Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.

Abstract:

:Hsubc9, a human gene encoding a ubiquitin-conjugating enzyme, has been cloned. The 18-kDa HsUbc9 protein is homologous to the ubiquitin-conjugating enzymes Hus5 of Schizosaccharomyces pombe and Ubc9 of Saccharomyces cerevisiae. The Hsubc9 gene complements a ubc9 mutation of S. cerevisiae. It has been mapped to chromosome 16p13.3 and is expressed in many human tissues, with the highest levels in testis and thymus. According to the Ga14 two-hybrid system analysis, HsUbc9 protein interacts with human recombination protein Rad51. A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein. In mouse spermatocytes, MmUbc9 protein, like Rad51 protein, localizes in synaptonemal complexes, which suggests that Ubc9 protein plays a regulatory role in meiosis.

authors

Kovalenko OV,Plug AW,Haaf T,Gonda DK,Ashley T,Ward DC,Radding CM,Golub EI

doi

10.1073/pnas.93.7.2958

subject

Has Abstract

pub_date

1996-04-02 00:00:00

pages

2958-63

issue

7

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章
  • Speech-like cerebral activity in profoundly deaf people processing signed languages: implications for the neural basis of human language.

    abstract::For more than a century we have understood that our brain's left hemisphere is the primary site for processing language, yet why this is so has remained more elusive. Using positron emission tomography, we report cerebral blood flow activity in profoundly deaf signers processing specific aspects of sign language in ke...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.25.13961

    authors: Petitto LA,Zatorre RJ,Gauna K,Nikelski EJ,Dostie D,Evans AC

    更新日期:2000-12-05 00:00:00

  • Sequential repression and activation of the CCAAT enhancer-binding protein-alpha (C/EBPalpha ) gene during adipogenesis.

    abstract::CCAAT enhancer-binding protein-alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of adipocyte genes during adipogenesis. Nuclear factor C/EBP undifferentiated protein (CUP), an isoform of activator protein-2alpha (AP-2alpha), binds to repressive elements in the C/EBPalpha gene promoter, silencing...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.220426097

    authors: Jiang MS,Lane MD

    更新日期:2000-11-07 00:00:00

  • Body map proto-organization in newborn macaques.

    abstract::Topographic sensory maps are a prominent feature of the adult primate brain. Here, we asked whether topographic representations of the body are present at birth. Using functional MRI (fMRI), we find that the newborn somatomotor system, spanning frontoparietal cortex and subcortex, comprises multiple topographic repres...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1912636116

    authors: Arcaro MJ,Schade PF,Livingstone MS

    更新日期:2019-12-03 00:00:00

  • The functional analysis of insulator interactions in the Drosophila embryo.

    abstract::Chromatin boundaries or insulators modulate enhancer-promoter interactions in complex genetic loci. However, the mechanism underlying insulator activity is not known. Previous studies showed that the activity of the Drosophila suHw insulator is abolished by the tandem arrangement (pairing) of the insulator elements, s...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0830190100

    authors: Majumder P,Cai HN

    更新日期:2003-04-29 00:00:00

  • Periodicity of extinctions in the geologic past.

    abstract::The temporal distribution of the major extinctions over the past 250 million years has been investigated statistically using various forms of time series analysis. The analyzed record is based on variation in extinction intensity for fossil families of marine vertebrates, invertebrates, and protozoans and contains 12 ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.3.801

    authors: Raup DM,Sepkoski JJ Jr

    更新日期:1984-02-01 00:00:00

  • Binary complex crystal structure of DNA polymerase β reveals multiple conformations of the templating 8-oxoguanine lesion.

    abstract::Oxidation of genomic DNA forms the guanine lesion 7,8-dihydro-8-oxoguanine (8-oxoG). When in the template base position during DNA synthesis the 8-oxoG lesion has dual coding potential by virtue of its anti- and syn-conformations, base pairing with cytosine and adenine, respectively. This impacts mutagenesis, because ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1112235108

    authors: Batra VK,Shock DD,Beard WA,McKenna CE,Wilson SH

    更新日期:2012-01-03 00:00:00

  • A mutation in the alpha 3 chain of type IX collagen causes autosomal dominant multiple epiphyseal dysplasia with mild myopathy.

