The origin of the eukaryotic cell: a genomic investigation.

abstract：:In an earlier report, evidence was presented that the down-regulation of beta-actin mRNA during myogenesis was controlled by a region 3' to the promoter of the gene. In this paper we report the location of this regulatory sequence, determined by deletion analysis and the use of chimeric genes, transfected stably into ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DePonti-Zilli L,Seiler-Tuyns A,Paterson BM

更新日期：1988-03-01 00:00:00

abstract：:Laser-polarized gases (3He and 129Xe) are currently being used in magnetic resonance imaging as strong signal sources that can be safely introduced into the lung. Recently, researchers have been investigating other tissues using 129Xe. These studies use xenon dissolved in a carrier such as lipid vesicles or blood. Sin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chawla MS,Chen XJ,Möller HE,Cofer GP,Wheeler CT,Hedlund LW,Johnson GA

更新日期：1998-09-01 00:00:00

abstract：:The envelope spike of HIV is one of the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles in infection and immune evasion, the chemical structures of the glycans on the native viral envelope glycoprotein gp120--as opposed to recombinantly ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doores KJ,Bonomelli C,Harvey DJ,Vasiljevic S,Dwek RA,Burton DR,Crispin M,Scanlan CN

更新日期：2010-08-03 00:00:00

abstract：:The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amsel AD,Rathaus M,Kronman N,Cohen HY

更新日期：2008-04-01 00:00:00

abstract：:Mobile ectothermic animals can control their body temperatures by selecting specific thermal conditions in the environment, but embryos--trapped within an immobile egg and lacking locomotor structures--have been assumed to lack that ability. Falsifying that assumption, our experimental studies show that even early sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du WG,Zhao B,Chen Y,Shine R

更新日期：2011-06-07 00:00:00

abstract：:Ca(V)1 channels initiate excitation-contraction coupling in skeletal and cardiac muscle. During the fight-or-flight response, epinephrine released by the adrenal medulla and norepinephrine released from sympathetic nerves increase muscle contractility by activation of the β-adrenergic receptor/cAMP-dependent protein k...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Emrick MA,Sadilek M,Konoki K,Catterall WA

更新日期：2010-10-26 00:00:00

abstract：:Seven morphologically nontransformed (flat) revertants with reduced tumorigenicity in vivo have been isolated from populations of Kirsten sarcoma virus-transformed NIH 3T3 cells transfected with a cDNA expression library of normal human fibroblasts. Each revertant harbors 1-10 recombinant plasmids per cell and retains...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noda M,Kitayama H,Matsuzaki T,Sugimoto Y,Okayama H,Bassin RH,Ikawa Y

更新日期：1989-01-01 00:00:00

abstract：:We have determined the packing efficiency at the protein-water interface by calculating the volumes of atoms on the protein surface and nearby water molecules in 22 crystal structures. We find that an atom on the protein surface occupies, on average, a volume approximately 7% larger than an atom of equivalent chemical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerstein M,Chothia C

更新日期：1996-09-17 00:00:00

abstract：:Leucine analogs were tested in the Krebs II ascites cell-free translation system for the ability to inhibit preprotein cleavage by replacing leucine in nascent chains of bovine preprolactin, rat preprolactin, human placental prelactogen (pre-hPL), and pre-alpha subunit of human chorionic gonadotropin (alpha-hCG). In t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hortin G,Boime I

更新日期：1980-03-01 00:00:00

abstract：:"Functional connectivity" techniques are commonplace tools for studying brain organization. A critical element of these analyses is to distinguish variance due to neurobiological signals from variance due to nonneurobiological signals. Multiecho fMRI techniques are a promising means for making such distinctions based ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Power JD,Plitt M,Gotts SJ,Kundu P,Voon V,Bandettini PA,Martin A

更新日期：2018-02-27 00:00:00

abstract：:Protein synthesis has an overall error rate of approximately 10(-4) for each mRNA codon translated. The fidelity of translation is mainly determined by two events: synthesis of cognate amino acid:tRNA pairs by aminoacyl-tRNA synthetases (aaRSs) and accurate selection of aminoacyl-tRNAs (aa-tRNAs) by the ribosome. To e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds NM,Ling J,Roy H,Banerjee R,Repasky SE,Hamel P,Ibba M

