An intron within the 16S ribosomal RNA gene of the archaeon Pyrobaculum aerophilum.

abstract：:The designed assembly of proteins into well-defined supramolecular architectures not only tests our understanding of protein-protein interactions, but it also provides an opportunity to tailor materials with new physical and chemical properties. Previously, we described that RIDC3, a designed variant of the monomeric ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brodin JD,Carr JR,Sontz PA,Tezcan FA

更新日期：2014-02-25 00:00:00

abstract：:Misfolded proteins are an emerging hallmark of cardiac diseases. Although some misfolded proteins, such as desmin, are associated with mutations in the genes encoding these disease-associated proteins, little is known regarding more general mechanisms that contribute to the generation of misfolded proteins in the hear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Y,Satz JS,Vo MN,Nangle LA,Schimmel P,Ackerman SL

更新日期：2014-12-09 00:00:00

abstract：:We propose a model for DNA polymerase fidelity in which free energy differences, delta delta G, between matched and mismatched nucleotides are magnified at the enzyme's active site. Both hydrogen bonding and stacking components of the interaction energy are amplified, with the most profound effect being on the magnitu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petruska J,Sowers LC,Goodman MF

更新日期：1986-03-01 00:00:00

abstract：:Inhibition of protein synthesis in rabbit reticulocyte lysates by double-stranded RNA is caused by the inactivation of IF-3, an initiation factor required for the recycling of ribosomes and for their binding to messenger RNA. The evidence for this is that (i) the inhibition can be overcome by addition of exogenous If-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaempfer R,Kaufman J

更新日期：1973-04-01 00:00:00

abstract：:Conjugative DNA transfer of the self-transmissible broad-host-range plasmid RP4 is initiated by strand- and site-specific cleavage at the nick site (nic) of the transfer origin (oriT). Cleavage results in covalent attachment of the plasmid-encoded relaxase (TraI) to the 5'-terminal 2'-deoxycytidine residue at nic. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pansegrau W,Schröder W,Lanka E

更新日期：1993-04-01 00:00:00

abstract：:The crystal structure of fructose-1,6-bisphosphatase (EC 3.1.3.11) complexed with fructose 6-phosphate, AMP, and Mg2+ has been solved by the molecular replacement method and refined at 2.5-A resolution to a R factor of 0.215, with root-mean-square deviations of 0.013 A and 3.5 degrees for bond lengths and bond angles,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ke HM,Zhang YP,Lipscomb WN

更新日期：1990-07-01 00:00:00

abstract：:We recently have shown that mice deficient for the 86-kDa component (Ku80) of the DNA-dependent protein kinase exhibit growth retardation and a profound deficiency in V(D)J (variable, diversity, and joining) recombination. These defects may be related to abnormalities in DNA metabolism that arise from the inability of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nussenzweig A,Sokol K,Burgman P,Li L,Li GC

更新日期：1997-12-09 00:00:00

abstract：:Polysomes (S-20) from homogenates of guinea pig liver synthesized serum albumin and a precursor of the fourth component of guinea pig complement (C4) in vitro. The C4 precursor (pro-C4) accounted for approximately 0.2% and albumin 4% of the radiolabeled protein precipitable by trichloroacetic acid and not bound to pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall RE,Colten HR

更新日期：1977-04-01 00:00:00

abstract：:Epidermal growth factor (EGF) receptor monomers and noncovalently associated dimers were isolated by sucrose density gradient centrifugation, and their respective binding and autophosphorylation activities were determined. We find that monomers are low-affinity receptors and dimers are high-affinity receptors. In the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Böni-Schnetzler M,Pilch PF

更新日期：1987-11-01 00:00:00

abstract：:During protein synthesis, elongation factor G (EF-G) binds to the ribosome and promotes the step of translocation, a process in which tRNA moves from the A to the P site of the ribosome and the mRNA is advanced by one codon. By using three-dimensional cryo-electron microscopy, we have visualized EF-G in a ribosome-EF-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agrawal RK,Penczek P,Grassucci RA,Frank J

更新日期：1998-05-26 00:00:00

abstract：:Two types of progenitor cells of the human granulocytic and monocytic lineages (CFU-GM) can be distinguished by using mouse monoclonal antibodies against human hemopoietic cells. Type 1 CFU-GM contribute all of the peripheral blood CFU-GM as well as a small fraction of bone marrow CFU-GM and express surface antigens r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrero D,Broxmeyer HE,Pagliardi GL,Venuta S,Lange B,Pessano S,Rovera G

