Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.

abstract：:Solid-state cross-polarization/magic-angle sample-spinning (13)C NMR spectra have been recorded on chlorophyll a-water aggregates, methyl pyrochlorophyllide a, and methyl pyropheophorbide a (derivatives that lack a phytyl chain). Spectra have also been collected under a decoupling regime in which resonances of certain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown CE,Spencer RB,Burger VT,Katz JJ

更新日期：1984-01-01 00:00:00

abstract：:Eleven amino acid positions in the helix-turn-helix of lambda repressor have been mutagenized by using a combinatorial method in which alanine is substituted at each position with a probability of 0.5. Approximately 25% of the 2048 proteins in the resulting binomial library are active, including some variants with as ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gregoret LM,Sauer RT

更新日期：1993-05-01 00:00:00

abstract：:Mechanisms by which the extracellular domain of Notch1 controls Notch1 signaling are not well defined. Here, we show that the O-fucose glycan in the Notch1 ligand-binding domain regulates the strength of Notch1 signaling during embryogenesis, postweaning growth, and T cell development in the mouse. Heterozygotes carry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ge C,Stanley P

更新日期：2008-02-05 00:00:00

abstract：:Cell membrane contact induces the de novo expression of choline O-acetyltransferase (CAT; acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity in cultures of virtually pure neonatal rat dissociated sympathetic neurons. To identify molecular mechanisms underlying membrane-associated CAT induction, the responsi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adler JE,Schleifer LS,Black IB

更新日期：1989-02-01 00:00:00

abstract：:There is not a single pharmaceutical that halts or even slows any neurodegenerative disease. Mounting evidence shows that prions cause many neurodegenerative diseases, and arguably, scrapie and Creutzfeldt-Jakob disease prions represent the best therapeutic targets. We report here that the previously identified 2-amin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berry DB,Lu D,Geva M,Watts JC,Bhardwaj S,Oehler A,Renslo AR,DeArmond SJ,Prusiner SB,Giles K

更新日期：2013-10-29 00:00:00

abstract：:We use alternating-laser excitation to achieve fluorescence-aided molecule sorting (FAMS) and enable simultaneous analysis of biomolecular structure and interactions at the level of single molecules. This was performed by labeling biomolecules with fluorophores that serve as donor-acceptor pairs for Förster resonance ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kapanidis AN,Lee NK,Laurence TA,Doose S,Margeat E,Weiss S

更新日期：2004-06-15 00:00:00

abstract：:Performance on the visual paired-comparison task depends on the integrity of the hippocampal formation in humans, monkeys, and, for an analogous task, in rats. The present study sought additional evidence in healthy volunteers concerning the nature of this task. We found that performance on the visual paired-compariso...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manns JR,Stark CE,Squire LR

更新日期：2000-10-24 00:00:00

abstract：:From 1980 to 1992, a series of influential papers reported on the discovery, genetics, and evolution of a periodic cycling of the interval between Drosophila male courtship song pulses. The molecular mechanisms underlying this periodicity were never described. To reinitiate investigation of this phenomenon, we previou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stern DL,Clemens J,Coen P,Calhoun AJ,Hogenesch JB,Arthur BJ,Murthy M

更新日期：2017-09-12 00:00:00

abstract：:Inhibitory interneurons regulate the responses of cortical circuits. In auditory cortical areas, inhibition from these neurons narrows spectral tuning and shapes response dynamics. Acute disruptions of inhibition expand spectral receptive fields. However, the effects of long-term perturbations of inhibitory circuitry ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seybold BA,Stanco A,Cho KK,Potter GB,Kim C,Sohal VS,Rubenstein JL,Schreiner CE

更新日期：2012-08-21 00:00:00

abstract：:In Trimenia moorei, an extant member of the ancient angiosperm clade Austrobaileyales, we found a remarkable pattern of female gametophyte (egg-producing structure) development that strikingly resembles that of pollen tubes and their intrasexual competition within the maternal pollen tube transmitting tissues of most ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bachelier JB,Friedman WE

