Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage.

abstract：:The protein complex formed by the Ca2+ sensor neuronal calcium sensor 1 (NCS-1) and the guanine exchange factor protein Ric8a coregulates synapse number and probability of neurotransmitter release, emerging as a potential therapeutic target for diseases affecting synapses, such as fragile X syndrome (FXS), the most co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mansilla A,Chaves-Sanjuan A,Campillo NE,Semelidou O,Martínez-González L,Infantes L,González-Rubio JM,Gil C,Conde S,Skoulakis EM,Ferrús A,Martínez A,Sánchez-Barrena MJ

更新日期：2017-02-07 00:00:00

abstract：:Saccharomyces cerevisiae strains expressing the activated RAS2Val19 gene or lacking both cAMP phosphodiesterase genes, PDE1 and PDE2, have impaired growth control and display an acute sensitivity to heat shock. We have isolated two classes of mammalian cDNAs from yeast expression libraries that suppress the heat shock...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colicelli J,Nicolette C,Birchmeier C,Rodgers L,Riggs M,Wigler M

更新日期：1991-04-01 00:00:00

abstract：:IFN-gamma treatment of cells leads to tyrosine phosphorylation of signal transducer and activator of transcription (STAT) 1 followed by dimerization through a reciprocal Src homology 2-phosphotyrosine interaction near the -COOH end of each monomer, forming a parallel structure that accumulates in the nucleus to drive ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhong M,Henriksen MA,Takeuchi K,Schaefer O,Liu B,ten Hoeve J,Ren Z,Mao X,Chen X,Shuai K,Darnell JE Jr

更新日期：2005-03-15 00:00:00

abstract：:In eukaryotes, RNA polymerase I transcription is controlled by DNA elements located within the spacers that separate the tandemly arranged rRNA genes. Unlike rRNA coding sequences, the intergenic spacers evolve rapidly and have little sequence similarity even among closely related species. Nonetheless, the arrangement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doelling JH,Gaudino RJ,Pikaard CS

更新日期：1993-08-15 00:00:00

abstract：:We present the first experimental evidence to our knowledge that ingroup relations attenuate core disgust and that this helps explain the ability of groups to coact. In study 1, 45 student participants smelled a sweaty t-shirt bearing the logo of another university, with either their student identity (ingroup conditio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reicher SD,Templeton A,Neville F,Ferrari L,Drury J

更新日期：2016-03-08 00:00:00

abstract：:Molecular landers are molecules comprising of a central rigid molecular wire maintained above a metallic surface by organic spacers, which allows specific ultrahigh vacuum-scanning tunneling microscopy experiments to be performed at the single-molecule level. The understanding of the molecule-surface interactions, int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moresco F,Gourdon A

更新日期：2005-06-21 00:00:00

abstract：:Cortical blood flow at the level of individual capillaries and the coupling of neuronal activity to flow in capillaries are fundamental aspects of homeostasis in the normal and the diseased brain. To probe the dynamics of blood flow at this level, we used two-photon laser scanning microscopy to image the motion of red...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kleinfeld D,Mitra PP,Helmchen F,Denk W

更新日期：1998-12-22 00:00:00

abstract：:By examining many alternative arrangements of cortical areas, we have found that the arrangement actually present in the brain minimizes the volume of the axons required for interconnecting the areas. Our observations support the notion that the organization of cortical areas has evolved to optimize interareal connect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klyachko VA,Stevens CF

更新日期：2003-06-24 00:00:00

abstract：:The photosynthetic apparatus converts light into chemical energy by a series of reactions that give rise to a coupled flow of electrons and protons that generate reducing power and ATP, respectively. A key intermediate in these reactions is plastoquinone (PQ), the most abundant electron and proton (hydrogen) carrier i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCauley SW,Melis A,Tang GM,Arnon DI

更新日期：1987-12-01 00:00:00

abstract：:Calcific aortic valve disease is a chronic inflammatory process, and aortic valve interstitial cells (AVICs) from diseased aortic valves express greater levels of osteogenic factors in response to proinflammatory stimulation. Here, we report that lower cellular levels of IL-37 in AVICs of diseased human aortic valves ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng Q,Song R,Fullerton DA,Ao L,Zhai Y,Li S,Ballak DB,Cleveland JC Jr,Reece TB,McKinsey TA,Xu D,Dinarello CA,Meng X

更新日期：2017-02-14 00:00:00

abstract：:The cyclic enzymatic function of a cytochrome P450, as it catalyzes the oxygen-dependent metabolism of many organic chemicals, requires the delivery of two electrons to the hemeprotein. In general these electrons are transferred from NADPH to the P450 via an FMN- and FAD-containing flavoprotein (NADPH-P450 reductase)....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faulkner KM,Shet MS,Fisher CW,Estabrook RW

更新日期：1995-08-15 00:00:00

abstract：:We have isolated a lymphoid cell line, MDS, from the pleural exudate of a patient with chronic myelomonocytic leukemia. The cells are biphenotypic, containing various T-cell and myeloid markers, and are surface negative for CD4 and CD8 but have low CD4 mRNA. The cells grow in suspension with a doubling time of 15 hr, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banerjee R,Bekesi JG,Tarcsafalvi A,Sperber K,Deak G,Choi HS,Paronetto F,Holland JF,Acs G