更新日期：1983-07-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the ongoing coronavirus disease 2019 (COVID-19) pandemic that is a serious global health problem. Evasion of IFN-mediated antiviral signaling is a common defense strategy that pathogenic viruses use to replicate and propagate in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miorin L,Kehrer T,Sanchez-Aparicio MT,Zhang K,Cohen P,Patel RS,Cupic A,Makio T,Mei M,Moreno E,Danziger O,White KM,Rathnasinghe R,Uccellini M,Gao S,Aydillo T,Mena I,Yin X,Martin-Sancho L,Krogan NJ,Chanda SK,Schot

更新日期：2020-11-10 00:00:00

abstract：:Regional cerebral blood flow was measured with positron-emission tomography during two encoding and two retrieval tasks that were designed to compare memory for object features with memory for object locations. Bilateral increases in regional cerebral blood flow were observed in both anterior and posterior regions of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Owen AM,Milner B,Petrides M,Evans AC

更新日期：1996-08-20 00:00:00

abstract：:Horizontal cells of the carp retina possess dopamine receptors linked to adenylate cyclase. Isolated, intact horizontal cells respond to micromolar concentrations of dopamine, whereas nanomolar concentrations of haloperidol, (+)-butaclamol, and flupenthixol block the dopamine response. Preincubation in Ringer's soluti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Buskirk R,Dowling JE

更新日期：1982-05-01 00:00:00

abstract：:The human epidermal growth factor receptor (EGFR) is a key representative of tyrosine kinase receptors, ubiquitous actors in cell signaling, proliferation, differentiation, and migration. Although the receptor is well-studied, a central issue remains: How does the compositional diversity and functional diversity of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coskun Ü,Grzybek M,Drechsel D,Simons K

更新日期：2011-05-31 00:00:00

abstract：:Y chromosomes control essential male functions in many species, including sex determination and fertility. However, because of obstacles posed by repeat-rich heterochromatin, knowledge of Y chromosome sequences is limited to a handful of model organisms, constraining our understanding of Y biology across the tree of l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall AB,Papathanos PA,Sharma A,Cheng C,Akbari OS,Assour L,Bergman NH,Cagnetti A,Crisanti A,Dottorini T,Fiorentini E,Galizi R,Hnath J,Jiang X,Koren S,Nolan T,Radune D,Sharakhova MV,Steele A,Timoshevskiy VA,Windbich

更新日期：2016-04-12 00:00:00

abstract：:2fTGH is a human cell line containing the selectable marker guanine phosphoribosyltransferase regulated by alpha interferon (IFN-alpha). Two IFN-alpha-unresponsive mutants were isolated previously at a low frequency (ca. 10(-8)) by selecting mutagenized 2fTGH cells in selective medium containing 6-thioguanine and IFN-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKendry R,John J,Flavell D,Müller M,Kerr IM,Stark GR

更新日期：1991-12-15 00:00:00

abstract：:The diabetogenic agent alloxan exerts a preferential cytotoxic effect on the pancreatic B cell. The determinants of such a tissue specificity were investigated. Alloxan accumulated rapidly in liver and pancreatic islets but much more slowly in muscle. The activity of glutathione peroxidase and the resistance to exogen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malaisse WJ,Malaisse-Lagae F,Sener A,Pipeleers DG

更新日期：1982-02-01 00:00:00

abstract：:Seed development in plants involves the coordinated growth of the embryo, endosperm, and maternal tissue. Several genes have been identified that influence seed size by acting maternally, such as AUXIN RESPONSE FACTOR2, APETALA2, and DA1. However, given the lack of gain-of-function effects of these genes on seed size,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adamski NM,Anastasiou E,Eriksson S,O'Neill CM,Lenhard M

更新日期：2009-11-24 00:00:00

abstract：:We report measurements of the geometrical structure and temporal evolution of metastable helical intermediates in the pathway for cholesterol crystallization in native and model biles. We find that the lecithin component in the bile can dramatically affect the kinetics along this pathway. We also present a theoretical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung DS,Benedek GB,Konikoff FM,Donovan JM

