Selective NaV1.1 activation rescues Dravet syndrome mice from seizures and premature death.

abstract：:We have used (13C)-1H NMR spectroscopy at 360.13 MHz to resolve the 13C coupled proton resonance of glutamate and lactate in the rat brain in vivo. The time required for the 13C fractional enrichment of the 4-CH2 position of brain glutamate to reach isotopic steady state was determined during a continuous infusion of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothman DL,Behar KL,Hetherington HP,den Hollander JA,Bendall MR,Petroff OA,Shulman RG

更新日期：1985-03-01 00:00:00

abstract：:Human genetic diversity in southern Europe is higher than in other regions of the continent. This difference has been attributed to postglacial expansions, the demic diffusion of agriculture from the Near East, and gene flow from Africa. Using SNP data from 2,099 individuals in 43 populations, we show that estimates o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Botigué LR,Henn BM,Gravel S,Maples BK,Gignoux CR,Corona E,Atzmon G,Burns E,Ostrer H,Flores C,Bertranpetit J,Comas D,Bustamante CD

更新日期：2013-07-16 00:00:00

abstract：:In polymicrobial infections, microbes can interact with both the host immune system and one another through direct contact or the secretion of metabolites, affecting disease progression and treatment options. The thick mucus in the lungs of patients with cystic fibrosis is highly susceptible to polymicrobial infection...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moree WJ,Phelan VV,Wu CH,Bandeira N,Cornett DS,Duggan BM,Dorrestein PC

更新日期：2012-08-21 00:00:00

abstract：:We describe the use of the matrix eigenvalue decomposition (EVD) and pseudoinverse projection and a tensor higher-order EVD (HOEVD) in reconstructing the pathways that compose a cellular system from genome-scale nondirectional networks of correlations among the genes of the system. The EVD formulates a genes x genes n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alter O,Golub GH

更新日期：2005-12-06 00:00:00

abstract：:How do firing patterns in a cortical circuit change when inhibitory neurons are excited? We virally expressed an excitatory designer receptor exclusively activated by a designer drug (Gq-DREADD) in all inhibitory interneuron types of the CA1 region of the hippocampus in the rat. While clozapine N-oxide (CNO) activatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rogers S,Rozman PA,Valero M,Doyle WK,Buzsáki G

更新日期：2021-01-12 00:00:00

abstract：:Fas, a member of the tumor necrosis factor receptor family, can induce apoptosis when activated by Fas ligand binding or anti-Fas antibody crosslinking. Genetic studies have shown that a defect in Fas-mediated apoptosis resulted in abnormal development and function of the immune system in mice. A point mutation in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu K,Niu X,Williams LT

更新日期：1995-12-05 00:00:00

abstract：:Under anesthesia with sodium pentobarbital, the sciatic nerves of rats were transected bilaterally, and a catheter was inserted into the central end of the left renal artery. After an initial flush, an Alzet pump was attached to the catheter, containing various concentrations of glycyl-L-glutamine (Gly-Gln), methylpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koelle GB,Han MS

更新日期：1990-12-01 00:00:00

abstract：:The Pyrococcus furiosus (PF) ornithine carbamoyltransferase (OTCase; EC 2.1.3.3) is an extremely heat-stable enzyme that maintains about 50% of its activity after heat treatment for 60 min at 100 degrees C. To understand the molecular basis of thermostability of this enzyme, we have determined its three-dimensional st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villeret V,Clantin B,Tricot C,Legrain C,Roovers M,Stalon V,Glansdorff N,Van Beeumen J

更新日期：1998-03-17 00:00:00

abstract：:Three-prime repair exonuclease 1 knockout (Trex1-/-) mice suffer from systemic inflammation caused largely by chronic activation of the cyclic GMP-AMP synthase-stimulator of interferon genes-TANK-binding kinase-interferon regulatory factor 3 (cGAS-STING-TBK1-IRF3) signaling pathway. We showed previously that Trex1-def...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasan M,Gonugunta VK,Dobbs N,Ali A,Palchik G,Calvaruso MA,DeBerardinis RJ,Yan N

