Structure, domain organization, and different conformational states of stem cell factor-induced intact KIT dimers.

abstract：:By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence from dye molecules i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhuang X,Ha T,Kim HD,Centner T,Labeit S,Chu S

更新日期：2000-12-19 00:00:00

abstract：:Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu N,Strobl-Mazzulla PH,Simoes-Costa M,Sánchez-Vásquez E,Bronner ME

更新日期：2014-12-16 00:00:00

abstract：:A compact, well-organized, and natural motif, stabilized by three disulfide bonds, is proposed as a basic scaffold for protein engineering. This motif contains 37 amino acids only and is formed by a short helix on one face and an antiparallel triple-stranded beta-sheet on the opposite face. It has been adopted by scor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vita C,Roumestand C,Toma F,Ménez A

更新日期：1995-07-03 00:00:00

abstract：:We have constructed an opal suppressor system in Escherichia coli to complement an existing amber suppressor system to study the structural basis of tRNA acceptor identity, particularly the role of middle anticodon nucleotide at position 35. The opal suppressor tRNA contains a UCA anticodon and the mRNA of the suppres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClain WH,Foss K,Jenkins RA,Schneider J

更新日期：1990-12-01 00:00:00

abstract：:The genomic concentrations of certain middle repetitive DNA sequences vary considerably among closely related species of Drosophila. In fact, the chromosomes of D. melanogaster appear to carry approximately 3 times as much middle repetitive DNA as those of the sibling species D. simulans. Although most of the middle r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dowsett AP,Young MW

更新日期：1982-08-01 00:00:00

abstract：:Gel-forming mucins, the primary macromolecular components of airway mucus, facilitate airway clearance by mucociliary transport. In cystic fibrosis (CF) altered mucus properties impair mucociliary transport. Airways primarily secrete two closely related gel-forming mucins, MUC5B and MUC5AC. However, their morphologic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ostedgaard LS,Moninger TO,McMenimen JD,Sawin NM,Parker CP,Thornell IM,Powers LS,Gansemer ND,Bouzek DC,Cook DP,Meyerholz DK,Abou Alaiwa MH,Stoltz DA,Welsh MJ

更新日期：2017-06-27 00:00:00

abstract：:14-3-3 proteins are ubiquitously expressed regulators of various cellular functions, including proliferation, metabolism, and differentiation, and altered 14-3-3 expression is associated with development and progression of cancer. We report a transforming 14-3-3 oncoprotein, which we identified through conventional cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CH,Ou WB,Mariño-Enriquez A,Zhu M,Mayeda M,Wang Y,Guo X,Brunner AL,Amant F,French CA,West RB,McAlpine JN,Gilks CB,Yaffe MB,Prentice LM,McPherson A,Jones SJ,Marra MA,Shah SP,van de Rijn M,Huntsman DG,Dal Cin P

更新日期：2012-01-17 00:00:00

abstract：:Cedar virus (CedV) is a bat-borne henipavirus related to Nipah virus (NiV) and Hendra virus (HeV), zoonotic agents of fatal human disease. CedV receptor-binding protein (G) shares only ∼30% sequence identity with those of NiV and HeV, although they can all use ephrin-B2 as an entry receptor. We demonstrate that CedV a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laing ED,Navaratnarajah CK,Cheliout Da Silva S,Petzing SR,Xu Y,Sterling SL,Marsh GA,Wang LF,Amaya M,Nikolov DB,Cattaneo R,Broder CC,Xu K

更新日期：2019-10-08 00:00:00

abstract：:Experimental intramolecular vibrational dephasing transients for several large organic molecules are reanalyzed. Fits to the experimental data, as well as full numerical quantum calculations with a factorized potential surface for all active degrees of freedom of fluorene indicate that power law decays, not exponentia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gruebele M

更新日期：1998-05-26 00:00:00

abstract：:Fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. However, mining the full-genome sequences of fungi indicates that their potential to produce secondary metabolites is greatly underestimated. Because most of the biosynthesis gene clusters are silent under laborato...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schroeckh V,Scherlach K,Nützmann HW,Shelest E,Schmidt-Heck W,Schuemann J,Martin K,Hertweck C,Brakhage AA