更新日期：1993-12-01 00:00:00

abstract：:Human T-cell leukemia virus type I (HTLV-I) is a causative agent of adult T-cell leukemia (ATL). To elucidate the role of HTLV-I in leukemogenesis, we examined the biological activity of a defective HTLV-I provirus with the env-pX 3' long terminal repeat region cloned from leukemic cells of an ATL patient. Transfectio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanaka A,Takahashi C,Yamaoka S,Nosaka T,Maki M,Hatanaka M

更新日期：1990-02-01 00:00:00

abstract：:Proteins that recognize the core sequence GATA are important regulators of hematopoietic-specific gene transcription. We have characterized cDNAs encoding the Xenopus laevis homologues of three related transcription factors, designated GATA-1, -2, and -3. Comparative sequence analysis reveals strong conservation of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zon LI,Mather C,Burgess S,Bolce ME,Harland RM,Orkin SH

更新日期：1991-12-01 00:00:00

abstract：:The Hyal-1 locus, which we have previously described and mapped to mouse chromosome 9, influences the serum levels and molecular weight forms of hyaluronidase. We have also shown that the growth of two transplantable tumors, the 3LL carcinoma and the B16F10 melanoma, is influenced by the alleles at Hyal-1, in that the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Maeyer-Guignard J,Lauret E,Eusèbe L,De Maeyer E

更新日期：1993-06-15 00:00:00

abstract：:The synthetic polypeptide antigen poly(LTyr, LGlu)-poly(DLAl)- -poly(LLys)[T,G)-A- -L] was covalently linked to N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), which is the minimal adjuvant-active structure that can substitute for Mycobacteria in complete Freund's adjuvant. When injected in aqueous solution into mice, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mozes E,Sela M,Chedid L

更新日期：1980-08-01 00:00:00

abstract：:An expression for the equilibrium of the mean phenotypic value of a quantitative character is derived for a model in which the fitness of an individual depends on its own phenotype and the mean phenotypic value of a group of related individuals. When selection is weak the equilibrium mean is well predicted by Hamilton...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyd R,Richerson PJ

更新日期：1980-12-01 00:00:00

abstract：:Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sumida-Yasumoto C,Yudelevich A,Hurwitz J

更新日期：1976-06-01 00:00:00

abstract：:Current drug discovery efforts focus on identifying lead compounds acting on a molecular target associated with an established pathological state. Concerted molecular changes that occur in specific cell types during disease progression have generally not been identified. Here, we used constellation pharmacology to inv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raghuraman S,Xie JY,Giacobassi MJ,Tun JO,Chase K,Lu D,Teichert RW,Porreca F,Olivera BM

更新日期：2020-10-20 00:00:00

abstract：:The preservation of our cultural heritage is of great importance to future generations. Despite this, significant problems have arisen with the conservation of waterlogged wooden artifacts. Three major issues facing conservators are structural instability on drying, biological degradation, and chemical degradation on ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walsh Z,Janeček ER,Hodgkinson JT,Sedlmair J,Koutsioubas A,Spring DR,Welch M,Hirschmugl CJ,Toprakcioglu C,Nitschke JR,Jones M,Scherman OA

更新日期：2014-12-16 00:00:00

abstract：:Wood-degrading brown rot fungi are essential recyclers of plant biomass in forest ecosystems. Their efficient cellulolytic systems, which have potential biotechnological applications, apparently depend on a combination of two mechanisms: lignocellulose oxidation (LOX) by reactive oxygen species (ROS) and polysaccharid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang J,Presley GN,Hammel KE,Ryu JS,Menke JR,Figueroa M,Hu D,Orr G,Schilling JS

更新日期：2016-09-27 00:00:00

abstract：:Plants sense phosphate (Pi) deficiency and initiate signaling that controls adaptive responses necessary for Pi acquisition. Herein, evidence establishes that AtSIZ1 is a plant small ubiquitin-like modifier (SUMO) E3 ligase and is a focal controller of Pi starvation-dependent responses. T-DNA insertional mutated allel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miura K,Rus A,Sharkhuu A,Yokoi S,Karthikeyan AS,Raghothama KG,Baek D,Koo YD,Jin JB,Bressan RA,Yun DJ,Hasegawa PM

更新日期：2005-05-24 00:00:00