当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Use of engineered ribozymes to catalyze chimeric gene assembly.

Use of engineered ribozymes to catalyze chimeric gene assembly.

Abstract：

:We report the use of engineered ribozymes to shuffle exon cassettes in vitro. Specifically, we have designed derivatives of a group II intron that insert into selected sites in the human tissue plasminogen activator (t-PA) mRNA. The insertion reaction links t-PA sequences to the group II intron sequences so that trans-splicing reactions catalyzed by the intron can be employed to shuffle the t-PA sequences. We expect these results to be generalizable, so that similar ribozymes can be designed to target any desired 13 nucleotide sequence. In principle, the reactions we describe here should be able to link any RNA molecule to any other RNA molecule at any selected point.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Mikheeva S,Jarrell KA

doi

10.1073/pnas.93.15.7486

subject

Has Abstract

pub_date

1996-07-23 00:00:00

pages

7486-90

issue

15

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Metabolism of Alzheimer beta-amyloid precursor protein: regulation by protein kinase A in intact cells and in a cell-free system.

    abstract：:Various compounds that affect signal transduction regulate the relative utilization of alternative processing pathways for the beta-amyloid precursor protein (beta APP) in intact cells, increasing the production of nonamyloidogenic soluble beta APP (s beta APP) and decreasing that of amyloidogenic beta-amyloid peptide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.9.4081

    authors： Xu H,Sweeney D,Greengard P,Gandy S

    更新日期：1996-04-30 00:00:00

  • Plants and animals share functionally common bacterial virulence factors.

    abstract：:By exploiting the ability of Pseudomonas aeruginosa to infect a variety of vertebrate and nonvertebrate hosts, we have developed model systems that use plants and nematodes as adjuncts to mammalian models to help elucidate the molecular basis of P. aeruginosa pathogenesis. Our studies reveal a remarkable degree of con...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.97.16.8815

    authors： Rahme LG,Ausubel FM,Cao H,Drenkard E,Goumnerov BC,Lau GW,Mahajan-Miklos S,Plotnikova J,Tan MW,Tsongalis J,Walendziewicz CL,Tompkins RG

    更新日期：2000-08-01 00:00:00

  • Protein kinase N1, a cell inhibitor of Akt kinase, has a central role in quality control of germinal center formation.

    abstract：:Germinal centers (GCs) are specialized microenvironments in secondary lymphoid organs where high-affinity antibody-producing B cells are selected based on B-cell antigen receptor (BCR) signal strength. BCR signaling required for normal GC selection is uncertain. We have found that protein kinase N1 (PKN1, also known a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1218925110

    authors： Yasui T,Sakakibara-Yada K,Nishimura T,Morita K,Tada S,Mosialos G,Kieff E,Kikutani H

    更新日期：2012-12-18 00:00:00

  • Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers.

    abstract：:We have made direct, quantitative measurements of the lateral motion and age-dependent distribution of acetylcholine receptors (AChR) on the surface of rat myotubes in primary culture. AChR were fluorescently marked with tetramethylrhodamine-labeled alpha-bungarotoxin and AChR lateral motion was measured by the fluore...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.12.4594

    authors： Axelrod D,Ravdin P,Koppel DE,Schlessinger J,Webb WW,Elson EL,Podleski TR

    更新日期：1976-12-01 00:00:00

  • Sexually dimorphic effects of forkhead box a2 (FOXA2) and uterine glands on decidualization and fetoplacental development.

    abstract：:Glands of the uterus are essential for pregnancy establishment. Forkhead box A2 (FOXA2) is expressed specifically in the glands of the uterus and a critical regulator of glandular epithelium (GE) differentiation, development, and function. Mice with a conditional deletion of FOXA2 in the adult uterus, created using th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2014272117

    authors： Dhakal P,Kelleher AM,Behura SK,Spencer TE

    更新日期：2020-09-22 00:00:00

  • Mutational landscape of primary, metastatic, and recurrent ovarian cancer reveals c-MYC gains as potential target for BET inhibitors.