更新日期：2009-08-25 00:00:00

abstract：:An unprecedented, spontaneous, and complete cleavage of the triple bond of N2 in the thermal reaction of 15N2 with Ta214N+ was observed experimentally by Fourier transform ion cyclotron resonance mass spectrometry; mechanistic aspects of the degenerate ligand exchange were addressed by high-level quantum chemical calc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geng C,Li J,Weiske T,Schwarz H

更新日期：2019-10-22 00:00:00

abstract：:Incommensurate charge order in hole-doped oxides is intertwined with exotic phenomena such as colossal magnetoresistance, high-temperature superconductivity, and electronic nematicity. Here, we map, at atomic resolution, the nature of incommensurate charge-lattice order in a manganite using scanning transmission elect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El Baggari I,Savitzky BH,Admasu AS,Kim J,Cheong SW,Hovden R,Kourkoutis LF

更新日期：2018-02-13 00:00:00

abstract：:A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raderschall E,Golub EI,Haaf T

更新日期：1999-03-02 00:00:00

abstract：:Hox genes encode transcription factors widely used for diversifying animal body plans in development and evolution. To achieve functional specificity, Hox proteins associate with PBC class proteins, Pre-B cell leukemia homeobox (Pbx) in vertebrates, and Extradenticle (Exd) in Drosophila, and were thought to use a uniq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saadaoui M,Merabet S,Litim-Mecheri I,Arbeille E,Sambrani N,Damen W,Brena C,Pradel J,Graba Y

更新日期：2011-02-08 00:00:00

abstract：:Ferredoxin:NADPH oxidoreductase (FNR) is a key enzyme of photosynthetic electron transport required for generation of reduction equivalents. Recently, two proteins were found to be involved in membrane-anchoring of FNR by specific interaction via a conserved Ser/Pro-rich motif: Tic62 and Trol. Our crystallographic stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alte F,Stengel A,Benz JP,Petersen E,Soll J,Groll M,Bölter B

更新日期：2010-11-09 00:00:00

abstract：:The functional role of residue Tyr-19 of Chromatium vinosum HiPIP has been evaluated by site-directed mutagenesis experiments. The stability of the [Fe4S4] cluster prosthetic center is sensitive to side-chain replacements. Polar residues result in significant instability, while nonpolar residues (especially with aroma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal A,Li D,Cowan JA

更新日期：1995-10-10 00:00:00

abstract：:Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 90 min, and proliferating...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin K,Minami M,Lan JQ,Mao XO,Batteur S,Simon RP,Greenberg DA

更新日期：2001-04-10 00:00:00

abstract：:The highly ordered and reproducible structure of the fungal prion HET-s makes it an excellent model system for studying the inherent properties of prions, self-propagating infectious proteins that have been implicated in a number of fatal diseases. In particular, the HET-s prion-forming domain readily folds into a rel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wan W,Stubbs G

更新日期：2014-04-08 00:00:00

abstract：:We have collected a set of 347 proteins that are found in eukaryotic cells but have no significant homology to proteins in Archaea and Bacteria. We call these proteins eukaryotic signature proteins (ESPs). The dominant hypothesis for the formation of the eukaryotic cell is that it is a fusion of an archaeon with a bac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hartman H,Fedorov A

更新日期：2002-02-05 00:00:00

abstract：:The Brazilian Amazon is one of the most rapidly developing agricultural areas in the world and represents a potentially large future source of greenhouse gases from land clearing and subsequent agricultural management. In an integrated approach, we estimate the greenhouse gas dynamics of natural ecosystems and agricul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galford GL,Melillo JM,Kicklighter DW,Cronin TW,Cerri CE,Mustard JF,Cerri CC

