Initiation of meiotic recombination is independent of interhomologue interactions.

abstract：:Ablation of inhibitory agouti-related protein (AgRP)-expressing neurons in the arcuate nucleus that also synthesize gamma-amino-butyric acid (GABA) and neuropeptide Y in adult mice leads to starvation within 1 week. The removal of inhibition from the AgRP neurons onto neighboring proopiomelanocortin neurons and their ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Q,Howell MP,Cowley MA,Palmiter RD

更新日期：2008-02-19 00:00:00

abstract：:For several decades, the iron-based Fenton-like catalysis has been believed to be mediated by hydroxyl radicals or high-valent iron-oxo species, while only sporadic evidence supported the generation of singlet oxygen (1O2) in the Haber-Weiss cycle. Herein, we report an unprecedented singlet oxygen mediated Fenton-like...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Z,Qian J,Yu A,Pan B

更新日期：2019-04-02 00:00:00

abstract：:Multiplication-stimulating activity (MSA), purified from medium conditioned by the BRL-3A rat liver cell line, previously has been shown to be closely related to the human somatomedins or insulin-like growth factors. A radioimmunoassay was utilized to measure MSA levels in sera from fetal, maternal, and young rats. A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moses AC,Nissley SP,Short PA,Rechler MM,White RM,Knight AB,Higa OZ

更新日期：1980-06-01 00:00:00

abstract：:RecQ helicases are a widely conserved family of ATP-dependent motors with diverse roles in nearly every aspect of bacterial and eukaryotic genome maintenance. However, the physical mechanisms by which RecQ helicases recognize and process specific DNA replication and repair intermediates are largely unknown. Here, we s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pike AC,Gomathinayagam S,Swuec P,Berti M,Zhang Y,Schnecke C,Marino F,von Delft F,Renault L,Costa A,Gileadi O,Vindigni A

更新日期：2015-04-07 00:00:00

abstract：:It has been a long-sought goal of quantum simulation to find answers to outstanding questions in condensed-matter physics. A famous example is finding the ground state and the excitations of the two-dimensional (2D) Hubbard model with strong repulsion below half-filling. This system is a doped antiferromagnet and is o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho TL

更新日期：2020-10-20 00:00:00

abstract：:Calcific aortic valve disease is a chronic inflammatory process, and aortic valve interstitial cells (AVICs) from diseased aortic valves express greater levels of osteogenic factors in response to proinflammatory stimulation. Here, we report that lower cellular levels of IL-37 in AVICs of diseased human aortic valves ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng Q,Song R,Fullerton DA,Ao L,Zhai Y,Li S,Ballak DB,Cleveland JC Jr,Reece TB,McKinsey TA,Xu D,Dinarello CA,Meng X

更新日期：2017-02-14 00:00:00

abstract：:Signal transduction pathways activated by chemoattractants have been extensively studied, but little is known about the events mediating responses to mechanical stimuli. We discovered that acute mechanical perturbation of cells triggered transient activation of all tested components of the chemotactic signal transduct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Artemenko Y,Axiotakis L Jr,Borleis J,Iglesias PA,Devreotes PN

更新日期：2016-11-22 00:00:00

abstract：:RNA-protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA-protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximity biotinylation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han S,Zhao BS,Myers SA,Carr SA,He C,Ting AY

更新日期：2020-09-08 00:00:00

abstract：:The emergence of bacterial multidrug resistance to antibiotics threatens to cause regression to the preantibiotic era. Here we present the crystal structure of the large ribosomal subunit from Staphylococcus aureus, a versatile Gram-positive aggressive pathogen, and its complexes with the known antibiotics linezolid a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyal Z,Matzov D,Krupkin M,Wekselman I,Paukner S,Zimmerman E,Rozenberg H,Bashan A,Yonath A

更新日期：2015-10-27 00:00:00

abstract：:The anticonvulsants phenytoin and carbamazepine interact allosterically with the batrachotoxin binding site of sodium channels. In the present study, we demonstrate an autoradiographic technique to localize the batrachotoxin binding site on sodium channels in rat brain using [3H]batrachotoxinin-A 20-alpha-benzoate (BT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Worley PF,Baraban JM

