Engineering antigen-specific primary human NK cells against HER-2 positive carcinomas.

abstract：:Plasmids have been constructed to generate substrates for the study of proteinases 2A and 3C of poliovirus. They contain the P1 (capsomer precursor) region of the poliovirus genome or P1 and part of P2 (a nonstructural precursor), which can be transcribed and translated in vitro. A transcript containing the entire 5' ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicklin MJ,Kräusslich HG,Toyoda H,Dunn JJ,Wimmer E

更新日期：1987-06-01 00:00:00

abstract：:Evidence suggests that cholinergic input to the hippocampus plays an important role in learning and memory and that degeneration of cholinergic terminals in the hippocampus may contribute to the memory loss associated with Alzheimer's disease. One of the more prominent effects of cholinergic agonists on hippocampal ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marino MJ,Rouse ST,Levey AI,Potter LT,Conn PJ

更新日期：1998-09-15 00:00:00

abstract：:To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Peca J,Matsuzaki M,Matsuzaki K,Noguchi J,Qiu L,Wang D,Zhang F,Boyden E,Deisseroth K,Kasai H,Hall WC,Feng G,Augustine GJ

更新日期：2007-05-08 00:00:00

abstract：:Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brabec RK,Peters BP,Bernstein IA,Gray RH,Goldstein IJ

更新日期：1980-01-01 00:00:00

abstract：:In this paper I review the ways in which the glassy state is obtained both in nature and in materials science and highlight a "new twist"--the recent recognition of polymorphism within the glassy state. The formation of glass by continuous cooling (viscous slowdown) is then examined, the strong/fragile liquids classif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angell CA

更新日期：1995-07-18 00:00:00

abstract：:Resting- or baseline-state low-frequency (0.01-0.2 Hz) brain activity is observed in fMRI, EEG, and local field potential recordings. These fluctuations were found to be correlated across brain regions and are thought to reflect neuronal activity fluctuations between functionally connected areas of the brain. However,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krishnan GP,González OC,Bazhenov M

更新日期：2018-06-26 00:00:00

abstract：:The availability of gene-targeted mice deficient in the urokinase-type plasminogen activator (uPA), urokinase receptor (uPAR), tissue-type plasminogen activator (tPA), and plasminogen permits a critical, genetic-based analysis of the physiological and pathological roles of the two mammalian plasminogen activators. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bugge TH,Flick MJ,Danton MJ,Daugherty CC,Romer J,Dano K,Carmeliet P,Collen D,Degen JL

更新日期：1996-06-11 00:00:00

abstract：:We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bukrinsky MI,Sharova N,McDonald TL,Pushkarskaya T,Tarpley WG,Stevenson M

更新日期：1993-07-01 00:00:00

abstract：:Ducks and wild waterfowl perpetuate all strains of influenza viruses in nature. In their natural host, influenza viruses typically cause asymptomatic infection and little pathology. Ducks are often resistant to influenza viruses capable of killing chickens. Here, we show that the influenza virus sensor, RIG-I, is pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barber MR,Aldridge JR Jr,Webster RG,Magor KE

更新日期：2010-03-30 00:00:00

abstract：:The mechanisms that are essential for the maintenance of nutrient status in breast milk are unclear. Our data demonstrate that the intestine via hypoxia-inducible factor (HIF)-2α is an essential regulatory mechanism for maintaining the quality of breast milk. During lactation, intestinal HIF-2α is highly increased, le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramakrishnan SK,Anderson ER,Martin A,Centofanti B,Shah YM

更新日期：2015-07-14 00:00:00

abstract：:Ionizing radiation has been used to selectively separate the circadian oscillator function of the eye of Aplysia from some of its other functions--synchronous compound action potential (CAP) generation, the light response, synaptic transmission between photoreceptors and output neurons, and the bursting pacemaker mech...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woolum JC,Strumwasser F

