Abstract:
:Clones of Friend erythroleukemia cells, characterized by the presence of 40-70% benzidine-positive cells synthesizing hemoglobin in the absence of inducing drugs, were treated with several phorbol diesters with a known range of tumor-promoting activity on mouse skin. Good correlation was found between the reported tumor-promoting activity of a particular phorbol diester and its ability to inhibit spontaneous erythroid differentiation in culture. The inhibition of differentiation by 12-O-tetradecanoyl-phorbol-13-acetate, the most active tumor promoter, was maximum after 4 days of treatment; this inhibition was reversed by removal of the phorbol diester no matter how long the period of treatment. Unlike control cells, which gradually revert to a population with a low percentage of benzidine-positive cells, cells treated with 12-O-tetradecanoyl-phorbol-13-acetate retained a high potential for spontaneous differentiation.
journal_name
Proc Natl Acad Sci U S Aauthors
Rovera G,O'Brien TG,Diamond Ldoi
10.1073/pnas.74.7.2894subject
Has Abstractpub_date
1977-07-01 00:00:00pages
2894-8issue
7eissn
0027-8424issn
1091-6490journal_volume
74pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2002-10-29 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2017-06-27 00:00:00