更新日期：1987-05-01 00:00:00

abstract：:Chlamydia trachomatis is a bacterial pathogen that infects the eyes and urogenital tract. Ocular infection by this organism is the leading cause of preventable blindness worldwide. The infection is also a leading cause of sexually transmitted disease in the United States. As obligate intracellular pathogens, chlamydia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lad SP,Li J,da Silva Correia J,Pan Q,Gadwal S,Ulevitch RJ,Li E

更新日期：2007-02-20 00:00:00

abstract：:NK cells are promising effectors for tumor adoptive immunotherapy, particularly when considering the targeting of MHC class I low or negative tumors. Yet, NK cells cannot respond to many tumors, which is particularly the case for nonhematopoietic tumors such as carcinomas or melanoma even when these cells lose MHC cla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kruschinski A,Moosmann A,Poschke I,Norell H,Chmielewski M,Seliger B,Kiessling R,Blankenstein T,Abken H,Charo J

更新日期：2008-11-11 00:00:00

abstract：:The Drosophila melanogaster urate oxidase (UO)-encoding gene is expressed in the third-instar larva and adult. In contrast, the Drosophila pseudoobscura UO gene is only expressed in the adult, whereas the Drosophila virilis UO gene is expressed only in the third-instar larva. UO activity in these three Drosophila spec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallrath LL,Friedman TB

更新日期：1991-07-01 00:00:00

abstract：:A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed. Mutant bacteria grow normally, at 41 degrees as well as at 30 degrees . Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirota Y,Gefter M,Mindich L

更新日期：1972-11-01 00:00:00

abstract：:The transcription factor cAMP response element (CRE)-binding protein (CREB) has been shown to regulate neural plasticity. Drugs of abuse activate CREB in the nucleus accumbens, an important part of the brain's reward pathways, and local manipulations of CREB activity have been shown to affect cocaine reward, suggestin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrot M,Olivier JD,Perrotti LI,DiLeone RJ,Berton O,Eisch AJ,Impey S,Storm DR,Neve RL,Yin JC,Zachariou V,Nestler EJ

更新日期：2002-08-20 00:00:00

abstract：:Translocation of proteins across the mitochondrial inner membrane is an essential process requiring an import motor having mitochondrial Hsp70 (mtHsp70) at its core. The J protein partner of mtHsp70, Pam18, is an integral part of this motor, serving to stimulate the ATPase activity of mtHsp70. Pam16, an essential prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'Silva PR,Schilke B,Walter W,Craig EA

更新日期：2005-08-30 00:00:00

abstract：:Nitrogenase is an ATP-requiring enzyme capable of carrying out multielectron reductions of inert molecules. A purified remodeled nitrogenase containing two amino acid substitutions near the site of its FeMo cofactor was recently described as having the capacity to reduce carbon dioxide (CO2) to methane (CH4). Here, we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fixen KR,Zheng Y,Harris DF,Shaw S,Yang ZY,Dean DR,Seefeldt LC,Harwood CS

更新日期：2016-09-06 00:00:00

abstract：:We have analyzed the cell cycle regulation of human cyclin-dependent kinase 2 (CDK2), a protein closely related to the cell cycle-regulatory protein kinase CDC2. We find that CDK2 activity, like that of CDC2, oscillates during the cell cycle in cultured mammalian fibroblasts. Unlike CDC2 activity (which peaks during m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenblatt J,Gu Y,Morgan DO

更新日期：1992-04-01 00:00:00

abstract：:Peroxiredoxins (Prx), a family of peroxidases that reduce intracellular peroxides with the thioredoxin system as the electron donor, are highly expressed in various cellular compartments. Among the antioxidant Prx enzymes, Prx2 is the most abundant in mammalian neurons, making it a prime candidate to defend against ox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fang J,Nakamura T,Cho DH,Gu Z,Lipton SA

更新日期：2007-11-20 00:00:00

abstract：:The incidence of pediatric adrenal cortical carcinoma (ACC) in southern Brazil is 10-15 times higher than that of pediatric ACC worldwide. Because childhood ACC is associated with Li-Fraumeni syndrome, we examined the cancer history and p53 status of 36 Brazilian patients and their families. Remarkably, 35 of 36 patie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ribeiro RC,Sandrini F,Figueiredo B,Zambetti GP,Michalkiewicz E,Lafferty AR,DeLacerda L,Rabin M,Cadwell C,Sampaio G,Cat I,Stratakis CA,Sandrini R