更新日期：2017-01-24 00:00:00

abstract：:Using animal models of acinar and ductal pancreatic cancer, we investigated the effect of analogs of hypothalamic hormones on tumor growth. In Wistar/Lewis rats bearing the acinar pancreatic tumor DNCP-322, chronic administration of [L-5-Br-Trp8]somatostatin-14 significantly decreased tumor weights and volume. Somatos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Redding TW,Schally AV

更新日期：1984-01-01 00:00:00

abstract：:Recombinant (r) Mycobacterium bovis strains were constructed that secrete biologically active listeriolysin (Hly) fusion protein of Listeria monocytogenes. The r-BCG strains pAT261:Hly or pMV306:Hly expressed plasmid multicopies or chromosomal single copies of the hly gene, respectively. Human and murine macrophage-li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hess J,Miko D,Catic A,Lehmensiek V,Russell DG,Kaufmann SH

更新日期：1998-04-28 00:00:00

abstract：:We have cloned a mutS homolog from Drosophila melanogaster called spellchecker1 (spel1) and have constructed spel1 mutant flies. MutS proteins promote the correction of DNA mismatches and serve important roles in DNA replication, recombination, and repair. The spel1 gene belongs to a subfamily of mutS first characteri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flores C,Engels W

更新日期：1999-03-16 00:00:00

abstract：:Human fibroblast cells treated with a combination of inhibitors of protein and RNA synthesis [cycloheximide and actinomycin D as used to superinduce interferon beta (IFN-beta)] secrete two proteins with molecular masses of 22000 and 27000 kilodaltons (called 22-kDal and 27-kDal) that are precipitable with an antiserum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Content J,De Wit L,Pierard D,Derynck R,De Clercq E,Fiers W

更新日期：1982-05-01 00:00:00

abstract：:We report the use of engineered ribozymes to shuffle exon cassettes in vitro. Specifically, we have designed derivatives of a group II intron that insert into selected sites in the human tissue plasminogen activator (t-PA) mRNA. The insertion reaction links t-PA sequences to the group II intron sequences so that trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mikheeva S,Jarrell KA

更新日期：1996-07-23 00:00:00

abstract：:A model of the photochemical apparatus of photosynthesis, presented previously in a tripartite format, is used in a bipartite format to analyze energy transfer between photosystem II units. The model is used to develop analytical expressions for the photochemical properties of chloroplasts that include a term for the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler WL

更新日期：1980-08-01 00:00:00

abstract：:The periaqueductal gray (PAG) and amygdala are known to be important for defensive responses, and many contemporary fear-conditioning models present the PAG as downstream of the amygdala, directing the appropriate behavior (i.e., freezing or fleeing). However, empirical studies of this circuitry are inconsistent and w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim EJ,Horovitz O,Pellman BA,Tan LM,Li Q,Richter-Levin G,Kim JJ

更新日期：2013-09-03 00:00:00

abstract：:B-DNA becomes unstable under superhelical stress and is able to adopt a wide range of alternative conformations including strand-separated DNA and Z-DNA. Localized sequence-dependent structural transitions are important for the regulation of biological processes such as DNA replication and transcription. To directly p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberstrass FC,Fernandes LE,Bryant Z

更新日期：2012-04-17 00:00:00

abstract：:Blood vessels and nerves often follow parallel trajectories, suggesting that distal targets use common cues that induce vascularization and innervation. Netrins are secreted by the floor plate and attract commissural axons toward the midline of the neural tube. Here, we show that Netrin-1 is also a potent vascular mit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park KW,Crouse D,Lee M,Karnik SK,Sorensen LK,Murphy KJ,Kuo CJ,Li DY

更新日期：2004-11-16 00:00:00

abstract：:Genetic analysis of Parkinson disease (PD) has identified several genes whose mutation causes inherited parkinsonism, as well as risk loci for sporadic PD. PTEN-induced kinase 1 (PINK1) and parkin, linked to autosomal recessive PD, act in a common genetic pathway regulating the autophagic degradation of mitochondria, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivatt RM,Sanchez-Martinez A,Godena VK,Brown S,Ziviani E,Whitworth AJ