更新日期：1980-09-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease in the tropics and subtropics with high morbidity and mortality. The facultative intracellular bacterium induces host cell fusion through its type VI secretion system 5 (T6SS5) as an important part of its pathogenesis in mammalian h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ku JWK,Chen Y,Lim BJW,Gasser S,Crasta KC,Gan YH

更新日期：2020-07-07 00:00:00

abstract：:Thermal macrophysiology is an established research field that has led to well-described patterns in the global structuring of climate adaptation and risk. However, since it was developed primarily in animals, we lack information on how general these patterns are across organisms. This is alarming if we are to understa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lancaster LT,Humphreys AM

更新日期：2020-06-16 00:00:00

abstract：:The functional role of residue Tyr-19 of Chromatium vinosum HiPIP has been evaluated by site-directed mutagenesis experiments. The stability of the [Fe4S4] cluster prosthetic center is sensitive to side-chain replacements. Polar residues result in significant instability, while nonpolar residues (especially with aroma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal A,Li D,Cowan JA

更新日期：1995-10-10 00:00:00

abstract：:Nanophthalmos is a rare disorder of eye development characterized by extreme hyperopia (farsightedness), with refractive error in the range of +8.00 to +25.00 diopters. Because the cornea and lens are normal in size and shape, hyperopia occurs because insufficient growth along the visual axis places these lensing comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sundin OH,Leppert GS,Silva ED,Yang JM,Dharmaraj S,Maumenee IH,Santos LC,Parsa CF,Traboulsi EI,Broman KW,Dibernardo C,Sunness JS,Toy J,Weinberg EM

更新日期：2005-07-05 00:00:00

abstract：:Mannan-binding protein (MBP), a Ca2+-dependent mammalian lectin specific for mannose and N-acetylglucosamine, is an important serum component associated with innate immunity. MBP activates complement and functions as a direct opsonin on binding to mannooligosaccharide-bearing pathogens. We have found that MBP recogniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Y,Uemura K,Oka S,Kozutsumi Y,Kawasaki N,Kawasaki T

更新日期：1999-01-19 00:00:00

abstract：:The human fungal pathogen Candida albicans shows a significant lag in growth when diluted into fresh minimal medium. This lag is abolished by the addition of conditioned medium from a high-density culture. The active component of conditioned medium is tyrosol, which is released into the medium continuously during grow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen H,Fujita M,Feng Q,Clardy J,Fink GR

更新日期：2004-04-06 00:00:00

abstract：:The 5'-phosphoinositol phosphatase SHIP negatively regulates signaling pathways triggered by antigen, cytokine and Fc receptors in both lymphocytes and myeloid cells. Mice with germ-line (null) deletion of SHIP develop a myeloproliferative-like syndrome that causes early lethality. Lymphocyte anomalies have been obser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tarasenko T,Kole HK,Chi AW,Mentink-Kane MM,Wynn TA,Bolland S

更新日期：2007-07-03 00:00:00

abstract：:The lymphoma-inducing potential of Ig heavy-chain enhancer- and promoter-regulated Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) was evaluated in three transgenic FVB mouse lineages. EBNA1 was expressed at a higher level in transgenic B220(+) splenocytes than in EBV-infected lymphoblastoid cell lines. EBNA1 was a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang MS,Lu H,Yasui T,Sharpe A,Warren H,Cahir-McFarland E,Bronson R,Hung SC,Kieff E

更新日期：2005-01-18 00:00:00

abstract：:Progress in ecosystem service science has been rapid, and there is now a healthy appetite among key public and private sector decision makers for this science. However, changing policy and management is a long-term project, one that raises a number of specific practical challenges. One impediment to broad adoption of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polasky S,Tallis H,Reyers B

更新日期：2015-06-16 00:00:00

abstract：:mTOR is an evolutionarily conserved kinase that plays a critical role in sensing and responding to environmental determinants. Recent studies have shown that fine-tuning of the activity of mTOR complexes contributes to organogenesis and tumorigenesis. Although rapamycin, an allosteric mTOR inhibitor, is an effective i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoshii T,Kasada A,Hatakeyama T,Ohtani M,Tadokoro Y,Naka K,Ikenoue T,Ikawa T,Kawamoto H,Fehling HJ,Araki K,Yamamura K,Matsuda S,Hirao A

