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Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium.

Abstract：

:Nitrogenase is an ATP-requiring enzyme capable of carrying out multielectron reductions of inert molecules. A purified remodeled nitrogenase containing two amino acid substitutions near the site of its FeMo cofactor was recently described as having the capacity to reduce carbon dioxide (CO2) to methane (CH4). Here, we developed the anoxygenic phototroph, Rhodopseudomonas palustris, as a biocatalyst capable of light-driven CO2 reduction to CH4 in vivo using this remodeled nitrogenase. Conversion of CO2 to CH4 by R. palustris required constitutive expression of nitrogenase, which was achieved by using a variant of the transcription factor NifA that is able to activate expression of nitrogenase under all growth conditions. Also, light was required for generation of ATP by cyclic photophosphorylation. CH4 production by R. palustris could be controlled by manipulating the distribution of electrons and energy available to nitrogenase. This work shows the feasibility of using microbes to generate hydrocarbons from CO2 in one enzymatic step using light energy.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Fixen KR,Zheng Y,Harris DF,Shaw S,Yang ZY,Dean DR,Seefeldt LC,Harwood CS

doi

10.1073/pnas.1611043113

subject

Has Abstract

pub_date

2016-09-06 00:00:00

pages

10163-7

issue

36

eissn

0027-8424

issn

1091-6490

pii

1611043113

journal_volume

113

pub_type

杂志文章

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