更新日期：2010-03-02 00:00:00

abstract：:Understanding the relationship between the structural and functional organization of the human brain is one of the most important goals of neuroscience. Individual variability in brain structure means that it is essential to obtain this information from the same subject. To date, this has been almost impossible. Even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walters NB,Egan GF,Kril JJ,Kean M,Waley P,Jenkinson M,Watson JD

更新日期：2003-03-04 00:00:00

abstract：:Pin1 is a modular enzyme that accelerates the cis-trans isomerization of phosphorylated-Ser/Thr-Pro (pS/T-P) motifs found in numerous signaling proteins regulating cell growth and neuronal survival. We have used NMR to investigate the interaction of Pin1 with three related ligands that include a pS-P substrate peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Namanja AT,Wang XJ,Xu B,Mercedes-Camacho AY,Wilson KA,Etzkorn FA,Peng JW

更新日期：2011-07-26 00:00:00

abstract：:We report substantially improved performance of high-power quantum cascade lasers (QCLs) by using epitaxial-side-down mounting that provides superior heat dissipation properties. We used aluminum nitride as the heatsink material and gold-tin eutectic solder. We have obtained continuous wave power output of 450 mW at 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsekoun A,Go R,Pushkarsky M,Razeghi M,Patel CK

更新日期：2006-03-28 00:00:00

abstract：:DNA mismatch repair is central to the maintenance of genomic stability. It is initiated by the recognition of base-base mismatches and insertion/deletion loops by the family of MutS proteins. Subsequently, ATP induces a unique conformational change in the MutS-mismatch complex but not in the MutS-homoduplex complex th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Yang Y,Schofield MJ,Du C,Fridman Y,Lee SD,Larson ED,Drummond JT,Alani E,Hsieh P,Erie DA

更新日期：2003-12-09 00:00:00

abstract：:Thyroid hormone receptors (TRs) are members of the nuclear hormone receptor superfamily that act as ligand-dependent transcription factors. Here we identified the ten-eleven translocation protein 3 (TET3) as a TR interacting protein increasing cell sensitivity to T3. The interaction between TET3 and TRs is independent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guan W,Guyot R,Samarut J,Flamant F,Wong J,Gauthier KC

更新日期：2017-08-01 00:00:00

abstract：:Previously it was shown that yeast ADA2 protein is necessary for the full activity of some activation domains, such as VP16 and GCN4, in vivo and in vitro. These results suggest that ADA2 protein functions as a transcriptional coactivator or adaptor that bridges the interaction between certain acidic activation domain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silverman N,Agapite J,Guarente L

更新日期：1994-11-22 00:00:00

abstract：:The respiratory burst oxidase of neutrophils can be activated in a cell-free system in which plasma membranes, cytosol, Mg2+, and a membrane-perturbing detergent, such as arachidonate or sodium dodecyl sulfate, are all required. Using the technique of preparative isoelectric focusing, the cytosol factor required for o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curnutte JT,Scott PJ,Mayo LA

更新日期：1989-02-01 00:00:00

abstract：:Cancer stem-like cells (CSCs) are a highly tumorigenic cell type present as a minority population in developmentally diverse tumors and cell lines. Using a genetic screen in an inducible model of CSC formation in a breast cell line, we identify microRNAs (miRNAs) that inhibit CSC growth and are down-regulated in CSCs....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polytarchou C,Iliopoulos D,Struhl K

更新日期：2012-09-04 00:00:00

abstract：:We describe a molecular resistance biomarker to gefitinib, epithelial membrane protein-1 (EMP-1). Gefitinib is a small-molecule inhibitor that competes for the ATP-binding site on EGF receptor (EGFR) and has been approved for patients with advanced lung cancers. Treatment with gefitinib has resulted in clinical benefi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jain A,Tindell CA,Laux I,Hunter JB,Curran J,Galkin A,Afar DE,Aronson N,Shak S,Natale RB,Agus DB

