Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus.

abstract：:NaeI is a remarkable type II restriction endonuclease. It must bind two recognition sequences to cleave DNA, forms a covalent protein-DNA intermediate, and is only 1 aa change away from topoisomerase and recombinase activity. The latter activities apparently derive from reactivation of a cryptic DNA ligase active site...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colandene JD,Topal MD

更新日期：1998-03-31 00:00:00

abstract：:Solvent additives (cosolvents, osmolytes) modulate biochemical reactions if, during the course of the reaction, there is a change in preferential interactions of solvent components with the reacting system. Preferential interactions can be expressed in terms of preferential binding of the cosolvent or its preferential...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Timasheff SN

更新日期：2002-07-23 00:00:00

abstract：:XMAP215/Dis1 family proteins positively regulate microtubule growth. Repeats at their N termini, called TOG domains, are important for this function. While TOG domains directly bind tubulin dimers, it is unclear how this interaction translates to polymerase activity. Understanding the functional roles of TOG domains i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Widlund PO,Stear JH,Pozniakovsky A,Zanic M,Reber S,Brouhard GJ,Hyman AA,Howard J

更新日期：2011-02-15 00:00:00

abstract：:Since at least the 1920s, it has been reported that common chimpanzees (Pan troglodytes) differ from humans in being capable of exceptional feats of "super strength," both in the wild and in captive environments. A mix of anecdotal and more controlled studies provides some support for this view; however, a critical re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Neill MC,Umberger BR,Holowka NB,Larson SG,Reiser PJ

更新日期：2017-07-11 00:00:00

abstract：:Parasitic plants share a common anatomical feature, the haustorium. Haustoria enable both infection and nutrient transfer, which often leads to growth penalties for host plants and yield reduction in crop species. Haustoria also reciprocally transfer substances, such as RNA and proteins, from parasite to host, but the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spallek T,Melnyk CW,Wakatake T,Zhang J,Sakamoto Y,Kiba T,Yoshida S,Matsunaga S,Sakakibara H,Shirasu K

更新日期：2017-05-16 00:00:00

abstract：:The action of calmodulin (CaM) on target proteins is important for a variety of cellular functions. We demonstrate here, however, that the presence of a CaM-binding site on a protein does not necessarily imply a functional effect. The alpha-subunit of the cGMP-gated cation channel of human retinal cones has a CaM-bind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grunwald ME,Zhong H,Lai J,Yau KW

更新日期：1999-11-09 00:00:00

abstract：:Locus content maps are derived from monosomic or disomic chromosomes broken by radiation, shearing, or other clastogen, the fragments being distributed among clones by dilution or incorporation into the cells of another species and scored for segregation of markers. Locus content maps provide evidence about radiosensi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teague JW,Collins A,Morton NE

更新日期：1996-10-15 00:00:00

abstract：:The Alu element has been a major source of new exons during primate evolution. Thousands of human genes contain spliced exons derived from Alu elements. However, identifying Alu exons that have acquired genuine biological functions remains a major challenge. We investigated the creation and establishment of Alu exons ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen S,Lin L,Cai JJ,Jiang P,Kenkel EJ,Stroik MR,Sato S,Davidson BL,Xing Y

更新日期：2011-02-15 00:00:00

abstract：:The early steps in the biosynthesis of Taxol involve the cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene followed by cytochrome P450-mediated hydroxylation at C5, acetylation of this intermediate, and a second cytochrome P450-dependent hydroxylation at C10 to yield taxadien-5 alpha-acetoxy-10 beta-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoendorf A,Rithner CD,Williams RM,Croteau RB

更新日期：2001-02-13 00:00:00

abstract：:Flagellar polypeptides of Chlamydomonas reinhardtii were analyzed in two-dimensions by isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate. In addition to flagellar tubulin, over 130 polypeptides were resolved and 100 of these were identified as axonemal components in wild-type organisms...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Piperno G,Huang B,Luck DJ