    abstract::Multiple epiphyseal dysplasia (MED) is a degenerative cartilage condition shown in some cases to be caused by mutations in genes encoding cartilage oligomeric matrix protein or type IX collagen. We studied a family with autosomal dominant MED affecting predominantly the knee joints and a mild proximal myopathy. Geneti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.3.1212

    authors: Bönnemann CG,Cox GF,Shapiro F,Wu JJ,Feener CA,Thompson TG,Anthony DC,Eyre DR,Darras BT,Kunkel LM

    更新日期:2000-02-01 00:00:00

  • Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells.

    abstract::In FRTL5 rat thyroid cells, norepinephrine, by interacting with alpha 1-adrenergic receptors, stimulates inositol phosphate formation, through activation of phospholipase C, and arachidonic acid release. Recent studies have shown that GTP-binding proteins couple several types of receptors to phospholipase C activation...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.19.7201

    authors: Burch RM,Luini A,Axelrod J

    更新日期:1986-10-01 00:00:00

  • In vivo phosphorylation of the Na,K-ATPase alpha subunit in sciatic nerves of control and diabetic rats: effects of protein kinase modulators.

    abstract::The phosphorylation state of the Na,K-ATPase alpha subunit has been examined in 32P-labeled sciatic nerves of control and streptozotocin-treated diabetic rats. Intact nerves were challenged with protein kinase (PK) modulators and alpha-subunit 32P labeling was analyzed after immunoprecipitation. In control nerves, the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.13.6211

    authors: Borghini I,Geering K,Gjinovci A,Wollheim CB,Pralong WF

    更新日期:1994-06-21 00:00:00

  • DNA length, bending, and twisting constraints on IS50 transposition.

    abstract::Transposition is a multistep process in which a transposable element DNA sequence moves from its original genetic location to a new site. Early steps in this process include the formation of a transposition complex in which the end sequences of the transposable element are brought together in a structurally precise fa...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.23.10834

    authors: Goryshin IYu,Kil YV,Reznikoff WS

    更新日期:1994-11-08 00:00:00

  • Attenuated Mengo virus as a vector for immunogenic human immunodeficiency virus type 1 glycoprotein 120.

    abstract::Introduction of a sequence encoding 147 amino acids from human immunodeficiency virus type I (HIV-1) strain MN glycoprotein gp120 into the RNA genome of the stably attenuated Mengo virus strain vM16 yielded an infectious recombinant virus, vMLN450, which expressed the heterologous HIV-1 sequence along with the normal ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.21.9775

    authors: Altmeyer R,Escriou N,Girard M,Palmenberg A,van der Werf S

    更新日期:1994-10-11 00:00:00

  • Mutagenesis during in vitro DNA synthesis.

    abstract::The error frequency of in vitro DNA synthesis using a natural DNA template has been measured with a biological assay for nucleotide substitutions. phiX174 DNA containing an amber mutation was copied in vitro by Escherichia coli DNA polymerase I, and the reversion frequency of the progeny DNA was determined by transfec...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.4.1924

    authors: Weymouth LA,Loeb LA

    更新日期:1978-04-01 00:00:00

  • Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals.

    abstract::High-quality protein crystals meant for structural analysis by X-ray diffraction have been grown by various methods. The observation of dynamical diffraction in protein crystals is an interesting topic because dynamical diffraction generally occurs in perfect crystals such as Si crystals. However, to our knowledge, th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1720098115

    authors: Suzuki R,Koizumi H,Hirano K,Kumasaka T,Kojima K,Tachibana M

    更新日期:2018-04-03 00:00:00

  • Different missense mutations at the tissue-nonspecific alkaline phosphatase gene locus in autosomal recessively inherited forms of mild and severe hypophosphatasia.

    abstract::Hypophosphatasia is a heritable form of rickets/osteomalacia with extremely variable clinical expression. Severe forms are inherited in an autosomal recessive fashion; the mode of transmission of mild forms is uncertain. The biochemical hallmark of hypophosphatasia is deficient activity of the tissue-nonspecific isozy...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.20.9924

    authors: Henthorn PS,Raducha M,Fedde KN,Lafferty MA,Whyte MP

    更新日期:1992-10-15 00:00:00

  • Regulatory interactions of calmodulin-binding proteins: phosphorylation of calcineurin by autophosphorylated Ca2+/calmodulin-dependent protein kinase II.

    abstract::The Ca2+/calmodulin (CaM)-dependent protein phosphatase calcineurin is rapidly phosphorylated (0.8 mol of 32PO4 per mol of 60-kDa subunit of calcineurin) by brain Ca2+/CaM-dependent protein kinase II (CaM-kinase II). This reaction requires the autophosphorylated, Ca2+-independent form of CaM-kinase II since Ca2+/CaM b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.18.7001

    authors: Hashimoto Y,King MM,Soderling TR

    更新日期:1988-09-01 00:00:00

  • Proteomic and genomic signatures of repeat instability in cancer and adjacent normal tissues.

