Abstract:
:Agrobacterium tumefaciens harboring the tumor-inducing Ti plasmid incites crown gall tumor on dicotyledonous species. Upon infection of these plants, Ti plasmid DNA sequence is stably transferred (T-DNA) by unknown mechanisms to plant cells to be integrated into nuclear DNA. The T-DNA processing and transfer require the expression of vir (virulence) genes on the Ti plasmid, which are known to be induced by certain phenolic compounds released from cells at the wounded inoculation site. The results of the present study demonstrate that wheat and oats contain a substance(s) that induces vir gene expression, yet the inducing substance of wheat differs from the phenolic inducers in that it is hydrophilic and has a molecular weight of several thousand. The novel inducer was not detectable in the exudates of seedlings of these plants but was found in an extract from the transition region between shoot and root of the seedlings and also in extracts from the seeds, bran, and germ. This finding suggests that T-DNA processing and possibly its transfer should take place when Agrobacterium invades suitable tissues of monocotyledonous plants.
journal_name
Proc Natl Acad Sci U S Aauthors
Usami S,Okamoto S,Takebe I,Machida Ydoi
10.1073/pnas.85.11.3748subject
Has Abstractpub_date
1988-06-01 00:00:00pages
3748-52issue
11eissn
0027-8424issn
1091-6490journal_volume
85pub_type
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