On the transfer of information from old to new chains of DNA duplexes in phage lambda: destruction of heterozygotes.

abstract：:The expression and integration of membrane proteins into vesicle membranes is a critical step in the design of cell-mimetic biosensors, bioreactors, and artificial cells. While membrane proteins have been integrated into a variety of nonnatural membranes, the effects of the chemical and physical properties of these ve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacobs ML,Boyd MA,Kamat NP

更新日期：2019-03-05 00:00:00

abstract：:We review a program of research that uses neuroimaging techniques to determine the functional and neural architecture of human working memory. A first set of studies indicates that verbal working memory includes a storage component, which is implemented neurally by areas in the left-hemisphere posterior parietal corte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith EE,Jonides J

更新日期：1998-09-29 00:00:00

abstract：:Defects in clearing apoptotic debris disrupt tissue and immunological homeostasis, leading to autoimmune and inflammatory diseases. Herein, we report that macrophages from lupus-prone MRL/lpr mice have impaired lysosomal maturation, resulting in heightened ROS production and attenuated lysosomal acidification. Impaire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Monteith AJ,Kang S,Scott E,Hillman K,Rajfur Z,Jacobson K,Costello MJ,Vilen BJ

更新日期：2016-04-12 00:00:00

abstract：:Benthic foraminifers inhabit a wide range of aquatic environments including open marine, brackish, and freshwater environments. Here we show that several different and diverse foraminiferal groups (miliolids, rotaliids, textulariids) and Gromia, another taxon also belonging to Rhizaria, accumulate and respire nitrates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Piña-Ochoa E,Høgslund S,Geslin E,Cedhagen T,Revsbech NP,Nielsen LP,Schweizer M,Jorissen F,Rysgaard S,Risgaard-Petersen N

更新日期：2010-01-19 00:00:00

abstract：:Diffusion in the plasma membrane of living cells is often found to display anomalous dynamics. However, the mechanism underlying this diffusion pattern remains highly controversial. Here, we study the physical mechanism underlying Kv2.1 potassium channel anomalous dynamics using single-molecule tracking. Our analysis ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weigel AV,Simon B,Tamkun MM,Krapf D

更新日期：2011-04-19 00:00:00

abstract：:Division of labor is fundamental to the success of all societies. The most striking examples are the physically polymorphic worker castes in social insects with clear morphological adaptations to different roles. These polymorphic worker castes have previously been thought to be a classic example of nongentically cont...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes WO,Sumner S,Van Borm S,Boomsma JJ

更新日期：2003-08-05 00:00:00

abstract：:Cryopreservation is widely used to maintain backups of cells as it enables the semipermanent storage of cells. During the freezing process, ice crystals that are generated inside and outside the cells can lethally damage the cells. All conventional cryopreservation methods use at least one cryoprotective agent (CPA) t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama Y,Shinose M,Watanabe H,Yamada S,Kanda Y

更新日期：2019-04-16 00:00:00

abstract：:Perilipin coats the lipid droplets of adipocytes and is thought to have a role in regulating triacylglycerol hydrolysis. To study the role of perilipin in vivo, we have created a perilipin knockout mouse. Perilipin null (peri(-/-)) and wild-type (peri(+/+)) mice consume equal amounts of food, but the adipose tissue ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tansey JT,Sztalryd C,Gruia-Gray J,Roush DL,Zee JV,Gavrilova O,Reitman ML,Deng CX,Li C,Kimmel AR,Londos C

更新日期：2001-05-22 00:00:00

abstract：:The G protein-coupled m1 and m3 muscarinic acetylcholine receptors increase tyrosine phosphorylation of several proteins, including the focal adhesion-associated proteins paxillin and focal adhesion kinase (FAK), but the mechanism is not understood. Activation of integrins during adhesion of cells to extracellular mat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slack BE

更新日期：1998-06-23 00:00:00

abstract：:A high-resolution compositional map of glass-forming ability (GFA) in the Ni-Cr-Nb-P-B system is experimentally determined along various compositional planes. GFA is shown to be a piecewise continuous function formed by intersecting compositional subsurfaces, each associated with a nucleation pathway for a specific cr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Na JH,Demetriou MD,Floyd M,Hoff A,Garrett GR,Johnson WL

更新日期：2014-06-24 00:00:00

abstract：:Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biggin MD,Gibson TJ,Hong GF

更新日期：1983-07-01 00:00:00

abstract：:The structure of the gene encoding human hexabrachion (tenascin) has been determined from overlapping clones isolated from a human genomic bacteriophage library. The genomic inserts were characterized by restriction mapping, Southern blot analysis, PCR, and DNA sequencing. The coding region of the hexabrachion gene sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gulcher JR,Nies DE,Alexakos MJ,Ravikant NA,Sturgill ME,Marton LS,Stefansson K

更新日期：1991-11-01 00:00:00

abstract：:It has recently been recognized that human serum contains a protein that specifically binds human growth hormone (hGH). This protein has the same restricted specificity for hGH as the membrane-bound GH receptor. To determine whether the GH-binding protein is a derivative of, or otherwise related to, the GH receptor, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daughaday WH,Trivedi B

更新日期：1987-07-01 00:00:00

abstract：:To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauer S,Vasu P,Persson S,Mort AJ,Somerville CR

更新日期：2006-07-25 00:00:00

abstract：:Human genetic diversity in southern Europe is higher than in other regions of the continent. This difference has been attributed to postglacial expansions, the demic diffusion of agriculture from the Near East, and gene flow from Africa. Using SNP data from 2,099 individuals in 43 populations, we show that estimates o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Botigué LR,Henn BM,Gravel S,Maples BK,Gignoux CR,Corona E,Atzmon G,Burns E,Ostrer H,Flores C,Bertranpetit J,Comas D,Bustamante CD

