Acc homoeoloci and the evolution of wheat genomes.

abstract：:A sensitive assay for detection of eukaryotic RNA polymerase II has been developed. This assay depends on the ability of polymerase II to elongate a small RNA primer, oligo(U), hybridized to a single-stranded homopolymeric DNA template, poly(dA). The poly(dA).oligo(U)-dependent RNA polymerase II from calf thymus has b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fradkin LG,Leong K,Morrow CD,Berk AJ,Dasgupta A

更新日期：1985-12-01 00:00:00

abstract：:Residential location is thought to influence people's well-being, but different individuals may value residential areas differently. We examined how life satisfaction and personality traits are geographically distributed within the UK London metropolitan area, and how the strength of associations between personality t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jokela M,Bleidorn W,Lamb ME,Gosling SD,Rentfrow PJ

更新日期：2015-01-20 00:00:00

abstract：:MicroRNAs (miRNAs) are 18- to 24-nt RNA molecules that regulate messenger RNAs (mRNAs). Posttranscriptional mechanisms regulate miRNA abundance during development as well as in cancer cells where miRNAs frequently exhibit dysregulated expression. The molecular mechanisms that govern the global efficiency of miRNA biog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang HW,Wentzel EA,Mendell JT

更新日期：2009-04-28 00:00:00

abstract：:Sympatric speciation has been controversial since it was first proposed as a mode of speciation. Subterranean blind mole rats (Spalacidae) are considered to speciate allopatrically or peripatrically. Here, we report a possible incipient sympatric adaptive ecological speciation in Spalax galili (2n = 52). The study mic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hadid Y,Tzur S,Pavlícek T,Šumbera R,Šklíba J,Lövy M,Fragman-Sapir O,Beiles A,Arieli R,Raz S,Nevo E

更新日期：2013-02-12 00:00:00

abstract：:Antigen.antibody complexes (Ag.Ab) prepared from (125)I-labeled bovine serum albumin and guinea pig anti-albumin were incubated at 37 degrees C for 30 min with normal human serum diluted optimally for binding (1:16) and then with autologous erythrocytes (RBC). After washing, RBC-bearing antigen.antibody.complement com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Medof ME,Prince GM,Mold C

更新日期：1982-08-01 00:00:00

abstract：:Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-neg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goosens VJ,Busch A,Georgiadou M,Castagnini M,Forest KT,Waksman G,Pelicic V

更新日期：2017-06-20 00:00:00

abstract：:The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stashenko P,Nadler LM,Hardy R,Schlossman SF

更新日期：1981-06-01 00:00:00

abstract：:Two independently arising alpha heavy chain mutants have been found to synthesize heavy chains with CH1 deletions of approximately equal extent. Both were isolated from heavy chain-producing variants of the mouse myeloma W3129 and demonstrate that it is possible to arrive at the heavy chain disease phenotype by the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dackowski W,Morrison SL

更新日期：1981-11-01 00:00:00

abstract：:A preparative two-dimensional polyacrylamide gel system was used to separate and purify the major Coomassie blue-stained proteins from the isolated rat liver nuclear matrix. Approximately 12 major proteins were consistently found. Of these, 5 proteins represented identified proteins, including nuclear lamins A, B, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakayasu H,Berezney R

更新日期：1991-11-15 00:00:00

abstract：:We are only just beginning to catalog the vast diversity of cell types in the cerebral cortex. Such categorization is a first step toward understanding how diversification relates to function. All cortical projection neurons arise from a uniform pool of progenitor cells that lines the ventricles of the forebrain. It i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heavner WE,Ji S,Notwell JH,Dyer ES,Tseng AM,Birgmeier J,Yoo B,Bejerano G,McConnell SK

更新日期：2020-10-06 00:00:00

abstract：:Mammalian SWI/SNF [also called BAF (Brg/Brahma-associated factors)] ATP-dependent chromatin remodeling complexes are essential for formation of the totipotent and pluripotent cells of the early embryo. In addition, subunits of this complex have been recovered in screens for genes required for nuclear reprogramming in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho L,Ronan JL,Wu J,Staahl BT,Chen L,Kuo A,Lessard J,Nesvizhskii AI,Ranish J,Crabtree GR

更新日期：2009-03-31 00:00:00

abstract：:Responses of tethered cells of Escherichia coli to impulse, step, exponential-ramp or exponentiated sine-wave stimuli are internally consistent, provided that allowance is made for the nonlinear effect of thresholds. This result confirms that wild-type cells exposed to stimuli in the physiological range make short-ter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segall JE,Block SM,Berg HC

更新日期：1986-12-01 00:00:00

abstract：:Variation in the multiplication rate of blood stage malaria parasites is often positively correlated with the severity of the disease they cause. The rodent malaria parasite Plasmodium yoelii yoelii has strains with marked differences in multiplication rate and pathogenicity in the blood. We have used genetic analysis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pattaradilokrat S,Culleton RL,Cheesman SJ,Carter R

更新日期：2009-04-28 00:00:00

abstract：:Recent evidence suggests that the Myc and Mad1 proteins are implicated in the regulation of the gene encoding the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. We have analyzed the in vivo interaction between endogenous c-Myc and Mad1 proteins and the hTERT promoter in HL60 cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu D,Popov N,Hou M,Wang Q,Björkholm M,Gruber A,Menkel AR,Henriksson M

更新日期：2001-03-27 00:00:00

abstract：:F1-ATPase, the catalytic domain of ATP synthase, synthesizes most of the ATP in living organisms. Running in reverse powered by ATP hydrolysis, this hexameric ring-shaped molecular motor formed by three αβ-dimers creates torque on its central γ-subunit. This reverse operation enables detailed explorations of the mecha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okazaki K,Hummer G