更新日期：2014-03-11 00:00:00

abstract：:The ASCAPE agent-based modeling environment greatly eases the task of designing and investigating agent-based models. However, effective design can still require a relatively deep knowledge of programming and agent-based modeling. This issue has almost certainly slowed the adoption of agent-based modeling approaches t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inchiosa ME,Parker MT

更新日期：2002-05-14 00:00:00

abstract：:We have employed a combination of temperature-dependent UV absorption spectroscopy, circular dichroism, and batch calorimetry to characterize the binding of actinomycin D to a series of oligomeric DNA duplexes. We find the duplex [d(CGTCGACG)]2 to be unique in its ability to bind actinomycin D strongly despite the abs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Snyder JG,Hartman NG,D'Estantoit BL,Kennard O,Remeta DP,Breslauer KJ

更新日期：1989-06-01 00:00:00

abstract：:The plant trans-Golgi network (TGN) is a central trafficking hub where secretory, vacuolar, recycling, and endocytic pathways merge. Among currently known molecular players involved in TGN transport, three different adaptor protein (AP) complexes promote vesicle generation at the TGN with different cargo specificity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heinze L,Freimuth N,Rößling AK,Hahnke R,Riebschläger S,Fröhlich A,Sampathkumar A,McFarlane HE,Sauer M

更新日期：2020-10-13 00:00:00

abstract：:We report a molecular analysis of the chicken Ig loci in single bursal follicles from 3- to 7-week-old chickens. Each follicle contained between 10(5) and 3 X 10(5) cells. The Ig gene rearrangement patterns obtained were compared to the pattern observed with the corresponding total bursal DNA. The results obtained for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weill JC,Reynaud CA,Lassila O,Pink JR

更新日期：1986-05-01 00:00:00

abstract：:Antibodies against an inositol phospholipid-specific phospholipase C purified from bovine brain were used to screen rat brain lambda gt11 expression cDNA libraries. Complete sequences of three cDNA inserts yielded a cumulative sequence of 5106 base pairs. The deduced protein had 1289 amino acids with a calculated mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suh PG,Ryu SH,Moon KH,Suh HW,Rhee SG

更新日期：1988-08-01 00:00:00

abstract：:Tissue kallikrein (TK) is a serine protease synthetized in renal tubular cells located upstream from the collecting duct where renal potassium balance is regulated. Because secretion of TK is promoted by K+ intake, we hypothesized that this enzyme might regulate plasma K+ concentration ([K+]). We showed in wild-type m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El Moghrabi S,Houillier P,Picard N,Sohet F,Wootla B,Bloch-Faure M,Leviel F,Cheval L,Frische S,Meneton P,Eladari D,Chambrey R

更新日期：2010-07-27 00:00:00

abstract：:The study of development has relied primarily on the isolation of mutations in genes with specific functions in development and on the comparison of their expression patterns in normal and mutant phenotypes. Comparative evolutionary analyses can complement these approaches. Phylogenetic analyses of Sonic hedgehog (Shh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zardoya R,Abouheif E,Meyer A

更新日期：1996-11-12 00:00:00

abstract：:As new disease threats arise and existing pathogens grow resistant to conventional interventions, attention increasingly focuses on the development of vaccines to induce protective immune responses. Given their admirable safety records, protein subunit vaccines are attractive for widespread immunization, but their dis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen DJ,Osterrieder N,Metzger SM,Buckles E,Doody AM,DeLisa MP,Putnam D

更新日期：2010-02-16 00:00:00

abstract：:The neurotrophin (NT) brain-derived neurotrophic factor (BDNF) plays an essential role in the formation of long-term potentiation (LTP). Here, we address whether this modulation by BDNF requires its continuous presence, or whether a local increase in BDNF is necessary during a specific time period of LTP initiation. U...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gärtner A,Staiger V

更新日期：2002-04-30 00:00:00