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Global analysis of stress-regulated mRNA turnover by using cDNA arrays.

Abstract：

:cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transcribed (nuclear run-on) RNA, was used to demonstrate the importance of mRNA turnover in regulating gene expression following several conditions of stress.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Fan J,Yang X,Wang W,Wood WH 3rd,Becker KG,Gorospe M

doi

10.1073/pnas.162212399

keywords:

subject

Has Abstract

pub_date

2002-08-06 00:00:00

pages

10611-6

issue

16

eissn

0027-8424

issn

1091-6490

pii

162212399

journal_volume

99

pub_type

杂志文章

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