更新日期：1977-04-01 00:00:00

abstract：:The study of development has relied primarily on the isolation of mutations in genes with specific functions in development and on the comparison of their expression patterns in normal and mutant phenotypes. Comparative evolutionary analyses can complement these approaches. Phylogenetic analyses of Sonic hedgehog (Shh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zardoya R,Abouheif E,Meyer A

更新日期：1996-11-12 00:00:00

abstract：:Several findings suggest that signals from tyrosine kinases are transduced, at least in part, through ras proteins. These findings include (i) blockage of the transforming activity of constitutively active tyrosine kinases by inhibiting ras function and (ii) genetic screens in Caenorhabditis elegans and in Drosophila ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowtell D,Fu P,Simon M,Senior P

更新日期：1992-07-15 00:00:00

abstract：:Investigation of retinal neurochemistry in a well-defined chick model of form-deprivation myopia indicated that dopamine and its metabolite 3,4-dihydroxyphenylacetic acid are reduced in myopic as compared to control eyes. The reduction in retinal dopamine is evident only during light adaptation and is accompanied by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone RA,Lin T,Laties AM,Iuvone PM

更新日期：1989-01-01 00:00:00

abstract：:The role of anti-peripheral nerve myelin antibody (anti-PNM Ab) in the pathogenesis of acquired demyelination of peripheral nerve is unclear, in part, due to the poor correlation between antibody and disease activity. Previous studies show that only 27-50% of patients with acute demyelinating neuropathy or Guillain-Ba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koski CL,Humphrey R,Shin ML

更新日期：1985-02-01 00:00:00

abstract：:Infants' sensitivity to contingent reactivity as an indicator of intentional agency has been demonstrated by numerous referential gaze-following studies. Here we propose that variability of signal sequences in a turn-taking exchange provides an informative cue for infants to recognize interactions that may involve com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tauzin T,Gergely G

更新日期：2019-07-30 00:00:00

abstract：:The flatworm Macrostomum lignano features a duo-gland adhesive system that allows it to repeatedly attach to and release from substrates in seawater within a minute. However, little is known about the molecules involved in this temporary adhesion. In this study, we show that the attachment of M. lignano relies on the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wunderer J,Lengerer B,Pjeta R,Bertemes P,Kremser L,Lindner H,Ederth T,Hess MW,Stock D,Salvenmoser W,Ladurner P

更新日期：2019-03-05 00:00:00

abstract：:Capsaicin is a unique alkaloid of the plant kingdom restricted to the genus Capsicum. Capsaicin is the pungency factor, a bioactive molecule of food and of medicinal importance. Capsaicin is useful as a counterirritant, antiarthritic, analgesic, antioxidant, and anticancer agent. Capsaicin biosynthesis involves conden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prasad BC,Kumar V,Gururaj HB,Parimalan R,Giridhar P,Ravishankar GA

更新日期：2006-09-05 00:00:00

abstract：:The sperm receptors of the ascidian oocyte are located at the outer surface of the vitelline coat (formerly called the chorion). The fucose residues are the receptor's most important components for sperm recognition and binding. We asked whether the fucosyl-containing glycoproteins of the vitelline coat are a product ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosati F,Cotelli F,De Santis R,Monroy A,Pinto MR

更新日期：1982-03-01 00:00:00

abstract：:Legionella pneumophila causes a potentially fatal form of pneumonia by replicating within macrophages in the Legionella-containing vacuole (LCV). Bacterial survival and proliferation within the LCV rely on hundreds of secreted effector proteins comprising high functional redundancy. The vacuolar membrane-localized Mav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Christenson ET,Isaac DT,Yoshida K,Lipo E,Kim JS,Ghirlando R,Isberg RR,Banerjee A

