Abstract:
:Bacterial mutants have been isolated, called groN, that block phage development by interference with the action of the product of the phage N gene. lambdatrp phages, which depend on the N product for the synthesis of tryptophan enzymes, do not make these enzymes in groN bacteria. Two type of phage mutants have been isolated that can overcome the groN block. One type makes an altered N product, the other contains an N-bypass mutation. The groN mutation is closely linked to the rifamycin-resistance locus in Escherichia coli. Purified RNA polymerase from the groN mutant is less activated by salt and more sensitive to rifamycin than is the polymerase from gro(+). This suggests that the groN mutation produces a structural change in the bacterial RNA polymerase such that it can no longer interact properly with the phage N product.
journal_name
Proc Natl Acad Sci U S Aauthors
Georgopoulos CPdoi
10.1073/pnas.68.12.2977subject
Has Abstractpub_date
1971-12-01 00:00:00pages
2977-81issue
12eissn
0027-8424issn
1091-6490journal_volume
68pub_type
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