Abstract:
:Polysomes (S-20) from homogenates of guinea pig liver synthesized serum albumin and a precursor of the fourth component of guinea pig complement (C4) in vitro. The C4 precursor (pro-C4) accounted for approximately 0.2% and albumin 4% of the radiolabeled protein precipitable by trichloroacetic acid and not bound to polysomes. Pro-C4 is a single polypeptide chain (molecular weight 200,000) which is then converted to C4, a three-chain (molecular weights 95,000, 78,000 and 31,000) structure linked by interchain disulfide bridges. Pro-C4 was also detected intracellularly in short-term tissue cultures of guinea pig liver. C4 was found in the medium harvested from these cultures.
journal_name
Proc Natl Acad Sci U S Aauthors
Hall RE,Colten HRdoi
10.1073/pnas.74.4.1707subject
Has Abstractpub_date
1977-04-01 00:00:00pages
1707-10issue
4eissn
0027-8424issn
1091-6490journal_volume
74pub_type
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