    abstract：:Ovarian cancer remains the most lethal gynecologic malignancy. We analyzed the mutational landscape of 64 primary, 41 metastatic, and 17 recurrent fresh-frozen tumors from 77 patients along with matched normal DNA, by whole-exome sequencing (WES). We also sequenced 13 pairs of synchronous bilateral ovarian cancer (SBO...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1814027116

    authors： Li C,Bonazzoli E,Bellone S,Choi J,Dong W,Menderes G,Altwerger G,Han C,Manzano A,Bianchi A,Pettinella F,Manara P,Lopez S,Yadav G,Riccio F,Zammataro L,Zeybek B,Yang-Hartwich Y,Buza N,Hui P,Wong S,Ravaggi A,Bigno

    更新日期：2019-01-08 00:00:00

  • RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells.

    abstract：:Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemical synthesis of si...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.092143499

    authors： Yu JY,DeRuiter SL,Turner DL

    更新日期：2002-04-30 00:00:00

  • Efficient genetically controlled formation of antibody to a synthetic antigen [poly(LTyr, LGlu)-poly(DLAla)- -poly(LLys)] covalently bound to a synthetic adjuvant (N-acetylmuramyl-L-alanyl-D-isoglutamine).

    abstract：:The synthetic polypeptide antigen poly(LTyr, LGlu)-poly(DLAl)- -poly(LLys)[T,G)-A- -L] was covalently linked to N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), which is the minimal adjuvant-active structure that can substitute for Mycobacteria in complete Freund's adjuvant. When injected in aqueous solution into mice, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.8.4933

    authors： Mozes E,Sela M,Chedid L

    更新日期：1980-08-01 00:00:00

  • Role of the intestinal tight junction modulator zonulin in the pathogenesis of type I diabetes in BB diabetic-prone rats.

    abstract：:Increased intestinal permeability has been observed in numerous human autoimmune diseases, including type-1 diabetes (T1D) and its' animal model, the BB-wor diabetic prone rat. We have recently described zonulin, a protein that regulates intercellular tight junctions. The objective of this study was to establish wheth...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0500178102

    authors： Watts T,Berti I,Sapone A,Gerarduzzi T,Not T,Zielke R,Fasano A

    更新日期：2005-02-22 00:00:00

  • A natural classification of the basic helix-loop-helix class of transcription factors.

    abstract：:A natural (evolutionary) classification is provided for 242 basic helix-loop-helix (bHLH) motif-containing proteins. Phylogenetic analyses of amino acid sequences describe the patterns of evolutionary change within the motif and delimit evolutionary lineages. These evolutionary lineages represent well known functional...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.10.5172

    authors： Atchley WR,Fitch WM

    更新日期：1997-05-13 00:00:00

  • Picosecond primary structural transition of the heme is retarded after nitric oxide binding to heme proteins.

    abstract：:We investigated the ultrafast structural transitions of the heme induced by nitric oxide (NO) binding for several heme proteins by subpicosecond time-resolved resonance Raman and femtosecond transient absorption spectroscopy. We probed the heme iron motion by the evolution of the iron-histidine Raman band intensity af...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912938107

    authors： Kruglik SG,Yoo BK,Franzen S,Vos MH,Martin JL,Negrerie M

    更新日期：2010-08-03 00:00:00

  • Larval helpers and age polyethism in ambrosia beetles.

    abstract：:Division of labor among the workers of insect societies is a conspicuous feature of their biology. Social tasks are commonly shared among age groups but not between larvae and adults with completely different morphologies, as in bees, wasps, ants, and beetles (i.e., Holometabola). A unique yet hardly studied holometab...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1107758108

    authors： Biedermann PH,Taborsky M

    更新日期：2011-10-11 00:00:00

  • Patterns of coevolving amino acids unveil structural and dynamical domains.

    abstract：:Patterns of interacting amino acids are so preserved within protein families that the sole analysis of evolutionary comutations can identify pairs of contacting residues. It is also known that evolution conserves functional dynamics, i.e., the concerted motion or displacement of large protein regions or domains. Is it...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1712021114

    authors： Granata D,Ponzoni L,Micheletti C,Carnevale V

    更新日期：2017-12-12 00:00:00

  • Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2.

    abstract：:alpha-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors undergo constitutive and ligand-induced internalization that requires dynamin and the clathrin adaptor complex AP-2. We report here that an atypical basic motif within the cytoplasmic tails of AMPA-type glutamate receptors directl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611170104

    authors： Kastning K,Kukhtina V,Kittler JT,Chen G,Pechstein A,Enders S,Lee SH,Sheng M,Yan Z,Haucke V

