The domain organization of NaeI endonuclease: separation of binding and catalysis.

abstract：:To generate antibodies with different effector functions, B cells undergo Immunoglobulin Heavy Chain (IgH) class switch recombination (CSR). The ligation step of CSR is usually mediated by the classical nonhomologous end-joining (cNHEJ) pathway. In cNHEJ-deficient cells, a remarkable ∼25% of CSR can be achieved by the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang XS,Zhao J,Wu-Baer F,Shao Z,Lee BJ,Cupo OM,Rabadan R,Gautier J,Baer R,Zha S

更新日期：2020-10-13 00:00:00

abstract：:Retinoblastoma-related (RBR) genes inhibit the cell cycle primarily by repressing adenovirus E2 promoter binding factor (E2F) transcription factors, which drive the expression of numerous genes required for DNA synthesis and cell cycle progression. The RBR-E2F pathway is conserved in plants, but cereals such as maize ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sabelli PA,Hoerster G,Lizarraga LE,Brown SW,Gordon-Kamm WJ,Larkins BA

更新日期：2009-03-10 00:00:00

abstract：:In regulating gene expression in mammalian tissues, steroid hormones bind to cytoplasmic receptors and the resulting complex associates with the nucleus. In an attempt to study this nuclear association under more precisely controlled conditions, we examined the binding of receptor-[(3)H]steroid complexes to isolated n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higgins SJ,Rousseau GG,Baxter JD,Tomkins GM

更新日期：1973-12-01 00:00:00

abstract：:Chemical cross-linking mass spectrometry (CXMS) is being increasingly used to study protein assemblies and complex protein interaction networks. Existing CXMS chemical cross-linkers target only Lys, Cys, Glu, and Asp residues, limiting the information measurable. Here we report a "plant-and-cast" cross-linking strateg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang B,Wu H,Schnier PD,Liu Y,Liu J,Wang N,DeGrado WF,Wang L

更新日期：2018-10-30 00:00:00

abstract：:Xenopus laevis oocytes were injected with poly(A)+-mRNA isolated from chorionating follicular epithelium of the domesticated silk moth (Bombyx mori). On two-dimensional gel electrophoresis, the resultant translation products comigrated with authentic, secreted, chorion standards, demonstrating that the frog oocyte sys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bock SC,Tiemeier DC,Mester K,Wu M,Goldsmith MR

更新日期：1982-02-01 00:00:00

abstract：:A recombinant vaccinia virus that directs the synthesis of bacteriophage T7 RNA polymerase provides the basis for the expression of genes that are regulated by T7 promoters in mammalian cells. The T7 transcripts, which account for as much as 30% of the total cytoplasmic RNA at 24 hr after infection, are largely uncapp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elroy-Stein O,Fuerst TR,Moss B

更新日期：1989-08-01 00:00:00

abstract：:A pH-dependent transition between delocalized and trapped mixed valence states of an engineered CuA center in azurin has been investigated by UV-visible absorption and electron paramagnetic resonance spectroscopic techniques. At pH 7.0, the CuA azurin displays a typical delocalized mixed valence dinuclear [Cu(1.5).......

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang HJ,Lu Y

更新日期：2004-08-31 00:00:00

abstract：:Explaining the maintenance of tropical forest diversity under the countervailing forces of drift and competition poses a major challenge to ecological theory. Janzen-Connell effects, in which host-specific natural enemies restrict the recruitment of juveniles near conspecific adults, provide a potential mechanism. Jan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levi T,Barfield M,Barrantes S,Sullivan C,Holt RD,Terborgh J

更新日期：2019-01-08 00:00:00

abstract：:cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan J,Yang X,Wang W,Wood WH 3rd,Becker KG,Gorospe M

更新日期：2002-08-06 00:00:00

abstract：:Fragility is an empirical property that describes how abruptly a glass-forming material solidifies upon supercooling. The degree of fragility carries important implications for the functionality and processability of a material, as well as for our fundamental understanding of the glass transition. However, the microst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ciarella S,Biezemans RA,Janssen LMC

