Abstract:
:Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC phosphorylation and activated in vitro KaiC autophosphorylation. These effects of KaiA were attenuated by the kaiA2 long period mutation. Both the long period phenotype and the abnormal KaiC phosphorylation in this mutant were suppressed by a previously undocumented kaiC mutation. We propose that KaiA-stimulated circadian KaiC phosphorylation is important for circadian timing.
journal_name
Proc Natl Acad Sci U S Aauthors
Iwasaki H,Nishiwaki T,Kitayama Y,Nakajima M,Kondo Tdoi
10.1073/pnas.222467299keywords:
subject
Has Abstractpub_date
2002-11-26 00:00:00pages
15788-93issue
24eissn
0027-8424issn
1091-6490pii
222467299journal_volume
99pub_type
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