    更新日期：2007-02-20 00:00:00

  • Inhibition of the activation of Hageman factor (factor XII) by human vascular endothelial cell culture supernates.

    abstract：:The supernatant fluid (conditioned medium) of cultured human vascular endothelial cells inhibits activation of Hageman factor (factor XII), whether by ellagic acid, bovine brain sulfatides, or bismuth subgallate; inhibition appears to be a property of one or more proteins in the culture supernates. This phenomenon may...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.23.10740

    authors： Ratnoff OD,Everson B,Embury P,Ziats NP,Anderson JM,Emanuelson MM,Malemud CJ

    更新日期：1991-12-01 00:00:00

  • Insulin growth factor signaling is regulated by microRNA-486, an underexpressed microRNA in lung cancer.

    abstract：:MicroRNAs (miRNAs) are small 19- to 24-nt noncoding RNAs that have the capacity to regulate fundamental biological processes essential for cancer initiation and progression. In cancer, miRNAs may function as oncogenes or tumor suppressors. Here, we conducted global profiling for miRNAs in a cohort of stage 1 nonsmall ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1307107110

    authors： Peng Y,Dai Y,Hitchcock C,Yang X,Kassis ES,Liu L,Luo Z,Sun HL,Cui R,Wei H,Kim T,Lee TJ,Jeon YJ,Nuovo GJ,Volinia S,He Q,Yu J,Nana-Sinkam P,Croce CM

    更新日期：2013-09-10 00:00:00

  • Antibodies in human serum that precipitate ribonuclease P.

    abstract：:Sera from certain patients with systemic lupus erythematosus (SLE) and related rheumatic diseases contain antibodies that selectively deplete extracts of HeLa cells of RNase P activity. Most of the sera that recognize RNase P, an endoribonuclease with an essential RNA subunit, also contain antibodies against another s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.15.5483

    authors： Gold HA,Craft J,Hardin JA,Bartkiewicz M,Altman S

    更新日期：1988-08-01 00:00:00

  • Resistance-associated point mutations in insecticide-insensitive acetylcholinesterase.

    abstract：:Extensive utilization of pesticides against insects provides us with a good model for studying the adaptation of a eukaryotic genome to a strong selective pressure. One mechanism of resistance is the alteration of acetylcholinesterase (EC 3.1.1.7), the molecular target for organophosphates and carbamates. Here, we rep...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.13.5922

    authors： Mutero A,Pralavorio M,Bride JM,Fournier D

    更新日期：1994-06-21 00:00:00

  • Extraordinary intelligence and the care of infants.

    abstract：:We present evidence that pressures for early childcare may have been one of the driving factors of human evolution. We show through an evolutionary model that runaway selection for high intelligence may occur when (i) altricial neonates require intelligent parents, (ii) intelligent parents must have large brains, and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 评论,杂志文章

    doi：10.1073/pnas.1506752113

    authors： Piantadosi ST,Kidd C

    更新日期：2016-06-21 00:00:00

  • Gravitational instabilities in binary granular materials.

    abstract：:The motion and mixing of granular media are observed in several contexts in nature, often displaying striking similarities to liquids. Granular dynamics occur in geological phenomena and also enable technologies ranging from pharmaceuticals production to carbon capture. Here, we report the discovery of a family of gra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1820820116

    authors： McLaren CP,Kovar TM,Penn A,Müller CR,Boyce CM

    更新日期：2019-05-07 00:00:00

  • Sequence-independent upstream DNA-alphaCTD interactions strongly stimulate Escherichia coli RNA polymerase-lacUV5 promoter association.

    abstract：:The C-terminal domains of the two alpha-subunits (alphaCTD) in Escherichia coli RNA polymerase (RNAP) recognize specific sequences called UP elements in some promoters. These interactions can increase transcription dramatically. Previously, effects of upstream DNA-alphaCTD interactions on transcription were quantified...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0405814102

    authors： Ross W,Gourse RL

    更新日期：2005-01-11 00:00:00

  • DNA damage related to increased hydrogen peroxide generation by hypolipidemic drug-induced liver peroxisomes.

    abstract：:Several hypolipidemic drugs and certain industrial plasticizers induce proliferation of peroxisomes, enhance the activity of peroxisome-associated beta-oxidation of fatty acids, and produce hepatocellular carcinomas in the livers of rodents. Because these chemicals themselves are not mutagens and do not covalently mod...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.24.7827

    authors： Fahl WE,Lalwani ND,Watanabe T,Goel SK,Reddy JK

    更新日期：1984-12-01 00:00:00

  • BCR/ABL inhibition by an escort/phosphatase fusion protein.