更新日期：2014-06-10 00:00:00

abstract：:Vascular endothelial cells (ECs) sense and respond to hemodynamic shear stress, which is critical for circulatory homeostasis and the pathophysiology of vascular diseases. The mechanisms of shear stress mechanotransduction, however, remain elusive. We previously demonstrated a direct role of mitochondria in the purine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto K,Nogimori Y,Imamura H,Ando J

更新日期：2020-12-29 00:00:00

abstract：:Two-electron oxidation of cobaltous octaethylporphyrin [Co(II)(Et)(8)P] yields a stable pi-cation radical [Co(III)(Et)(8)P](2+.), the optical spectrum of which exhibits spectral changes dependent upon the nature of the counterion. Comparison of these spectra with those of Compounds I of horseradish peroxidase and cata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dolphin D,Forman A,Borg DC,Fajer J,Felton RH

更新日期：1971-03-01 00:00:00

abstract：:Prothoracicotropic hormone (PTTH) is the central cerebral neurohormone in insect development. Its release has been believed for decades to be confined to one (or two) critical moments early in each developmental stage at which time it triggers prolonged activation of the prothoracic glands to synthesize and release th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vafopoulou X,Steel CG

更新日期：1996-04-16 00:00:00

abstract：:We recently isolated an RNA polymerase II elongation factor from rat liver nuclei and found it to be homologous to the product of the human ELL gene, a frequent target for translocations in acute myeloid leukemia. To further our understanding of the possible role(s) of ELL in transcriptional regulation and human disea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shilatifard A,Duan DR,Haque D,Florence C,Schubach WH,Conaway JW,Conaway RC

更新日期：1997-04-15 00:00:00

abstract：:The temperature dependence of migration rate and of the thermotactic sensitivity of pseudoplasmodia of Dictyostelium discoideum has been measured. Migration rate increases with temperature to 20 degrees, is temperature insensitive from 20 degrees to 27.5 degrees, and decreases with temperature to 29 degrees, above whi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poff KL,Skokut M

更新日期：1977-05-01 00:00:00

abstract：:Microorganisms must sense their environment and rapidly tune their metabolism to ambient conditions to efficiently use available resources. We have identified a gene encoding a response regulator, NblR, that complements a cyanobacterial mutant unable to degrade its light-harvesting complex (phycobilisome), in response...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwarz R,Grossman AR

更新日期：1998-09-01 00:00:00

abstract：:A gradual buildup of neuronal activity known as the "readiness potential" reliably precedes voluntary self-initiated movements, in the average time locked to movement onset. This buildup is presumed to reflect the final stages of planning and preparation for movement. Here we present a different interpretation of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schurger A,Sitt JD,Dehaene S

更新日期：2012-10-16 00:00:00

abstract：:In that salmonellae have been implicated in an unprecedented array of diseases, sequences found to be specific to this species are often thought to be involved in the virulence attributes not seen in other enteric bacteria. To identify the molecular, genetic, and phenotypic characteristics that differentiate bacterial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Groisman EA,Sturmoski MA,Solomon FR,Lin R,Ochman H

更新日期：1993-02-01 00:00:00

abstract：:Cell-cycle progression is mediated by a co-ordinated interaction between cyclin-dependent kinases and their target proteins including the pRB and E2F/DP-1 complexes. Immunoneutralization and antisense experiments have established that the abundance of cyclin D1, a regulatory subunit of the cyclin-dependent kinases, ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe G,Howe A,Lee RJ,Albanese C,Shu IW,Karnezis AN,Zon L,Kyriakis J,Rundell K,Pestell RG

更新日期：1996-11-12 00:00:00

abstract：:Rabbit articular chondrocytes synthesize type II collagen [3alpha(1)(II)] in vivo and type I collagen [2alpha(1)(I).alpha(2)] in monolayer cultures. In suspension culture the nature of phenotype depends on extracellular Ca(2+). The relationship of Ca(2+) and 3':5'-cyclic AMP (cAMP) in regulation of collagen synthesis ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deshmukh K,Sawyer BD

更新日期：1977-09-01 00:00:00

abstract：:DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dohet C,Wagner R,Radman M

更新日期：1986-05-01 00:00:00