当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Synthesis of collagen by chondrocytes in suspension culture: modulation by calcium, 3':5'-cyclic AMP, and prostaglandins.

Synthesis of collagen by chondrocytes in suspension culture: modulation by calcium, 3':5'-cyclic AMP, and prostaglandins.

Abstract：

:Rabbit articular chondrocytes synthesize type II collagen [3alpha(1)(II)] in vivo and type I collagen [2alpha(1)(I).alpha(2)] in monolayer cultures. In suspension culture the nature of phenotype depends on extracellular Ca(2+). The relationship of Ca(2+) and 3':5'-cyclic AMP (cAMP) in regulation of collagen synthesis has been investigated. In suspension culture, cAMP levels of chondrocytes increase by 2- to 3-fold and then reach basal values regardless of the presence or absence of extracellular Ca(2+). The cells, however, synthesize primarily type II collagen in the absence of CaCl(2) in the medium and type I collagen in medium containing 1.8 mM CaCl(2). If CaCl(2) is added when intracellular cAMP levels are low, the phenotype is type I collagen. These observations minimize the role of cAMP as a second messenger in the chondrocyte culture system. Increasing endogenous cAMP with a phosphodiesterase inhibitor or adding exogenous dibutyryl-cAMP leads the cells to synthesize type I collagen, although this effect is significantly less pronounced if the medium contains ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA). Increased concentrations of cAMP may mobilize the intracellular calcium pools and activate the cells to switch their phenotypic expression. Prostaglandins E(2) and F(2)alpha, thought to be involved in rheumatoid arthritis and bone resorption, have no significant effect on cAMP content of chondrocytes and alter their collagen phenotype to a small extent.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Deshmukh K,Sawyer BD

doi

10.1073/pnas.74.9.3864

subject

Has Abstract

pub_date

1977-09-01 00:00:00

pages

3864-8

issue

9

eissn

0027-8424

issn

1091-6490

journal_volume

74

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • DNA replication meets genetic exchange: chromosomal damage and its repair by homologous recombination.

    abstract：:Proceedings of the National Academy of Sciences Colloquium on the roles of homologous recombination in DNA replication are summarized. Current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting DNA replication ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.151260698

    authors： Kuzminov A

    更新日期：2001-07-17 00:00:00

  • Genetic absolute dating based on microsatellites and the origin of modern humans.

    abstract：:We introduce a new genetic distance for microsatellite loci, incorporating features of the stepwise mutation model, and test its performance on microsatellite polymorphisms in humans, chimpanzees, and gorillas. We find that it performs well in determining the relations among the primates, but less well than other dist...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.15.6723

    authors： Goldstein DB,Ruiz Linares A,Cavalli-Sforza LL,Feldman MW

    更新日期：1995-07-18 00:00:00

  • Structure, domain organization, and different conformational states of stem cell factor-induced intact KIT dimers.

    abstract：:Using electron microscopy and fitting of crystal structures, we present the 3D reconstruction of ligand-induced dimers of intact receptor tyrosine kinase, KIT. We observe that KIT protomers form close contacts throughout the entire structure of ligand-bound receptor dimers, and that the dimeric receptors adopt multipl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1323254111

    authors： Opatowsky Y,Lax I,Tomé F,Bleichert F,Unger VM,Schlessinger J

    更新日期：2014-02-04 00:00:00

  • Revealing the three-dimensional structure of liquids using four-point correlation functions.

    abstract：:Disordered systems like liquids, gels, glasses, or granular materials are not only ubiquitous in daily life and in industrial applications, but they are also crucial for the mechanical stability of cells or the transport of chemical and biological agents in living organisms. Despite the importance of these systems, th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2005638117

    authors： Zhang Z,Kob W

    更新日期：2020-06-23 00:00:00

  • Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1.

    abstract：:The Arabidopsis thaliana disease resistance genes RPS2 and RPM1 belong to a class of plant disease resistance genes that encode proteins that contain an N-terminal tripartite nucleotide binding site (NBS) and a C-terminal tandem array of leucine-rich repeats. RPS2 and RPM1 confer resistance to strains of the bacterial...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.26.15497

    authors： Leister RT,Ausubel FM,Katagiri F

    更新日期：1996-12-24 00:00:00

  • Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach.