更新日期：2013-10-08 00:00:00

abstract：:Most of our knowledge about transmission at central synapses has been obtained by studying populations of synapses, but some important properties of synapses can be determined only by studying them individually. An important issue is whether a presynaptic action potential causes, at most, a single vesicle to be releas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conti R,Lisman J

更新日期：2003-04-15 00:00:00

abstract：:Scattering of light from dielectric particles whose size is on the order of an optical wavelength underlies a plethora of visual phenomena in nature and is a foundation for optical coatings and paints. Tailoring the internal nanoscale geometry of such "photonic particles" allows tuning their optical scattering charact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao G,Kaufman JJ,Shabahang S,Rezvani Naraghi R,Sukhov SV,Joannopoulos JD,Fink Y,Dogariu A,Abouraddy AF

更新日期：2016-06-21 00:00:00

abstract：:The spatial coordination of growth is of central importance for the regulation of plant tissue architecture. Individual layers, such as the epidermis, are clonally propagated and structurally maintained by symmetric cell divisions that are oriented along the plane of the layer. The developmental control of this proces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Enugutti B,Kirchhelle C,Oelschner M,Torres Ruiz RA,Schliebner I,Leister D,Schneitz K

更新日期：2012-09-11 00:00:00

abstract：:To facilitate genetic analysis of the human pathogenic parvovirus B19, we constructed a hybrid B19 viral genome in which the defective B19 inverted terminal repeats were replaced with the full-length inverted terminal repeats from a nonpathogenic human parvovirus, the adeno-associated virus 2 (AAV). The hybrid AAV-B19...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Srivastava CH,Samulski RJ,Lu L,Larsen SH,Srivastava A

更新日期：1989-10-01 00:00:00

abstract：:Aspartate transcarbamoylase (EC 2.1.3.2) from Escherichia coli contains six zinc ions per molecule. Renaturation studies of this allosteric enzyme and its isolated subunits show that the metal binding site is in the regulatory polypeptide chain. Ultraviolet difference spectra of the cadmium and zinc derivatives have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenbusch JP,Weber K

更新日期：1971-05-01 00:00:00

abstract：:Development of effective chemopreventive agents against prostate cancer (CaP) for humans requires conclusive evidence of their efficacy in animal models that closely emulates human disease. The autochthonous transgenic adenocarcinoma of the mouse prostate (TRAMP) model, which spontaneously develops metastatic CaP, is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gupta S,Hastak K,Ahmad N,Lewin JS,Mukhtar H

更新日期：2001-08-28 00:00:00

abstract：:The human rhodopsin gene is the locus for numerous alleles linked to the neurodegenerative disease retinitis pigmentosa. To facilitate the study of retinal degeneration and to test reagents designed to alter the structure and function of this gene, we have developed strains of mice whose native rhodopsin gene has been...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan F,Bradley A,Wensel TG,Wilson JH

更新日期：2004-06-15 00:00:00

abstract：:Seventeen monoclonal antibodies derived from fusions with spleen cells of mice immunized with established culture lines of renal cancers identified nine cell-surface antigenic systems. Six of the systems (gp160, S25, gp120r, gp120nr, gp115, and V1) represent antigens not previously described. The other three systems a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ueda R,Ogata S,Morrissey DM,Finstad CL,Szkudlarek J,Whitmore WF,Oettgen HF,Lloyd KO,Old LJ

更新日期：1981-08-01 00:00:00

abstract：:The bacterial actin homolog, MreB, forms helical cables within the cell that are required for maintenance of a rod shape. These helical structures are thought to be involved in the spatial organization of cell wall (peptidoglycan) synthesizing complexes of penicillin-binding proteins (PBPs). Here, we examined the role...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Divakaruni AV,Loo RR,Xie Y,Loo JA,Gober JW

更新日期：2005-12-20 00:00:00

abstract：:Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henderson TO,Costello AJ,Omachi A

更新日期：1974-06-01 00:00:00

abstract：:The tumor suppressor activity of p53 is regulated by interactions with the ubiquitin ligase HDM2 and the general transcriptional coactivators CBP and p300. Using NMR spectroscopy and isothermal titration calorimetry, we have dissected the binding interactions between the N-terminal transactivation domain (TAD) of p53,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferreon JC,Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-04-21 00:00:00

abstract：:The properties of glycine receptors (GlyRs) from newborn and adult rat spinal cord were studied in Xenopus oocytes injected with whole mRNA or the heavy (H) or light (L) mRNA fractions encoding their respective GlyRs. Mean open times and conductances of channels gated by H- or L-GlyRs were determined by noise analysis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morales A,Nguyen QT,Miledi R

更新日期：1994-04-12 00:00:00

abstract：:Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egorov TA,Svenson A,Rydén L,Carlsson J

更新日期：1975-08-01 00:00:00

abstract：:Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brabec RK,Peters BP,Bernstein IA,Gray RH,Goldstein IJ

更新日期：1980-01-01 00:00:00

abstract：:A total of 289,868 locus tests, based on 28 different protein phenotypes and using one-dimensional electrophoresis to detect variant proteins, has yielded one probable mutation in the offspring of "proximally exposed" parents, who received an estimated average gonadal exposure of 31 to 39 rem in the atomic bombings of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neel JV,Satoh C,Hamilton HB,Otake M,Goriki K,Kageoka T,Fujita M,Neriishi S,Asakawa J

更新日期：1980-07-01 00:00:00