Molecular codes for cell type specification in Brn3 retinal ganglion cells.

abstract：:The effects of the thymic polypeptide thymopoietin (Tpo) on the properties of the nicotinic acetylcholine receptor (AcChoR) were investigated by patch clamp techniques on mouse C2 myotubes and by biochemical assays on AcChoR-rich membrane fragments purified from the Torpedo marmorata electric organ. At high concentrat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Revah F,Mulle C,Pinset C,Audhya T,Goldstein G,Changeux JP

更新日期：1987-05-01 00:00:00

abstract：:The gaseous phytohormone ethylene C(2)H(4) mediates numerous aspects of growth and development. Genetic analysis has identified a number of critical elements in ethylene signaling, but how these elements interact biochemically to transduce the signal from the ethylene receptor complex at the endoplasmic reticulum (ER)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ju C,Yoon GM,Shemansky JM,Lin DY,Ying ZI,Chang J,Garrett WM,Kessenbrock M,Groth G,Tucker ML,Cooper B,Kieber JJ,Chang C

更新日期：2012-11-20 00:00:00

abstract：:Protein Hal is a human gamma4 heavy-chain disease protein whose molecular weight is reduced from 55,000 to 25,000 in 6 M guanidine due to the lack of disulfide bonds between heavy chains. Studies of aminoacid sequence indicate that it contains a gap of about 240 residues, starting 10 residues from the N-terminal end a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frangione B,Lee L,Haber E,Bloch KJ

更新日期：1973-04-01 00:00:00

abstract：:Although some important advances in the modeling of sorption and hygrothermal deformations of nanoporous materials such as hydrated cement paste, shale, coal, and some other rocks and soils have already been made, a comprehensive nanoporomechanics theory remains elusive. Here we strive to formulate it based on Gibb's ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen H,Rahimi-Aghdam S,Bažant ZP

更新日期：2020-02-18 00:00:00

abstract：:Hematologic malignancies are frequently associated with cardiac pathologies. Mutations of isocitrate dehydrogenase 1 and 2 (IDH1/2) occur in a subset of acute myeloid leukemia patients, causing metabolic and epigenetic derangements. We have now discovered that altered metabolism in leukemic cells has a profound effect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlstaedt A,Zhang X,Vitrac H,Harmancey R,Vasquez H,Wang JH,Goodell MA,Taegtmeyer H

更新日期：2016-09-13 00:00:00

abstract：:The central questions of bacterial ecology and evolution require a method to consistently demarcate, from the vast and diverse set of bacterial cells within a natural community, the groups playing ecologically distinct roles (ecotypes). Because of a lack of theory-based guidelines, current methods in bacterial systema...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koeppel A,Perry EB,Sikorski J,Krizanc D,Warner A,Ward DM,Rooney AP,Brambilla E,Connor N,Ratcliff RM,Nevo E,Cohan FM

更新日期：2008-02-19 00:00:00

abstract：:The effect of transformation by Rous sarcoma virus on the neural cell adhesion molecule N-CAM was assessed by immunoblotting, immunofluorescence staining, and an in vitro cell-cell aggregation assay using highly specific antibodies to the adhesion molecule. Expression of N-CAM was found to be temperature dependent in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greenberg ME,Brackenbury R,Edelman GM

更新日期：1984-02-01 00:00:00

abstract：:DNase I hypersensitive regions were mapped within the 17.3-kilobase qa (quinic acid) gene cluster of Neurospora crassa. The 5'-flanking regions of the five qa structural genes and the two qa regulatory genes each contain DNase I hypersensitive sites under noninducing conditions and generally exhibit increases in DNase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baum JA,Giles NH

更新日期：1986-09-01 00:00:00

abstract：:The nucleotide and predicted amino acid sequences of two variant cDNAs [rat brain thyroid hormone receptor (rTR alpha) vI and vII], isolated from a rat brain cDNA library by using the Pst I fragment of v-erbA, showed virtual identity with the rat brain thyroid hormone receptor (rTR alpha) [Thompson, C. C., Weinberger,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsuhashi T,Tennyson GE,Nikodem VM

更新日期：1988-08-01 00:00:00

abstract：:Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs. Here we present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishchenko A,Wacker D,Kapoor M,Zhang A,Han GW,Basu S,Patel N,Messerschmidt M,Weierstall U,Liu W,Katritch V,Roth BL,Stevens RC,Cherezov V

更新日期：2017-08-01 00:00:00

abstract：:Class Ib Qa-2 molecules are expressed in tissue culture cells as approximately 40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as approximately 39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tabaczewski P,Shirwan H,Lewis K,Stroynowski I

