Abstract:
:Visual information is conveyed from the eye to the brain by distinct types of retinal ganglion cells (RGCs). It is largely unknown how RGCs acquire their defining morphological and physiological features and connect to upstream and downstream synaptic partners. The three Brn3/Pou4f transcription factors (TFs) participate in a combinatorial code for RGC type specification, but their exact molecular roles are still unclear. We use deep sequencing to define (i) transcriptomes of Brn3a- and/or Brn3b-positive RGCs, (ii) Brn3a- and/or Brn3b-dependent RGC transcripts, and (iii) transcriptomes of retinorecipient areas of the brain at developmental stages relevant for axon guidance, dendrite formation, and synaptogenesis. We reveal a combinatorial code of TFs, cell surface molecules, and determinants of neuronal morphology that is differentially expressed in specific RGC populations and selectively regulated by Brn3a and/or Brn3b. This comprehensive molecular code provides a basis for understanding neuronal cell type specification in RGCs.
journal_name
Proc Natl Acad Sci U S Aauthors
Sajgo S,Ghinia MG,Brooks M,Kretschmer F,Chuang K,Hiriyanna S,Wu Z,Popescu O,Badea TCdoi
10.1073/pnas.1618551114subject
Has Abstractpub_date
2017-05-16 00:00:00pages
E3974-E3983issue
20eissn
0027-8424issn
1091-6490pii
1618551114journal_volume
114pub_type
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