Abstract:
:We have used (13C)-1H NMR spectroscopy at 360.13 MHz to resolve the 13C coupled proton resonance of glutamate and lactate in the rat brain in vivo. The time required for the 13C fractional enrichment of the 4-CH2 position of brain glutamate to reach isotopic steady state was determined during a continuous infusion of D-[1-13C]glucose. Under conditions of ischemia, measurements made of the 3-CH3 of lactate in (13C)-1H NMR spectra revealed the relative contribution of brain glucose and glycogen to lactate formation. (13C)-1H NMR was 11 times more sensitive than 13C NMR for the detection of 13C in the 3-CH3 position of lactate and 6 times more sensitive for the detection of 13C in the 4-CH2 of glutamate under similar in vivo conditions.
journal_name
Proc Natl Acad Sci U S Aauthors
Rothman DL,Behar KL,Hetherington HP,den Hollander JA,Bendall MR,Petroff OA,Shulman RGdoi
10.1073/pnas.82.6.1633subject
Has Abstractpub_date
1985-03-01 00:00:00pages
1633-7issue
6eissn
0027-8424issn
1091-6490journal_volume
82pub_type
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