更新日期：2005-08-16 00:00:00

abstract：:Phosphatidylinositol 3 (PI3)-kinase enhancer (PIKE) is a nuclear GTPase that enhances PI3-kinase activity in a GTP-dependent manner. Both PIKE-L and -A isoforms contain GTPase, pleckstrin homology (PH), ADP ribosylation factor-GTPase-activating protein, and two ankyrin repeats domains, and C-terminal ADP ribosylation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Y,Liu Z,Ye K

更新日期：2005-11-15 00:00:00

abstract：:Recombination between unirradiated chromosomes was induced by UV or x-ray irradiation of haploids followed by a mating with heteroallelic diploids of Saccharomyces cerevisiae. The selected event of intragenic recombination did not involve the participation of the irradiated chromosome and apparently was not caused by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fabre F,Roman H

更新日期：1977-04-01 00:00:00

abstract：:Atomic level characterization of proteins and other macromolecules in the living cell is challenging. Recent advances in NMR instrumentation and methods, however, have enabled in-cell studies with prospects for multidimensional spectral characterization of individual macromolecular components. We present NMR data on t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Augustus AM,Reardon PN,Spicer LD

更新日期：2009-03-31 00:00:00

abstract：:Extreme animal movements are usually associated with a single, high-performance behavior. However, the remarkably rapid mandible strikes of the trap-jaw ant, Odontomachus bauri, can yield multiple functional outcomes. Here we investigate the biomechanics of mandible strikes in O. bauri and find that the extreme mandib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patek SN,Baio JE,Fisher BL,Suarez AV

更新日期：2006-08-22 00:00:00

abstract：:DNA and protein determinants of nucleosome positioning have been examined after in vitro reconstitutions of native or modified histone octamers onto tandem repeats of 207- and 172-base-pair DNA sequences containing the Lytechinus variegatus 5S rRNA gene and onto monomeric sequences derived from these by digestion with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong F,Hansen JC,van Holde KE

更新日期：1990-08-01 00:00:00

abstract：:We have used the newly engineered transposable element Dsg to tag a gene that gives rise to a defective kernel (dek) phenotype. Dsg requires the autonomous element Ac for transposition. Upon excision, it leaves a short DNA footprint that can create in-frame and frameshift insertions in coding sequences. Therefore, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garcia N,Li Y,Dooner HK,Messing J

更新日期：2017-05-16 00:00:00

abstract：:The mechanisms underlying analgesia related to social touch are not clear. While recent research highlights the role of the empathy of the observer to pain relief in the target, the contribution of social interaction to analgesia is unknown. The current study examines brain-to-brain coupling during pain with interpers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein P,Weissman-Fogel I,Dumas G,Shamay-Tsoory SG

更新日期：2018-03-13 00:00:00

abstract：:Iron-sulfur clusters function as cofactors of a wide range of proteins, with diverse molecular roles in both prokaryotic and eukaryotic cells. Dedicated machineries assemble the clusters and deliver them to the final acceptor molecules in a tightly regulated process. In the prototypical Gram-negative bacterium Escheri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santos JA,Alonso-García N,Macedo-Ribeiro S,Pereira PJ

更新日期：2014-06-03 00:00:00

abstract：:We present a model describing how Mg(2+) binds and stabilizes specific RNA structures. In this model, RNA stabilization arises from two energetically distinct modes of Mg(2+) binding: diffuse- and site-binding. Diffusely bound Mg(2+) are electrostatically attracted to the strong anionic field around the RNA and are ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Misra VK,Draper DE

更新日期：2001-10-23 00:00:00

abstract：:A major challenge of cancer immunotherapy is the persistence and outgrowth of subpopulations that lose expression of the target antigen. IL-15 is a potent cytokine that can promote organ-specific autoimmunity when up-regulated on tissue cells. Here we report that T cells eradicated 2-wk-old solid tumors that expressed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu RB,Engels B,Schreiber K,Ciszewski C,Schietinger A,Schreiber H,Jabri B

更新日期：2013-05-14 00:00:00