    abstract::Repetitive sequences are hotspots of evolution at multiple levels. However, due to difficulties involved in their assembly and analysis, the role of repeats in tumor evolution is poorly understood. We developed a rigorous motif-based methodology to quantify variations in the repeat content, beyond microsatellites, in ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1908790116

    authors: Persi E,Prandi D,Wolf YI,Pozniak Y,Barnabas GD,Levanon K,Barshack I,Barbieri C,Gasperini P,Beltran H,Faltas BM,Rubin MA,Geiger T,Koonin EV,Demichelis F,Horn D

    更新日期:2019-08-20 00:00:00

  • Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate binding pocket.

    abstract::The substrate specificity of thermophilic xylose isomerase from Clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the Arthrobacter enzyme and site-directed mutagenesis of the thermophile xylA gene. The orientation of glucose as a substrate in the active site of the th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.9.4015

    authors: Meng M,Lee C,Bagdasarian M,Zeikus JG

    更新日期:1991-05-01 00:00:00

  • recF-dependent induction of recA synthesis by coumermycin, a specific inhibitor of the B subunit of DNA gyrase.

    abstract::Genetic and biochemical studies on enzymes known to be involved in regulating DNA supercoiling yield a complex spectrum of effects on the Escherichia coli SOS system. Previous studies indicated that only inhibition of DNA gyrase by antibiotics that act on the DNA gyrase A subunit results in turning on the E. coli SOS ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.9.2510

    authors: Smith CL

    更新日期:1983-05-01 00:00:00

  • Some features of the spread of epidemics and information on a random graph.

    abstract::Random graphs are useful models of social and technological networks. To date, most of the research in this area has concerned geometric properties of the graphs. Here we focus on processes taking place on the network. In particular we are interested in how their behavior on networks differs from that in homogeneously...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0914402107

    authors: Durrett R

    更新日期:2010-03-09 00:00:00

  • Universal poroelastic mechanism for hydraulic signals in biomimetic and natural branches.

    abstract::Plants constantly undergo external mechanical loads such as wind or touch and respond to these stimuli by acclimating their growth processes. A fascinating feature of this mechanical-induced growth response is that it can occur rapidly and at long distance from the initial site of stimulation, suggesting the existence...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1707675114

    authors: Louf JF,Guéna G,Badel E,Forterre Y

    更新日期:2017-10-17 00:00:00

  • Identification of a point mutation in the catalytic domain of the protooncogene c-kit in peripheral blood mononuclear cells of patients who have mastocytosis with an associated hematologic disorder.

    abstract::Both stem cells and mast cells express c-kit and proliferate after exposure to c-kit ligand. Mutations in c-kit may enhance or interfere with the ability of c-kit receptor to initiate the intracellular pathways resulting in cell proliferation. These observations suggested to us that mastocytosis might in some patients...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.23.10560

    authors: Nagata H,Worobec AS,Oh CK,Chowdhury BA,Tannenbaum S,Suzuki Y,Metcalfe DD

    更新日期:1995-11-07 00:00:00

  • A role for LYNX2 in anxiety-related behavior.

    abstract::Anxiety disorders are the most prevalent mental disorders in developed societies. Although roles for the prefrontal cortex, amygdala, hippocampus and mediodorsal thalamus in anxiety disorders are well documented, molecular mechanisms contributing to the functions of these structures are poorly understood. Here we repo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0813109106

    authors: Tekinay AB,Nong Y,Miwa JM,Lieberam I,Ibanez-Tallon I,Greengard P,Heintz N

    更新日期:2009-03-17 00:00:00

  • Evolutionary and structural influences on light chain constant (CL) region of human and mouse immunoglobulins.

    abstract::A comparison of five constant region sequences of human and mouse k and lambda immunoglobulin chains has been undertaken in order to reveal sequence homologies and evolutionary relationships. Simultaneously, a comparison with the three-dimensional structure of one mouse k-chain (McPC 603) has suggested structural reas...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.7.2785

    authors: Kabat EA,Padlan EA,Davies DR

    更新日期:1975-07-01 00:00:00

  • Hydrogen peroxide production, chemiluminescence, and the respiratory burst of fertilization: interrelated events in early sea urchin development.