    abstract：:The study of the metabolome presents numerous challenges, first among them being the cataloging of its constituents. A step in this direction will be the development of tools to identify metabolites that share common structural features. The importance of sulfated molecules in cell-cell communication motivated us to d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.252514899

    authors： Mougous JD,Leavell MD,Senaratne RH,Leigh CD,Williams SJ,Riley LW,Leary JA,Bertozzi CR

    更新日期：2002-12-24 00:00:00

  • Association of titin and myosin heavy chain in developing skeletal muscle.

    abstract：:To understand molecular interactions that organize developing myofibrils, we examined the biosynthesis and interaction of titin and myosin heavy chain in cultures of developing muscle. Use of pulse-labeling, immunoprecipitation, and a reversible cross-linking procedure demonstrates that within minutes of synthesis, ti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.16.7496

    authors： Isaacs WB,Kim IS,Struve A,Fulton AB

    更新日期：1992-08-15 00:00:00

  • Science, health, and cultural literacy in a rapidly changing communications landscape.

    abstract：:There is a gap between how many scientists communicate and how most people understand and interpret messages. This article argues that the extensive science communications literature needs to be joined by the health literacy literature and anthropological work on cultural variations in hearing and understanding messag...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1807218116

    authors： Scrimshaw SC

    更新日期：2019-04-16 00:00:00

  • Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.

    abstract：:A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of the isolated gene r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.7.1931

    authors： Takahashi Y,Kato K,Hayashizaki Y,Wakabayashi T,Ohtsuka E,Matsuki S,Ikehara M,Matsubara K

    更新日期：1985-04-01 00:00:00

  • Centromere-encoded RNAs are integral components of the maize kinetochore.

    abstract：:RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA-chromatin interactions to the centromere core. The data indicate that maize centromeric retrotransposons (CRMs) and satellite repeats (CentC) a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0407154101

    authors： Topp CN,Zhong CX,Dawe RK

    更新日期：2004-11-09 00:00:00

  • Kinetic analysis of estrogen receptor/ligand interactions.

    abstract：:Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the association and di...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.142288199

    authors： Rich RL,Hoth LR,Geoghegan KF,Brown TA,LeMotte PK,Simons SP,Hensley P,Myszka DG

    更新日期：2002-06-25 00:00:00

  • Prestimulus influences on auditory perception from sensory representations and decision processes.

    abstract：:The qualities of perception depend not only on the sensory inputs but also on the brain state before stimulus presentation. Although the collective evidence from neuroimaging studies for a relation between prestimulus state and perception is strong, the interpretation in the context of sensory computations or decision...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524087113

    authors： Kayser SJ,McNair SW,Kayser C

    更新日期：2016-04-26 00:00:00

  • HIV-1 envelope trimer elicits more potent neutralizing antibody responses than monomeric gp120.

    abstract：:HIV-1 envelope glycoprotein is the primary target for HIV-1-specific antibodies. The native HIV-1 envelope spike on the virion surface is a trimer, but trimeric gp140 and monomeric gp120 currently are believed to induce comparable immune responses. Indeed, most studies on the immunogenicity of HIV-1 envelope oligomers...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1204533109

    authors： Kovacs JM,Nkolola JP,Peng H,Cheung A,Perry J,Miller CA,Seaman MS,Barouch DH,Chen B

    更新日期：2012-07-24 00:00:00

  • Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods.

    abstract：:Sodium/hydrogen exchangers (NHEs) are ubiquitous ion transporters that serve multiple cell functions. We have studied two mammalian isoforms, NHE1 (ubiquitous) and NHE3 (epithelial-specific), by measuring extracellular proton (H+) gradients during whole-cell patch clamp with perfusion of the cell interior. Maximal Na(...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0403930101

    authors： Fuster D,Moe OW,Hilgemann DW

    更新日期：2004-07-13 00:00:00

  • Reconstitution of a deoxyribonuclease I-sensitive structure on active genes.