更新日期：2001-07-31 00:00:00

abstract：:Interleukin 16 (IL-16) is a chemotactic cytokine that binds to the CD4 receptor and affects the activation of T cells and replication of HIV. It is expressed as a large 67-kDa precursor protein (pro-IL-16) in lymphocytes, macrophages, and mast cells, as well as in airway epithelial cells from asthmatics after challeng...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bannert N,Avots A,Baier M,Serfling E,Kurth R

更新日期：1999-02-16 00:00:00

abstract：:We have developed a method for conjugating low density lipoproteins (LDL) with colloidal gold. Conjugation, complete after 1 min, occurs by electrostatic adsorption of the LDL to the negatively charged gold particle. Each conjugate consists of approximately eight biologically active LDL molecules clustered around a ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Handley DA,Arbeeny CM,Witte LD,Chien S

更新日期：1981-01-01 00:00:00

abstract：:We used a high-titer recombinant adeno-associated virus (rAAV) vector to express WT or mutant human alpha-synuclein in the substantia nigra of adult marmosets. The alpha-synuclein protein was expressed in 90-95% of all nigral dopamine neurons and distributed by anterograde transport throughout their axonal and dendrit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirik D,Annett LE,Burger C,Muzyczka N,Mandel RJ,Björklund A

更新日期：2003-03-04 00:00:00

abstract：:Oligodeoxyribonucleotides covalently linked to an intercalating agent via a polymethylene linker were synthesized. Oligothymidylates attached to an acridine dye (Acr) through the 3'-phosphate group [(Tp)n(CH2) mAcr ] specifically interact with the complementary sequence. The interaction is strongly stabilized by the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asseline U,Delarue M,Lancelot G,Toulmé F,Thuong NT,Montenay-Garestier T,Hélène C

更新日期：1984-06-01 00:00:00

abstract：:An extracellular matrix protein, cytotactin, with widespread tissue distribution has been identified, isolated, and partially characterized. Cytotactin mediates glia-neuron adhesion in vitro, but unlike Ng-CAM, the neuron-glia cell-adhesion molecule, it is absent from neurons. Cytotactin was isolated from 14-day embry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grumet M,Hoffman S,Crossin KL,Edelman GM

更新日期：1985-12-01 00:00:00

abstract：:The localization of endothelin 1 mRNA and endothelin-like immunoreactivity was investigated in samples of neurologically normal nervous system tissue from 10 adults by using in situ hybridization and immunocytochemistry. Tissue sections of spinal cord and dorsal root ganglia were hybridized with an 35S-radiolabeled en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giaid A,Gibson SJ,Ibrahim BN,Legon S,Bloom SR,Yanagisawa M,Masaki T,Varndell IM,Polak JM

更新日期：1989-10-01 00:00:00

abstract：:Regulated intramembrane proteolysis by gamma-secretase cleaves proteins in their transmembrane domain and is involved in important signaling pathways. At least four different gamma-secretase complexes have been identified, but little is known about their biological role and specificity. Previous work has demonstrated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dejaegere T,Serneels L,Schäfer MK,Van Biervliet J,Horré K,Depboylu C,Alvarez-Fischer D,Herreman A,Willem M,Haass C,Höglinger GU,D'Hooge R,De Strooper B

更新日期：2008-07-15 00:00:00

abstract：:The techniques of in vivo magnetic resonance (MR) imaging and spectroscopy have been established over the past two decades. Recent applications of these methods to study human brain function have become a rapidly growing area of research. The development of methods using standard MR contrast agents within the cerebral...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shulman RG,Blamire AM,Rothman DL,McCarthy G

更新日期：1993-04-15 00:00:00

abstract：:V79 Chinese hamster fibroblasts are widely used for mutagenicity testing but have the serious limitation that they do not express cytochromes P-450, which are needed for the activation of many promutagens to mutagenic metabolites. A full-length cDNA clone encoding the monooxygenase cytochrome P-450IIB1 under control o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doehmer J,Dogra S,Friedberg T,Monier S,Adesnik M,Glatt H,Oesch F

更新日期：1988-08-01 00:00:00

abstract：:Preclinical animal models have largely ignored the immune-suppressive mechanisms that are important in human cancers. The identification and use of such models should allow better predictions of successful human responses to immunotherapy. As a model for changes induced in nonmalignant cells by cancer, we examined T-c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorgun G,Ramsay AG,Holderried TA,Zahrieh D,Le Dieu R,Liu F,Quackenbush J,Croce CM,Gribben JG

更新日期：2009-04-14 00:00:00