Abstract:
:Mammalian SWI/SNF [also called BAF (Brg/Brahma-associated factors)] ATP-dependent chromatin remodeling complexes are essential for formation of the totipotent and pluripotent cells of the early embryo. In addition, subunits of this complex have been recovered in screens for genes required for nuclear reprogramming in Xenopus and mouse embryonic stem cell (ES) morphology. However, the mechanism underlying the roles of these complexes is unclear. Here, we show that BAF complexes are required for the self-renewal and pluripotency of mouse ES cells but not for the proliferation of fibroblasts or other cells. Proteomic studies reveal that ES cells express distinctive complexes (esBAF) defined by the presence of Brg (Brahma-related gene), BAF155, and BAF60A, and the absence of Brm (Brahma), BAF170, and BAF60C. We show that this specialized subunit composition is required for ES cell maintenance and pluripotency. Our proteomic analysis also reveals that esBAF complexes interact directly with key regulators of pluripotency, suggesting that esBAF complexes are specialized to interact with ES cell-specific regulators, providing a potential explanation for the requirement of BAF complexes in pluripotency.
journal_name
Proc Natl Acad Sci U S Aauthors
Ho L,Ronan JL,Wu J,Staahl BT,Chen L,Kuo A,Lessard J,Nesvizhskii AI,Ranish J,Crabtree GRdoi
10.1073/pnas.0812889106subject
Has Abstractpub_date
2009-03-31 00:00:00pages
5181-6issue
13eissn
0027-8424issn
1091-6490pii
0812889106journal_volume
106pub_type
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