Abstract:
:HIV-1 envelope glycoprotein is the primary target for HIV-1-specific antibodies. The native HIV-1 envelope spike on the virion surface is a trimer, but trimeric gp140 and monomeric gp120 currently are believed to induce comparable immune responses. Indeed, most studies on the immunogenicity of HIV-1 envelope oligomers have revealed only marginal improvement over monomers. We report here that suitably prepared envelope trimers have nearly all the antigenic properties expected for native viral spikes. These stable, rigorously homogenous trimers have antigenic properties markedly different from those of monomeric gp120s derived from the same sequences, and they induce potent neutralizing antibody responses for a cross-clade set of tier 1 and tier 2 viruses with titers substantially higher than those elicited by the corresponding gp120 monomers. These results, which demonstrate that there are relevant immunologic differences between monomers and high-quality envelope trimers, have important implications for HIV-1 vaccine development.
journal_name
Proc Natl Acad Sci U S Aauthors
Kovacs JM,Nkolola JP,Peng H,Cheung A,Perry J,Miller CA,Seaman MS,Barouch DH,Chen Bdoi
10.1073/pnas.1204533109subject
Has Abstractpub_date
2012-07-24 00:00:00pages
12111-6issue
30eissn
0027-8424issn
1091-6490pii
1204533109journal_volume
109pub_type
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