更新日期：2019-09-03 00:00:00

abstract：:Hippocampal mossy fibers, which are the axons of dentate granule cells, form powerful excitatory synapses onto the proximal dendrites of CA3 pyramidal cells. It has long been known that high-affinity binding sites for kainate, a glutamate receptor agonist, are present on mossy fibers. Here we summarize recent experime...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmitz D,Mellor J,Frerking M,Nicoll RA

更新日期：2001-09-25 00:00:00

abstract：:Eukaryote cell division features a chromosome compaction-decompaction cycle that is synchronized with their physical and topological segregation. It has been proposed that lengthwise compaction of chromatin into mitotic chromosomes via loop extrusion underlies the compaction-segregation/resolution process. We analyze ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brahmachari S,Marko JF

更新日期：2019-12-10 00:00:00

abstract：:Micron-scale robots require systems that can morph into arbitrary target configurations controlled by external agents such as heat, light, electricity, and chemical environment. Achieving this behavior using conventional approaches is challenging because the available materials at these scales are not programmable lik...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agrawal M,Glotzer SC

更新日期：2020-04-21 00:00:00

abstract：:The oscillator of the circadian clock of cyanobacteria is composed of three proteins, KaiA, KaiB, and KaiC, which together generate a self-sustained ∼24-h rhythm of phosphorylation of KaiC. The mechanism propelling this oscillator has remained elusive, however. We show that stacking interactions between the CI and CII...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang YG,Tseng R,Kuo NW,LiWang A

更新日期：2012-10-16 00:00:00

abstract：:Two-electron oxidation of cobaltous octaethylporphyrin [Co(II)(Et)(8)P] yields a stable pi-cation radical [Co(III)(Et)(8)P](2+.), the optical spectrum of which exhibits spectral changes dependent upon the nature of the counterion. Comparison of these spectra with those of Compounds I of horseradish peroxidase and cata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dolphin D,Forman A,Borg DC,Fajer J,Felton RH

更新日期：1971-03-01 00:00:00

abstract：:We have designed three zinc-finger proteins with different DNA binding specificities. The design strategy combines a consensus zinc-finger framework sequence with previously characterized recognition regions such that the specificity of each protein is predictable. The first protein consists of three identical zinc fi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Desjarlais JR,Berg JM

更新日期：1993-03-15 00:00:00

abstract：:The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pelham RJ,Rodgers L,Hall I,Lucito R,Nguyen KC,Navin N,Hicks J,Mu D,Powers S,Wigler M,Botstein D

更新日期：2006-12-26 00:00:00

abstract：:The posterodorsal part of the medial nucleus of the amygdala (MeAp) receives its major sensory input from the accessory olfactory bulb and projects massively to the medial preoptic nucleus and other sexually dimorphic hypothalamic nuclei thought to play key roles in mediating steroid-sensitive reproductive functions. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simerly RB,Young BJ,Capozza MA,Swanson LW

更新日期：1989-06-01 00:00:00

abstract：:There are numerous studies on the immune response against malignant human tumors. This study was aimed to address the complexity and specificity of humoral immune response against a benign human tumor. We assembled a panel of 62 meningioma-expressed antigens that show reactivity with serum antibodies of meningioma pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Comtesse N,Zippel A,Walle S,Monz D,Backes C,Fischer U,Mayer J,Ludwig N,Hildebrandt A,Keller A,Steudel WI,Lenhof HP,Meese E

更新日期：2005-07-05 00:00:00

abstract：:Cyanobacteria are phototrophic prokaryotes that evolved oxygenic photosynthesis ∼2.7 billion y ago and are presently responsible for ∼10% of total global photosynthetic production. To cope with the evolutionary pressure of dropping ambient CO2 concentrations, they evolved a CO2-concentrating mechanism (CCM) to augment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Selim KA,Haase F,Hartmann MD,Hagemann M,Forchhammer K

更新日期：2018-05-22 00:00:00

abstract：:An electrophoretic karyotype of Aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated, with two of the bands migrating as doublets. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brody H,Carbon J

更新日期：1989-08-01 00:00:00