Abstract:
:A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of the isolated gene revealed that human preprocholecystokinin consists of 115 amino acid residues, with 11 amino acids in common with the human gastrin precursor, another member of the gastrin-CCK family, and that the coding region is separated by a single, long intron. CCK appears to be encoded by a single-copy gene in the haploid human genome, as revealed by genomic Southern hybridization analysis, suggesting that the same gene is expressed both in gut and brain.
journal_name
Proc Natl Acad Sci U S Aauthors
Takahashi Y,Kato K,Hayashizaki Y,Wakabayashi T,Ohtsuka E,Matsuki S,Ikehara M,Matsubara Kdoi
10.1073/pnas.82.7.1931subject
Has Abstractpub_date
1985-04-01 00:00:00pages
1931-5issue
7eissn
0027-8424issn
1091-6490journal_volume
82pub_type
杂志文章abstract::The vulval development of Caenorhabditis elegans provides an opportunity to investigate genetic networks that control gene expression during organogenesis. During the fourth larval stage (L4), seven vulval cell types are produced, each of which executes a distinct gene expression program. We analyze how the expression...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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