    abstract::After fertilization of the sea urchin, Strongyl-ocentrotus purpuratus, a crosslinked fertilization membrane is formed; the crosslinks (dityrosine residues) are synthesized in a reaction catalyzed by an ovoperoxidase that is released from the cortical granules during fertilization. The substrate for ovoperoxidase activ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.7.3183

    authors: Foerder CA,Klebanoff SJ,Shapiro BM

    更新日期:1978-07-01 00:00:00

  • The three-dimensional structure of an H-2Ld-peptide complex explains the unique interaction of Ld with beta-2 microglobulin and peptide.

    abstract::Solution at 2.5-A resolution of the three-dimensional structure of H-2Ld with a single nine-residue peptide provides a structural basis for understanding its unique interaction with beta-2 microglobulin (beta2m) and peptide. Consistent with the biological data that show an unusually weak association of Ld with beta2m,...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.13.6880

    authors: Balendiran GK,Solheim JC,Young AC,Hansen TH,Nathenson SG,Sacchettini JC

    更新日期:1997-06-24 00:00:00

  • Ly49Q, a member of the Ly49 family that is selectively expressed on myeloid lineage cells and involved in regulation of cytoskeletal architecture.

    abstract::Here, we identified and characterized a Ly49 family member, designated as Ly49Q. The Ly49q gene encodes a 273-aa protein with an immunoreceptor tyrosine-based inhibitory motif (ITIM) at the N terminus of its cytoplasmic domain. We show that the ITIM of Ly49Q can recruit SHP-2 and SHP-1 in a tyrosine phosphorylation-de...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0305400101

    authors: Toyama-Sorimachi N,Tsujimura Y,Maruya M,Onoda A,Kubota T,Koyasu S,Inaba K,Karasuyama H

    更新日期:2004-01-27 00:00:00

  • Telomere shortening is a hallmark of genetic cardiomyopathies.

    abstract::This study demonstrates that significantly shortened telomeres are a hallmark of cardiomyocytes (CMs) from individuals with end-stage hypertrophic cardiomyopathy (HCM) or dilated cardiomyopathy (DCM) as a result of heritable defects in cardiac proteins critical to contractile function. Positioned at the ends of chromo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1714538115

    authors: Chang ACY,Chang ACH,Kirillova A,Sasagawa K,Su W,Weber G,Lin J,Termglinchan V,Karakikes I,Seeger T,Dainis AM,Hinson JT,Seidman J,Seidman CE,Day JW,Ashley E,Wu JC,Blau HM

    更新日期:2018-09-11 00:00:00

  • Multiple forms of tubulin in the cilia and cytoplasm of Tetrahymena thermophila.

    abstract::Most higher eukaryotic tubulins are separated into alpha- and beta-tubulin when electrophoresed in NaDodSO4- denaturing gels, while many lower eukaryotic tubulins are poorly resolved under these conditions, which include a stacking gel (pH 6.80) and a separating gel (pH 8.80). By lowering the pH of the separating gel ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.20.6908

    authors: Suprenant KA,Hays E,LeCluyse E,Dentler WL

    更新日期:1985-10-01 00:00:00

  • Association of INAD with NORPA is essential for controlled activation and deactivation of Drosophila phototransduction in vivo.

    abstract::Visual transduction in Drosophila is a G protein-coupled phospholipase C-mediated process that leads to depolarization via activation of the transient receptor potential (TRP) calcium channel. Inactivation-no-afterpotential D (INAD) is an adaptor protein containing PDZ domains known to interact with TRP. Immunoprecipi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.23.12682

    authors: Shieh BH,Zhu MY,Lee JK,Kelly IM,Bahiraei F

    更新日期:1997-11-11 00:00:00

  • Identification of tumor-initiating cells in a highly aggressive brain tumor using promoter activity of nucleostemin.

    abstract::Controversy remains over whether the cancer stem cell (CSC) theory applies to all tumors. To determine whether cells within a highly aggressive solid tumor are stochastically or hierarchically organized, we combined a reporter system where the nucleostemin (NS) promoter drives GFP expression (termed NS-GFP) with a mou...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0905016106

    authors: Tamase A,Muraguchi T,Naka K,Tanaka S,Kinoshita M,Hoshii T,Ohmura M,Shugo H,Ooshio T,Nakada M,Sawamoto K,Onodera M,Matsumoto K,Oshima M,Asano M,Saya H,Okano H,Suda T,Hamada J,Hirao A

    更新日期:2009-10-06 00:00:00