更新日期：2011-07-26 00:00:00

abstract：:Mutant I1A cells, lacking IL-1 receptor-associated kinase (IRAK) mRNA and protein, have been used to study the involvement of IRAK in NFkappaB and c-Jun N-terminal kinase (JNK) activation. A series of IRAK deletion constructs were expressed in I1A cells, which were then tested for their ability to respond to IL-1. Bot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Commane M,Jiang Z,Stark GR

更新日期：2001-04-10 00:00:00

abstract：:We recently have shown that mice deficient for the 86-kDa component (Ku80) of the DNA-dependent protein kinase exhibit growth retardation and a profound deficiency in V(D)J (variable, diversity, and joining) recombination. These defects may be related to abnormalities in DNA metabolism that arise from the inability of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nussenzweig A,Sokol K,Burgman P,Li L,Li GC

更新日期：1997-12-09 00:00:00

abstract：:The high frequency, recent origin, and geographic distribution of the CCR5-Delta 32 deletion allele together indicate that it has been intensely selected in Europe. Although the allele confers resistance against HIV-1, HIV has not existed in the human population long enough to account for this selective pressure. The ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galvani AP,Slatkin M

更新日期：2003-12-09 00:00:00

abstract：:Diminished Sonic Hedgehog (Shh) signaling is associated with the most common forebrain defect in humans, holoprosencephaly (HPE), which includes cyclopia, a phenotype also seen in mice and other vertebrates with defective Shh signaling. The secreted protein Shh acts as a crucial factor that patterns the ventral forebr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roessler E,Du YZ,Mullor JL,Casas E,Allen WP,Gillessen-Kaesbach G,Roeder ER,Ming JE,Ruiz i Altaba A,Muenke M

更新日期：2003-11-11 00:00:00

abstract：:Hypothesis directed proteomics offers higher throughput over global analyses. We show that immunoprecipitation (IP)-tandem mass spectrometry (LC-MS/MS) in H929 multiple myeloma (MM) cancer cells led to the discovery of a rare and unexpected BCR-ABL fusion, informing a therapeutic intervention using imatinib (Gleevec)....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breitkopf SB,Yuan M,Pihan GA,Asara JM

更新日期：2012-10-02 00:00:00

abstract：:Recently several attempts to identify oxygen-derived radicals in whole cells by spin trapping and electron spin resonance have been reported by using 5,5-dimethyl-1-pyrroline-N-oxide as the spin trap. In the present study, the feasibility of this method is examined. Chinese hamster V79 cells and human erythrocytes ser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samuni A,Carmichael AJ,Russo A,Mitchell JB,Riesz P

更新日期：1986-10-01 00:00:00

abstract：:Assembly of protein complexes is a key mechanism for achieving spatial and temporal coordination in processes involving many enzymes. Growth of rod-shaped bacteria is a well-studied example requiring such coordination; expansion of the cell wall is thought to involve coordination of the activity of synthetic enzymes w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee TK,Tropini C,Hsin J,Desmarais SM,Ursell TS,Gong E,Gitai Z,Monds RD,Huang KC

更新日期：2014-03-25 00:00:00

abstract：:A leading hypothesis explaining Phanerozoic mass extinctions and associated carbon isotopic anomalies is the emission of greenhouse, other gases, and aerosols caused by eruptions of continental flood basalt provinces. However, the necessary serial relationship between these eruptions, isotopic excursions, and extincti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whiteside JH,Olsen PE,Eglinton T,Brookfield ME,Sambrotto RN

更新日期：2010-04-13 00:00:00

abstract：:The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herlocher ML,Maassab HF,Webster RG

更新日期：1993-07-01 00:00:00

abstract：:The DC and DX subregions of the human major histocompatibility complex (MHC) have been cloned from a cosmid library made from a human B-cell line, Priess. The DC subregion, 48 kilobases, includes the DC alpha and DC beta genes. A second DC-like region, the DX subregion, 35 kilobases, contains the DX alpha gene and a n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okada K,Boss JM,Prentice H,Spies T,Mengler R,Auffray C,Lillie J,Grossberger D,Strominger JL