更新日期：1994-03-01 00:00:00

abstract：:Sex determination in mammals is governed by antagonistic interactions of two genetic pathways, imbalance in which may lead to disorders/differences of sex development (DSD) in human. Among 46,XX individuals with testicular DSD (TDSD) or ovotesticular DSD (OTDSD), testicular tissue is present in the gonad. Although the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eozenou C,Gonen N,Touzon MS,Jorgensen A,Yatsenko SA,Fusee L,Kamel AK,Gellen B,Guercio G,Singh P,Witchel S,Berman AJ,Mainpal R,Totonchi M,Mohseni Meybodi A,Askari M,Merel-Chali T,Bignon-Topalovic J,Migale R,Costanzo

更新日期：2020-06-16 00:00:00

abstract：:In Paramecium tetraurelia, cyclic nucleotides are important physiological second messengers that could regulate dynein mechanochemistry by phosphorylation. A 29-kDa polypeptide that is phosphorylated in a cAMP- and Ca(2+)-sensitive manner in permeabilized cells and isolated axonemes is the only significant phosphoryla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamasaki T,Barkalow K,Richmond J,Satir P

更新日期：1991-09-15 00:00:00

abstract：:A wide range of research has promised new tools for forecasting infectious disease dynamics, but little of that research is currently being applied in practice, because tools do not address key public health needs, do not produce probabilistic forecasts, have not been evaluated on external data, or do not provide suff...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johansson MA,Apfeldorf KM,Dobson S,Devita J,Buczak AL,Baugher B,Moniz LJ,Bagley T,Babin SM,Guven E,Yamana TK,Shaman J,Moschou T,Lothian N,Lane A,Osborne G,Jiang G,Brooks LC,Farrow DC,Hyun S,Tibshirani RJ,Rosenfe

更新日期：2019-11-26 00:00:00

abstract：:Acidification of the extracellular milieu of malignant tumors is reported to increase the invasive behavior of cancer cells. In normal tissues, production of acid is catalyzed by carbonic anhydrases (CAs), some of which are known to be overexpressed in certain cancers. To investigate the functional role of CA activity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parkkila S,Rajaniemi H,Parkkila AK,Kivela J,Waheed A,Pastorekova S,Pastorek J,Sly WS

更新日期：2000-02-29 00:00:00

abstract：:Full-length 66-kDa estrogen receptor alpha (ERalpha) stimulates target gene transcription through two activation functions (AFs), AF-1 in the N-terminal domain and AF-2 in the ligand binding domain. Another physiologically expressed 46-kDa ERalpha isoform lacks the N-terminal A/B domains and is consequently devoid of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Billon-Galés A,Fontaine C,Filipe C,Douin-Echinard V,Fouque MJ,Flouriot G,Gourdy P,Lenfant F,Laurell H,Krust A,Chambon P,Arnal JF

更新日期：2009-02-10 00:00:00

abstract：:The first crystal structure of the neurotransmitter/sodium symporter homolog LeuT revealed an occluded binding pocket containing leucine and 2 Na(+); later structures showed tricyclic antidepressants (TCAs) in an extracellular vestibule approximately 11 A above the bound leucine and 2 Na(+). We recently found this reg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quick M,Winther AM,Shi L,Nissen P,Weinstein H,Javitch JA

更新日期：2009-04-07 00:00:00

abstract：:A G to T mutation has been observed at the third position of codon 249 of the p53 tumor-suppressor gene in over 50% of the hepatocellular carcinoma cases associated with high exposure to aflatoxin B(1) (AFB(1)). Hypotheses have been put forth that AFB(1), in concert with hepatitis B virus (HBV), may play a role in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smela ME,Hamm ML,Henderson PT,Harris CM,Harris TM,Essigmann JM

更新日期：2002-05-14 00:00:00

abstract：:This study examines the basis of resistance and sensitivity of normal and transformed cells to histone deacetylase inhibitor (HDACi)-induced cell death, specifically the role of caspases and thioredoxin (Trx). An important attribute of HDACis is that they induce cancer cell death at concentrations to which normal cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ungerstedt JS,Sowa Y,Xu WS,Shao Y,Dokmanovic M,Perez G,Ngo L,Holmgren A,Jiang X,Marks PA

更新日期：2005-01-18 00:00:00

abstract：:We have evaluated two synthetic epothilone analogues lacking the 12,13-epoxide functionality, 12,13-desoxyepothilone B (dEpoB), and 12,13-desoxyepothilone F (dEpoF). The concentrations required for 50% growth inhibition (IC(50)) for a variety of anticancer agents were measured in CCRF-CEM/VBL1000 cells (2,048-fold res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chou TC,O'Connor OA,Tong WP,Guan Y,Zhang ZG,Stachel SJ,Lee C,Danishefsky SJ