    abstract：:Chicken erythrocyte nuclei have been labeled in the active regions of the chromosome by using the nick translation reaction. In this procedure, accessible areas of the genome are preferentially nicked by the action of pancreatic DNase I and subsequently labeled by using DNA polymerase I from Escherichia coli. These nu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.4.1787

    authors： Gazit B,Panet A,Cedar H

    更新日期：1980-04-01 00:00:00

  • Quantifying uncertainty in climate change science through empirical information theory.

    abstract：:Quantifying the uncertainty for the present climate and the predictions of climate change in the suite of imperfect Atmosphere Ocean Science (AOS) computer models is a central issue in climate change science. Here, a systematic approach to these issues with firm mathematical underpinning is developed through empirical...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1007009107

    authors： Majda AJ,Gershgorin B

    更新日期：2010-08-24 00:00:00

  • Waves and stimulus-modulated dynamics in an oscillating olfactory network.

    abstract：:The temporal dynamics of electrical activity in an olfactory organ, the procerebral lobe of the terrestrial mollusc Limax maximus, is studied. The lobe exhibits intrinsic oscillations in its field potential. Intracellular recordings show that the lobe contains two classes of neurons, both with activity phase-locked to...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.2.669

    authors： Delaney KR,Gelperin A,Fee MS,Flores JA,Gervais R,Tank DW,Kleinfeld D

    更新日期：1994-01-18 00:00:00

  • Verifying Larché-Cahn elasticity, a milestone of 20th-century thermodynamics.

    abstract：:Many materials phenomena are governed by the interaction between chemistry and mechanics. However, it was only in the second half of the 20th century that the theory of open system elasticity by Francis Larché and John W. Cahn concatenated the fields of solid mechanics and alloy chemistry. As the theory's central mate...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1809355115

    authors： Shi S,Markmann J,Weissmüller J

    更新日期：2018-10-23 00:00:00

  • Analysis of barotactic and chemotactic guidance cues on directional decision-making of Dictyostelium discoideum cells in confined environments.

    abstract：:Neutrophils and dendritic cells when migrating in confined environments have been shown to actuate a directional choice toward paths of least hydraulic resistance (barotaxis), in some cases overriding chemotactic responses. Here, we investigate whether this barotactic response is conserved in the more primitive model ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2000686117

    authors： Belotti Y,McGloin D,Weijer CJ

    更新日期：2020-10-13 00:00:00

  • High-order fractal states in graphene superlattices.

    abstract：:Graphene superlattices were shown to exhibit high-temperature quantum oscillations due to periodic emergence of delocalized Bloch states in high magnetic fields such that unit fractions of the flux quantum pierce a superlattice unit cell. Under these conditions, semiclassical electron trajectories become straight agai...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1804572115

    authors： Krishna Kumar R,Mishchenko A,Chen X,Pezzini S,Auton GH,Ponomarenko LA,Zeitler U,Eaves L,Fal'ko VI,Geim AK

    更新日期：2018-05-15 00:00:00

  • Identification of a receptor required for the anti-inflammatory activity of IVIG.

    abstract：:The anti-inflammatory activity of intravenous Ig (IVIG) results from a minor population of the pooled IgG molecules that contains terminal alpha2,6-sialic acid linkages on their Fc-linked glycans. These anti-inflammatory properties can be recapitulated with a fully recombinant preparation of appropriately sialylated I...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0810163105

    authors： Anthony RM,Wermeling F,Karlsson MC,Ravetch JV

    更新日期：2008-12-16 00:00:00

  • Sequencing newly replicated DNA reveals widespread plasticity in human replication timing.

    abstract：:Faithful transmission of genetic material to daughter cells involves a characteristic temporal order of DNA replication, which may play a significant role in the inheritance of epigenetic states. We developed a genome-scale approach--Repli Seq--to map temporally ordered replicating DNA using massively parallel sequenc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912402107

    authors： Hansen RS,Thomas S,Sandstrom R,Canfield TK,Thurman RE,Weaver M,Dorschner MO,Gartler SM,Stamatoyannopoulos JA

    更新日期：2010-01-05 00:00:00

  • Orthogonal arrangement of rhythm-generating microcircuits in the hippocampus.