更新日期：1985-05-01 00:00:00

abstract：:Multiple sclerosis is a chronic disease of the central nervous system (CNS) characterized by inflammation, demyelination, and axonal loss. The immunopathogenesis of demyelination in multiple sclerosis involves an autoantibody response to myelin oligodendrocyte glycoprotein (MOG), a type I transmembrane protein located...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breithaupt C,Schubart A,Zander H,Skerra A,Huber R,Linington C,Jacob U

更新日期：2003-08-05 00:00:00

abstract：:Scanning confocal fluorescence microscopy and multiphoton fluorescence microscopy were used to image the uptake of the protein lysozyme into individual ion exchange chromatography particles in a packed bed in real time. Self-sharpening concentration fronts penetrating into the particles were observed at low salt conce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dziennik SR,Belcher EB,Barker GA,DeBergalis MJ,Fernandez SE,Lenhoff AM

更新日期：2003-01-21 00:00:00

abstract：:The gal operon of Escherichia coli is negatively regulated by repressor binding to bipartite operators separated by 11 helical turns of DNA. Synergistic binding of repressor to separate sites on DNA results in looping, with the intervening DNA as a topologically closed domain containing the two promoters. A closed DNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choy HE,Park SW,Parrack P,Adhya S

更新日期：1995-08-01 00:00:00

abstract：:The ability of polynucleotides or polynucleotide duplexes such as poly(I).poly(C) to induce interferon production is proposed to depend on the existence of certain stable glycosidic orientations. It appears that a slight increase in instability of 1--3 kcal/mole (1 cal = 4.184 J) in the conformational regions near 20 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DL,Miles DW,Eyring H

更新日期：1979-03-01 00:00:00

abstract：:Caseinolytic proteases (ClpPs) are large oligomeric protein complexes that contribute to cell homeostasis as well as virulence regulation in bacteria. Although most organisms possess a single ClpP protein, some organisms encode two or more ClpP isoforms. Here, we elucidated the crystal structures of ClpP1 and ClpP2 fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeiler E,List A,Alte F,Gersch M,Wachtel R,Poreba M,Drag M,Groll M,Sieber SA

更新日期：2013-07-09 00:00:00

abstract：:The low-energy conformations for a series of peptides based on the sequence of the ras P21 protein from position 55 to position 67 have been computed using conformational energy analysis. These sequences differed at position 61 and contained Gln, Pro, Leu, Lys, and Arg at this position. P21 proteins with Gln, Glu, or ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus MR,Brandt-Rauf PW,Carty RP,Lubowsky J,Avitable M,Gibson KD,Scheraga HA

更新日期：1987-12-01 00:00:00

abstract：:In a search for Polo-like kinase 1 (Plk1)-interacting proteins using a yeast two-hybrid system, we have identified histone acetyltransferase binding to the origin recognition complex 1 (Hbo1) as a potential Plk1 target. Here, we show that the interaction between Plk1 and Hbo1 is mitosis-specific and that Plk1 phosphor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu ZQ,Liu X

更新日期：2008-02-12 00:00:00

abstract：:Human mammary epithelial cells secrete and express on their cell surfaces complex mucin glycoproteins (Mr greater than 250,000) that are developmentally regulated, tumor-associated, and highly immunogenic. Studies using monoclonal antibodies directed to these glycoproteins suggest that their molecular structures can v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gendler SJ,Burchell JM,Duhig T,Lamport D,White R,Parker M,Taylor-Papadimitriou J

更新日期：1987-09-01 00:00:00

abstract：:Aggregation of p53 is initiated by first-order processes that generate an aggregation-prone state with parallel pathways of major or partial unfolding. Here, we elaborate the mechanism and explore its consequences, beginning with the core domain and extending to the full-length p53 mutant Y220C. Production of large li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang G,Fersht AR

更新日期：2012-08-21 00:00:00

abstract：:In the filamentous fungus Neurospora crassa, several events in the process of conidiation are influenced by light. Two genes, con-6 and con-10, which were previously shown to be transcriptionally activated during conidiation and by exposure to light, were found to be unexpressed in mycelium maintained in constant dark...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lauter FR,Yanofsky C

更新日期：1993-09-01 00:00:00