更新日期：2001-07-03 00:00:00

abstract：:The araCBAD regulatory region of Escherichia coli contains two divergently oriented promoters and three sites to which AraC, the regulatory protein of the operon, can bind. This paper presents the results of in vivo dimethyl sulfate "footprinting" experiments to monitor occupancy of the three AraC sites and measuremen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huo L,Martin KJ,Schleif R

更新日期：1988-08-01 00:00:00

abstract：:Recombinant full-length CD4 expressed in Spodoptera frugiperda 9 cells with the baculovirus system was electroinserted in erythrocyte (RBC) membranes. Of the inserted CD4, 70% was "correctly" oriented as shown by fluorescence quenching experiments with fluorescein-labeled CD4. The inserted CD4 displayed the same epito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeira M,Tosi PF,Mouneimne Y,Lazarte J,Sneed L,Volsky DJ,Nicolau C

更新日期：1991-05-15 00:00:00

abstract：:Recent advances in fluorescence microscopy provided tools for the investigation and the analysis of the viral replication steps in the cellular context. In the HIV field, the current visualization systems successfully achieve the fluorescent labeling of the viral envelope and proteins, but not the genome. Here, we dev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Primio C,Quercioli V,Allouch A,Gijsbers R,Christ F,Debyser Z,Arosio D,Cereseto A

更新日期：2013-04-02 00:00:00

abstract：:If they are able to spread in wild populations, CRISPR-based gene-drive elements would provide new ways to address ecological problems by altering the traits of wild organisms, but the potential for uncontrolled spread tremendously complicates ethical development and use. Here, we detail a self-exhausting form of CRIS...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noble C,Min J,Olejarz J,Buchthal J,Chavez A,Smidler AL,DeBenedictis EA,Church GM,Nowak MA,Esvelt KM

更新日期：2019-04-23 00:00:00

abstract：:Infection of the mouse trigeminal ganglia (TG) is the most commonly used model for the study of herpes simplex virus type 1 (HSV-1) latency. Its popularity is caused, at least in part, by the perception that latent infection can be studied in this system in the absence of spontaneous viral reactivation. However, this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldman LT,Ellison AR,Voytek CC,Yang L,Krause P,Margolis TP

更新日期：2002-01-22 00:00:00

abstract：:The proboscis is an important head appendage in insects that has primarily been thought to process gustatory information during food intake. Indeed, in Drosophila and other insects in which they have been identified, most gustatory receptors are expressed in proboscis neurons. Our previous characterization of the expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwon HW,Lu T,Rützler M,Zwiebel LJ

更新日期：2006-09-05 00:00:00

abstract：:We have identified an adenovirus type 5(Ad5) early gene function located in early region 1 which is required for the production of early cytoplasmic mRNAs corresponding to early regions 2, 3, and 4. Mutant dl312 (lacks the segment between 1.5 and 4.5 map units) grows as well as wild-type virus in 293 cells (Ad5-trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones N,Shenk T

更新日期：1979-08-01 00:00:00

abstract：:The template-directed synthesis of a bistable donor-acceptor [2]catenane wherein both translational isomers--one in which a tetrathiafulvalene unit in a mechanically interlocked crown ether occupies the cavity of a cyclobis(paraquat-p-phenylene) ring and the other in which a 1,5-dioxynaphthalene unit in the crown ethe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang C,Olson MA,Fang L,Benítez D,Tkatchouk E,Basu S,Basuray AN,Zhang D,Zhu D,Goddard WA,Stoddart JF

更新日期：2010-08-10 00:00:00

abstract：:The role of tumor necrosis factor alpha (TNF-alpha) in the pathogenesis of autoimmune diabetes mellitus was tested in the nonobese mouse (NOD) model system. The effects of TNF-alpha were assessed on three levels: (i) insulitis development, (ii) development of overt diabetes, (iii) adoptive transfer of diabetes by sple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob CO,Aiso S,Michie SA,McDevitt HO,Acha-Orbea H

更新日期：1990-02-01 00:00:00

abstract：:In the preceding paper we described an experiment that determined the in vivo forward mutation rate in a single replication cycle for spleen necrosis virus. In addition to substitutions, frameshifts, and hypermutations, the mutated proviruses contained two classes of deletions. One class of deletions contained short d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pathak VK,Temin HM

更新日期：1990-08-01 00:00:00