更新日期：2010-11-16 00:00:00

abstract：:The surface density of the triggering receptors responsible for the natural killer (NK)-mediated cytotoxicity is crucial for the ability of NK cells to kill susceptible target cells. In this study, we show that transforming growth factor beta1 (TGFbeta1) down-regulates the surface expression of NKp30 and in part of NK...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castriconi R,Cantoni C,Della Chiesa M,Vitale M,Marcenaro E,Conte R,Biassoni R,Bottino C,Moretta L,Moretta A

更新日期：2003-04-01 00:00:00

abstract：:We present molecular dating analyses for land plants that incorporate 33 fossil calibrations, permit rates of molecular evolution to be uncorrelated across the tree, and take into account uncertainties in phylogenetic relationships and the fossil record. We attached a prior probability to each fossil-based minimum age...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith SA,Beaulieu JM,Donoghue MJ

更新日期：2010-03-30 00:00:00

abstract：:Steep topography, a tropical climate, and mafic lithologies contribute to efficient chemical weathering and carbon sequestration in the Southeast Asian islands. Ongoing arc-continent collision between the Sunda-Banda arc system and Australia has increased the area of subaerially exposed land in the region since the mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park Y,Maffre P,Goddéris Y,Macdonald FA,Anttila ESC,Swanson-Hysell NL

更新日期：2020-10-13 00:00:00

abstract：:Reelin is a key mediator of ordered neuronal alignment in the brain. Here, we demonstrate that Reelin molecules assemble with each other to form a huge protein complex both in vitro and in vivo. The Reelin-Reelin interaction clearly is inhibited by the function-blocking anti-Reelin antibody, CR-50, at a concentration ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Utsunomiya-Tate N,Kubo K,Tate S,Kainosho M,Katayama E,Nakajima K,Mikoshiba K

更新日期：2000-08-15 00:00:00

abstract：:Although it is now more than two decades since it was first reported that the imidazobenzodiazepine Ro15-4513 reverses behavioral alcohol effects, the molecular target(s) of Ro15-4513 and the mechanism of alcohol antagonism remain elusive. Here, we show that Ro15-4513 blocks the alcohol enhancement on recombinant "ext...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallner M,Hanchar HJ,Olsen RW

更新日期：2006-05-30 00:00:00

abstract：:We have investigated the folding dynamics of Thermus thermophilus cytochrome c(552) by time-resolved fluorescence energy transfer between the heme and each of seven site-specific fluorescent probes. We have found both an equilibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada S,Bouley Ford ND,Keller GE,Ford WC,Gray HB,Winkler JR

更新日期：2013-01-29 00:00:00

abstract：:Infants' sensitivity to contingent reactivity as an indicator of intentional agency has been demonstrated by numerous referential gaze-following studies. Here we propose that variability of signal sequences in a turn-taking exchange provides an informative cue for infants to recognize interactions that may involve com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tauzin T,Gergely G

更新日期：2019-07-30 00:00:00

abstract：:Chaperone oligomerization is often a key aspect of their function. Irrespective of whether chaperone oligomers act as reservoirs for active monomers or exhibit a chaperoning function themselves, understanding the mechanism of oligomerization will further our understanding of how chaperones maintain the proteome. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karamanos TK,Tugarinov V,Clore GM

更新日期：2020-12-01 00:00:00

abstract：:Methods to acutely manipulate protein interactions at the subcellular level are powerful tools in cell biology. Several blue-light-dependent optical dimerization tools have been developed. In these systems one protein component of the dimer (the bait) is directed to a specific subcellular location, while the other com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benedetti L,Barentine AES,Messa M,Wheeler H,Bewersdorf J,De Camilli P

更新日期：2018-03-06 00:00:00

abstract：:A cDNA clone encoding the mRNA for the highly polymorphic human enzyme phosphoglucomutase 1 (PGM1; EC 5.4.2.2) has been isolated and characterized. This was achieved indirectly by first isolating a rabbit cDNA from an expression library using anti-rabbit PGM antibodies. A comparison of the nucleotide sequences shows t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whitehouse DB,Putt W,Lovegrove JU,Morrison K,Hollyoake M,Fox MF,Hopkinson DA,Edwards YH

更新日期：1992-01-01 00:00:00