 amino acids. They are designated alpha-inhibin-52 and alpha-inhibin-92. Sequence analyses show that the NH2-terminal 31 amino acids of alpha-inhibin-52 are identical to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li CH,Hammonds RG Jr,Ramasharma K,Chung D

更新日期：1985-06-01 00:00:00

abstract：:In this report, we explore the nature of the inductive stimuli leading to expression of the divergently regulated lymphokines interleukin 2 (IL-2) and interleukin 4 (IL-4). Elevation of cAMP levels blocks IL-2 induction while sparing IL-4 induction. These effects are gene-specific, not cell-specific, and can be observ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novak TJ,Rothenberg EV

更新日期：1990-12-01 00:00:00