    abstract：:Cellular transformation by the BCR/ABL oncogene depends on the ABL-encoded tyrosine kinase activity. To block BCR/ABL function, we created a unique tyrosine phosphatase by fusing the catalytic domain of SHP1 (SHP1c) to the ABL binding domain (ABD) of RIN1, an established binding partner and substrate for c-ABL and BCR...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.210253497

    authors： Lim YM,Wong S,Lau G,Witte ON,Colicelli J

    更新日期：2000-10-24 00:00:00

  • Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity.

    abstract：:The proteasome regulates cellular processes as diverse as cell cycle progression and NF-kappaB activation. In this study, we show that the potent antitumor natural product epoxomicin specifically targets the proteasome. Utilizing biotinylated-epoxomicin as a molecular probe, we demonstrate that epoxomicin covalently b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.18.10403

    authors： Meng L,Mohan R,Kwok BH,Elofsson M,Sin N,Crews CM

    更新日期：1999-08-31 00:00:00

  • Chromatin landscape dynamics of the Il4-Il13 locus during T helper 1 and 2 development.

    abstract：:Il4 and Il13 encode the canonical T helper 2 (TH2) cytokines responsible both for promoting immune responses against extracellular pathogens and, when misregulated, causing allergic and autoimmune disease. The expression potential of these genes undergoes developmentally programmed repression and enhancement during co...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0403334101

    authors： Baguet A,Bix M

    更新日期：2004-08-03 00:00:00

  • Intragenic alternative splicing coordination is essential for Caenorhabditis elegans slo-1 gene function.

    abstract：:Alternative splicing is critical for diversifying eukaryotic proteomes, but the rules governing and coordinating splicing events among multiple alternate splice sites within individual genes are not well understood. We developed a quantitative PCR-based strategy to quantify the expression of the 12 transcripts encoded...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1116712108

    authors： Glauser DA,Johnson BE,Aldrich RW,Goodman MB

    更新日期：2011-12-20 00:00:00

  • Transcription of oxygen-regulated photosynthetic genes requires DNA gyrase in Rhodobacter capsulatus.

    abstract：:The regulation of the photosynthetic genes by DNA supercoiling in Rhodobacter capsulatus has been studied by using gyrase inhibitors in vivo and by measurement of mRNA levels of more than a dozen genes. The results demonstrate that the levels of mRNA for light-harvesting (I, II) and reaction center (L, M, H) proteins,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.12.4209

    authors： Zhu YS,Hearst JE

    更新日期：1988-06-01 00:00:00

  • An original adaptation of photosynthesis in the marine green alga Ostreococcus.

    abstract：:Adaptation of photosynthesis in marine environment has been examined in two strains of the green, picoeukaryote Ostreococcus: OTH95, a surface/high-light strain, and RCC809, a deep-sea/low-light strain. Differences between the two strains include changes in the light-harvesting capacity, which is lower in OTH95, and i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0802762105

    authors： Cardol P,Bailleul B,Rappaport F,Derelle E,Béal D,Breyton C,Bailey S,Wollman FA,Grossman A,Moreau H,Finazzi G

    更新日期：2008-06-03 00:00:00

  • Transglutaminase 2 is essential for adherence of Porphyromonas gingivalis to host cells.

    abstract：:Porphyromonas gingivalis is the major causative agent of periodontitis, and it may also be involved in the development of systemic diseases (atherosclerosis, rheumatoid arthritis). P. gingivalis is found on and within oral and gingival epithelial cells following binding to surface components of host cells, which serve...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1402740111

    authors： Boisvert H,Lorand L,Duncan MJ

    更新日期：2014-04-08 00:00:00

  • Liver uptake of chylomicron remnants with high and low apoprotein E:C ratios.

    abstract：:The ability of the isolated perfused rat liver to differentiate between chylomicrons and remnants with either high or low apoprotein E:C ratios was investigated. Remnants were prepared in hepatectomized rats injected with chylomicrons double-labeled with [3H]cholesterol and 14C-labeled fatty acids. By densitometric sc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.18.5863

    authors： Borensztajn J,Kotlar TJ

    更新日期：1984-09-01 00:00:00