更新日期：2013-07-16 00:00:00

abstract：:A technique is described for isolation of plasmid DNA in closed and open circular double-stranded forms from bacterial cells, by use of ATP-dependent deoxyribonuclease purified from Micrococcus luteus. This DNase, acting only upon linear DNA molecules, degrades all bacterial chromosomal DNA extracted in the linear for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mukai T,Matsubara K,Takagi Y

更新日期：1973-10-01 00:00:00

abstract：:A three-plasmid system for heterologous expression of 6-deoxyerythronolide B synthase (DEBS) has been developed to facilitate combinatorial biosynthesis of polyketides made by type I modular polyketide synthases (PKSs). The eryA PKS genes encoding the three DEBS subunits were individually cloned into three compatible ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xue Q,Ashley G,Hutchinson CR,Santi DV

更新日期：1999-10-12 00:00:00

abstract：:Loss or reduction in function of tumor suppressor genes contributes to tumorigenesis. Here, by allelic DNA copy number analysis using single-nucleotide polymorphism genotyping array and mass spectrometry, we report homozygous deletion in glioblastoma multiformes at chromosome 13q21, where DACH1 gene is located. We fou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe A,Ogiwara H,Ehata S,Mukasa A,Ishikawa S,Maeda D,Ueki K,Ino Y,Todo T,Yamada Y,Fukayama M,Saito N,Miyazono K,Aburatani H

更新日期：2011-07-26 00:00:00

abstract：:This paper provides transcendental and algebraic framework for the classification of identities expressing pi and other logarithms of algebraic numbers as rapidly convergent generalized hypergeometric series in rational parameters. Algebraic and arithmetic relations between values of p+1Fp hypergeometric functions and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chudnovsky DV,Chudnovsky GV

更新日期：1998-03-17 00:00:00

abstract：:Fumarase C was strongly induced by paraquat in a parental strain of Escherichia coli but was not induced in a strain lacking the soxRS response. Moreover, a strain that constitutively expresses the soxRS regulon contained more fumarase C than did the parental strain. The Mn-containing superoxide dismutase and glucose-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liochev SI,Fridovich I

更新日期：1992-07-01 00:00:00

abstract：:NGC 7027 shows the richest spectrum of any known gaseous nebula. In the region of the spectrum surveyed we have measured lines of ions of H, He, C, N, O, F, Ne, Mg, Si, P, S, Cl, Ar, K, Ca, Mn, and Fe using the new Hamilton Echelle Spectrograph of Lick Observatory with a charge-coupled device (CCD) detector. Since a C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aller LH,Keyes CD

更新日期：1988-04-01 00:00:00

abstract：:What are the origins of humans' capacity to represent social relations? We approached this question by studying human infants' understanding of social dominance as a stable relation. We presented infants with interactions between animated agents in conflict situations. Studies 1 and 2 targeted expectations of stabilit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mascaro O,Csibra G

更新日期：2012-05-01 00:00:00

abstract：:Experiments were designed to determine the interrelationship between cyclic AMP and Ca(2+) during the processes of sperm capacitation and the acrosome reaction. In minimal culture media containing pyruvate and lactate as substrates, guinea pig spermatozoa required a minimum of 1.0-1.5 hr to capacitate in the presence ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyne RV,Garbers DL

更新日期：1979-11-01 00:00:00

abstract：:The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we report a simple altern...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valéry C,Paternostre M,Robert B,Gulik-Krzywicki T,Narayanan T,Dedieu JC,Keller G,Torres ML,Cherif-Cheikh R,Calvo P,Artzner F

更新日期：2003-09-02 00:00:00

abstract：:A three-dimensional model of the protein arrangement in the Escherichia coli 30S ribosome was constructed by using computerized multidimensional scaling of immunoelectron microscope data. This enabled data comparison between the new electron microscope technique and other methods such as crosslinking, chemical protect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaffney PT,Craven G

更新日期：1978-07-01 00:00:00

abstract：:We describe a quantum mechanics/molecular mechanics investigation of the guanidinoacetate methyltransferase catalyzed reaction, which shows that proton transfer from guanidinoacetate (GAA) to Asp-134 and methyl transfer from S-adenosyl-L-methionine (AdoMet) to GAA are concerted. By self-consistent-charge density funct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang X,Bruice TC

更新日期：2006-10-31 00:00:00

abstract：:The enhanced disease resistance 1 (edr1) mutation of Arabidopsis confers resistance to powdery mildew disease caused by the fungus Erysiphe cichoracearum. Resistance mediated by the edr1 mutation is correlated with induction of several defense responses, including host cell death. Double mutant analysis revealed that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frye CA,Tang D,Innes RW

更新日期：2001-01-02 00:00:00

abstract：:The multidimensional free energy surface for a small fast folding helical protein is explored based on first-principle calculations. The model represents the 46-residue segment from fragment B of staphylococcal protein A. The relationship between collapse and tertiary structure formation, and the order of collapse and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo Z,Brooks CL 3rd,Boczko EM

更新日期：1997-09-16 00:00:00

abstract：:The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berlyn MB,Last RL,Fink GR

更新日期：1989-06-01 00:00:00

abstract：:The taxonomic status of Neanderthals lies at the center of the modern human origins debate. Proponents of the single-origin model often view this group as a distinct species with little or no contribution to the evolution of modern humans. Adherents to the regional continuity model consider Neanderthals a subspecies o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harvati K,Frost SR,McNulty KP

更新日期：2004-02-03 00:00:00