更新日期：2019-12-10 00:00:00

abstract：:The 5'-phosphoinositol phosphatase SHIP negatively regulates signaling pathways triggered by antigen, cytokine and Fc receptors in both lymphocytes and myeloid cells. Mice with germ-line (null) deletion of SHIP develop a myeloproliferative-like syndrome that causes early lethality. Lymphocyte anomalies have been obser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tarasenko T,Kole HK,Chi AW,Mentink-Kane MM,Wynn TA,Bolland S

更新日期：2007-07-03 00:00:00

abstract：:The method of evolutionary stable strategies (ESS), in its current form, is confronted with a difficulty when it tries to explain how some social behaviors initiate their evolution. We show that this difficulty may be removed by changing the assumption made tacitly in game theory (and in ESS) of randomness of meetings...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eshel I,Cavalli-Sforza LL

更新日期：1982-02-01 00:00:00

abstract：:We have identified an adenovirus type 5(Ad5) early gene function located in early region 1 which is required for the production of early cytoplasmic mRNAs corresponding to early regions 2, 3, and 4. Mutant dl312 (lacks the segment between 1.5 and 4.5 map units) grows as well as wild-type virus in 293 cells (Ad5-trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones N,Shenk T

更新日期：1979-08-01 00:00:00

abstract：:Ca(2+)-sensitive kinases are thought to play a role in long-term potentiation (LTP). To test the involvement of Ca2+/calmodulin-dependent kinase II (CaM-K II), truncated, constitutively active form of this kinase was directly injected into CA1 hippocampal pyramidal cells. Inclusion of CaM-K II in the recording pipette...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lledo PM,Hjelmstad GO,Mukherji S,Soderling TR,Malenka RC,Nicoll RA

更新日期：1995-11-21 00:00:00

abstract：:Let T(k) (epsilon)(lambda; t(1),..., t(k)) = rho(X(t) (1))q(epsilon)(X(t) (2) - X(t) (1))... q(epsilon)(X(t(k) ) - X(t(k) )-1), where X(t) is a Brownian motion in R(2), lambda(dx) = rho(x)dx, and q(epsilon) converges to Dirac's delta function as epsilon downward arrow 0. The self-intersection local times of order k ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dynkin EB

更新日期：1986-06-01 00:00:00

abstract：:Since the demonstration that the sequence of a protein encodes its structure, the prediction of structure from sequence remains an outstanding problem that impacts numerous scientific disciplines, including many genome projects. By iteratively fixing secondary structure assignments of residues during Monte Carlo simul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeBartolo J,Colubri A,Jha AK,Fitzgerald JE,Freed KF,Sosnick TR

更新日期：2009-03-10 00:00:00

abstract：:The excitatory neurotransmitter, glutamate, activates N-methyl-d-aspartate (NMDA) receptors to induce long-lasting synaptic changes through alterations in gene expression. It is believed that these long-lasting changes contribute to learning and memory, drug tolerance, and ischemic preconditioning. To identify NMDA-in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong SJ,Li H,Becker KG,Dawson VL,Dawson TM

更新日期：2004-02-17 00:00:00

abstract：:During the late summer to fall of 1987, Caribbean reef corals experienced an intense and widespread discoloration event described as bleaching. Contrary to initial predictions, most bleached corals did not die. However, energy input from zooxanthellae decreased, as estimated from: (i) delta(13)C values, a measure of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter JW,Fitt WK,Spero HJ,Rogers CS,White MW

更新日期：1989-12-01 00:00:00

abstract：:Blood viscosity decreases with shear stress, a property essential for an efficient perfusion of the vascular tree. Shear thinning is intimately related to the dynamics and mutual interactions of RBCs, the major component of blood. Because of the lack of knowledge about the behavior of RBCs under physiological conditio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lanotte L,Mauer J,Mendez S,Fedosov DA,Fromental JM,Claveria V,Nicoud F,Gompper G,Abkarian M

更新日期：2016-11-22 00:00:00

abstract：:Expression of alphavbeta3- or alphavbeta5-integrins and selectins is widespread on blood cells and endothelial cells. Here we report that human tumor cells injected s.c. into mice lacking beta3- or beta3/beta5-integrins or various selectins show enhanced tumor growth compared with growth in control mice. There was inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taverna D,Moher H,Crowley D,Borsig L,Varki A,Hynes RO