    abstract：:As a structure involved in learning and memory, the hippocampus functions as a network. The functional differentiation along the longitudinal axis of the hippocampus is poorly demarcated in comparison with the transverse axis. Using patch clamp recordings in conjunction with post hoc anatomy, we have examined the patt...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0506259102

    authors： Gloveli T,Dugladze T,Rotstein HG,Traub RD,Monyer H,Heinemann U,Whittington MA,Kopell NJ

    更新日期：2005-09-13 00:00:00

  • Diversity of Holocene life forms in fossil glacier ice.

    abstract：:Studies of biotic remains of polar ice caps have been limited to morphological identification of plant pollen and spores. By using sensitive molecular techniques, we now demonstrate a much greater range of detectable organisms; from 2000- and 4000-year-old ice-core samples, we obtained and characterized 120 clones tha...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.14.8017

    authors： Willerslev E,Hansen AJ,Christensen B,Steffensen JP,Arctander P

    更新日期：1999-07-06 00:00:00

  • Understanding missense mutations in the BRCA1 gene: an evolutionary approach.

    abstract：:The role of missense changes in BRCA1 in breast cancer susceptibility has been difficult to establish. We used comparative evolutionary methods to identify potential functionally important amino acid sites in exon 11 and missense changes likely to disrupt gene function, aligning sequences from 57 eutherian mammals and...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0237285100

    authors： Fleming MA,Potter JD,Ramirez CJ,Ostrander GK,Ostrander EA

    更新日期：2003-02-04 00:00:00

  • Inherent noise can facilitate coherence in collective swarm motion.

    abstract：:Among the most striking aspects of the movement of many animal groups are their sudden coherent changes in direction. Recent observations of locusts and starlings have shown that this directional switching is an intrinsic property of their motion. Similar direction switches are seen in self-propelled particle and othe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0811195106

    authors： Yates CA,Erban R,Escudero C,Couzin ID,Buhl J,Kevrekidis IG,Maini PK,Sumpter DJ

    更新日期：2009-04-07 00:00:00

  • Protection from mitochondrial complex II inhibition in vitro and in vivo by Nrf2-mediated transcription.

    abstract：:Complex II inhibitors 3-nitropropionic acid (3NP) and malonate cause striatal damage reminiscent of Huntington's disease and have been shown to involve oxidative stress in their pathogenesis. Because nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent transcriptional activation by means of the antioxidant res...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0408487101

    authors： Calkins MJ,Jakel RJ,Johnson DA,Chan K,Kan YW,Johnson JA

    更新日期：2005-01-04 00:00:00

  • Induction of B-cell differentiation antigens in interferon- or phorbol ester-treated Daudi cells is impaired by inhibitors of ADP-ribosyltransferase.

    abstract：:Treatment of the Daudi Burkitt lymphoma-derived cell line with human interferon alpha, which inhibits cell proliferation in this system, induces differentiation of these B-lymphoid cells into cells with a plasmacytoid phenotype. This differentiation, quantified by the appearance of surface antigens characteristic of m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.18.6467

    authors： Exley R,Gordon J,Clemens MJ

    更新日期：1987-09-01 00:00:00

  • Mechanism for discrimination between viral and host mRNA in interferon-treated cells.

    abstract：:In extracts of interferon-treated HeLa cells, RNA covalently linked to double-stranded RNA (dsRNA) is preferentially degraded compared with mRNA not linked in dsRNA. This was established by following the degradation of poly(A)-containing mRNA annealed with poly(U), of poly(C)-containing encephalomyocarditis virus RNA ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.6.2600

    authors： Nilsen TW,Baglioni C

    更新日期：1979-06-01 00:00:00

  • Pathway-based personalized analysis of cancer.

    abstract：:We introduce Pathifier, an algorithm that infers pathway deregulation scores for each tumor sample on the basis of expression data. This score is determined, in a context-specific manner, for every particular dataset and type of cancer that is being investigated. The algorithm transforms gene-level information into pa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1219651110

    authors： Drier Y,Sheffer M,Domany E

    更新日期：2013-04-16 00:00:00