Abstract:
:The role of cell surface immunoglobulin in helper T-cell-dependent B-cell activation was analyzed using a B-cell lymphoma, CH12, with known antigen specificity and activation properties similar to those of a resting B cell. Two sources of helper T cells were used, both selected such that they interact with H-2-encoded determinants on CH12 in the absence of the specific B-cell antigen, sheep erythrocytes. By this dissociation of the specificity of the T cells from that of the B cells, the requirement for antigen in the induction of CH12 to antibody secretion could be studied. The results show that both helper T-cell-B-cell interactions and surface immunoglobulin-antigen binding are involved in inducing B-cell differentiation, thus establishing a signalling function for the antigen receptor on B lymphocytes. Our data also show that the requirement for surface immunoglobulin-ligand interactions in B-cell activation can, under certain conditions, be circumvented, notably when high (nonphysiologic) multiplicities of T-cell help are used.
journal_name
Proc Natl Acad Sci U S Aauthors
LoCascio NJ,Haughton G,Arnold LW,Corley RBdoi
10.1073/pnas.81.8.2466subject
Has Abstractpub_date
1984-04-01 00:00:00pages
2466-9issue
8eissn
0027-8424issn
1091-6490journal_volume
81pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2015-02-03 00:00:00