更新日期：2004-01-20 00:00:00

abstract：:Although anatomy is often the first step in assigning functions to neural structures, it is not always clear whether architecturally distinct regions of the brain correspond to operational units. Whereas neuroarchitecture remains relatively static, functional connectivity may change almost instantaneously according to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weir PT,Dickinson MH

更新日期：2015-10-06 00:00:00

abstract：:Absorbance changes of C-550 and cytochrome b(559), and fluorescence-yield changes were measured during irradiation of chloroplasts at -196 degrees . The photo-reduction of C-550 proceeded more rapidly than the photo-oxidation of cytochrome b(559), and the fluorescence-yield change had similar kinetics to the cytochrom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler WL

更新日期：1972-11-01 00:00:00

abstract：:Ferritin has a protein shell of 5 X 10(6) Da consisting of 24 subunits of two types, a heavier (H) chain of 21,000 Da and a lighter (L) chain of 19,000 Da. A cDNA clone of the messenger for the L subunit has been isolated from a human monocyte-like leukemia cell line. The clone contains an open reading frame of 522 nu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dörner MH,Salfeld J,Will H,Leibold EA,Vass JK,Munro HN

更新日期：1985-05-01 00:00:00

abstract：:The positive transcription elongation factor b (P-TEFb) is involved in physiological and pathological events including inflammation, cancer, AIDS, and cardiac hypertrophy. The balance between its active and inactive form is tightly controlled to ensure cellular integrity. We report that the transcriptional repressor C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherrier T,Le Douce V,Eilebrecht S,Riclet R,Marban C,Dequiedt F,Goumon Y,Paillart JC,Mericskay M,Parlakian A,Bausero P,Abbas W,Herbein G,Kurdistani SK,Grana X,Van Driessche B,Schwartz C,Candolfi E,Benecke AG,Van Lin

更新日期：2013-07-30 00:00:00

abstract：:In the recently halted HIV type 1 (HIV-1) vaccine STEP trial, individuals that were seropositive for adenovirus serotype 5 (Ad5) showed increased rates of HIV-1 infection on vaccination with an Ad5 vaccine. We propose that this was due to activation and expansion of Ad5-specific mucosal-homing memory CD4 T cells. To t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benlahrech A,Harris J,Meiser A,Papagatsias T,Hornig J,Hayes P,Lieber A,Athanasopoulos T,Bachy V,Csomor E,Daniels R,Fisher K,Gotch F,Seymour L,Logan K,Barbagallo R,Klavinskis L,Dickson G,Patterson S

更新日期：2009-11-24 00:00:00

abstract：:Four synthetic peptides that together constitute the cell attachment domain of fibronectin [Pierschbacher, M.D., Ruoslahti, E., Sundelin, J., Lind, P. & Peterson, P. (1982) J. Biol. Chem. 257, 9593-9597] were constructed and tested for their ability to induce cell attachment and spreading. One of these peptides, consi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pierschbacher M,Hayman EG,Ruoslahti E

更新日期：1983-03-01 00:00:00

abstract：:Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulanovsky L,Bodner M,Trifonov EN,Choder M

更新日期：1986-02-01 00:00:00

abstract：:Supported metal catalysts are extensively used in industrial and environmental applications. To improve their performance, it is crucial to identify the most active sites. This identification is, however, made challenging by the presence of a large number of potential surface structures that complicate such an assignm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang AC,Choksi T,Streibel V,Aljama H,Wrasman CJ,Roling LT,Goodman ED,Thomas D,Bare SR,Sánchez-Carrera RS,Schäfer A,Li Y,Abild-Pedersen F,Cargnello M

更新日期：2020-06-30 00:00:00

abstract：:The signals required for rapid internalization of the bovine cation-dependent mannose 6-phosphate receptor have been localized to two distinct regions of the 67-amino acid cytoplasmic domain. One signal includes phenylalanine 13 and phenylalanine 18, while the other involves tyrosine 45. The former signal is more pote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson KF,Chan W,Kornfeld S

更新日期：1990-12-01 00:00:00

abstract：:We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA. Activation results from the conversion of P1 or P7 RepA dimers to monomers that bind with high af...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wickner S,Skowyra D,Hoskins J,McKenney K

更新日期：1992-11-01 00:00:00