更新日期：1992-11-01 00:00:00

abstract：:The necroptosis cell death pathway has been implicated in host defense and in the pathology of inflammatory diseases. While phosphorylation of the necroptotic effector pseudokinase Mixed Lineage Kinase Domain-Like (MLKL) by the upstream protein kinase RIPK3 is a hallmark of pathway activation, the precise checkpoints ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petrie EJ,Birkinshaw RW,Koide A,Denbaum E,Hildebrand JM,Garnish SE,Davies KA,Sandow JJ,Samson AL,Gavin X,Fitzgibbon C,Young SN,Hennessy PJ,Smith PPC,Webb AI,Czabotar PE,Koide S,Murphy JM

更新日期：2020-04-14 00:00:00

abstract：:Helper T lymphocytes recognize fragments of foreign (or self) antigens in the peptide-binding clefts of major histocompatibility complex class II molecules; their activation is a crucial step in the induction of many immune and autoimmune responses. While studying the latter, we raised a T-cell line from the thymus of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ong B,Willcox N,Wordsworth P,Beeson D,Vincent A,Altmann D,Lanchbury JS,Harcourt GC,Bell JI,Newsom-Davis J

更新日期：1991-08-15 00:00:00

abstract：:Oreopithecus bambolii (8.3-6.7 million years old) is the latest known hominoid from Europe, dating to approximately the divergence time of the Pan-hominin lineages. Despite being the most complete nonhominin hominoid in the fossil record, the O. bambolii skeleton IGF 11778 has been, for decades, at the center of inten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hammond AS,Rook L,Anaya AD,Cioppi E,Costeur L,Moyà-Solà S,Almécija S

更新日期：2020-01-07 00:00:00

abstract：:The identification of genes contributing to the adaptation of local populations is of great biological interest. In an attempt to characterize functionally important differences among African and non-African Drosophila melanogaster populations, we surveyed neutral microsatellite variation in an 850-kb genomic sequence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harr B,Kauer M,Schlötterer C

更新日期：2002-10-01 00:00:00

abstract：:An ADP analog carrying a biotin moiety and a photoreactive group was synthesized. In the presence of vanadate ion (Vi), the analog was tightly trapped into the ATPase site of heavy meromyosin (HMM) or myosin subfragment 1 (S1) in an ADP analog/ATPase site molar ratio of 1:1. UV illumination on the HMM (or S1)-Vi-ADP a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutoh K,Yamamoto K,Wakabayashi T

更新日期：1986-01-01 00:00:00

abstract：:An unstable allele of the bronze 2 (bz2) locus was isolated from a Robertson's Mutator Zea mays line containing a family of active transposable Mu elements. This mutation is somatically unstable, resulting in numerous revertant purple sectors on a bronze kernel. By following the variegated kernel phenotype through two...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chandler VL,Walbot V

更新日期：1986-03-01 00:00:00

abstract：:In choanoflagellates, the closest living relatives of animals, multicellular rosette development is regulated by environmental bacteria. The simplicity of this evolutionarily relevant interaction provides an opportunity to identify the molecules and regulatory logic underpinning bacterial regulation of development. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woznica A,Cantley AM,Beemelmanns C,Freinkman E,Clardy J,King N

更新日期：2016-07-12 00:00:00

abstract：:The DnaE intein in Synechocystis sp. strain PCC6803 is the first and only naturally split intein that has been identified so far. It is capable of catalyzing a protein trans-splicing mechanism to assemble a mature protein from two separate precursors. Therefore, it is a powerful tool for protein modification and engin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang J,Fox GC Jr,Henry-Smith TV

更新日期：2003-03-18 00:00:00

abstract：:The signal-transducing guanine nucleotide-binding regulatory (G) proteins are heterotrimers composed of three subunits--alpha, beta, and gamma. Although multiple distinctive forms of the alpha subunit have been described, only two forms of the beta subunits of the G proteins have been identified. To investigate furthe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine MA,Smallwood PM,Moen PT Jr,Helman LJ,Ahn TG

更新日期：1990-03-01 00:00:00

abstract：:Yeast centromeric DNA (CEN DNA) binding factor 3 (CBF3) is a multisubunit protein complex that binds to the essential CDEIII element in CEN DNA. The four CBF3 proteins are required for accurate chromosome segregation and are considered to be core components of the yeast kinetochore. We have examined the structure of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pietrasanta LI,Thrower D,Hsieh W,Rao S,Stemmann O,Lechner J,Carbon J,Hansma H

更新日期：1999-03-30 00:00:00

abstract：:Pathway-specific therapy is the future of cancer management. The oncogenic phosphatidylinositol 3-kinase (PI3K) pathway is frequently activated in solid tumors; however, currently, no reliable test for PI3K pathway activation exists for human tumors. Taking advantage of the observation that loss of PTEN, the negative ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saal LH,Johansson P,Holm K,Gruvberger-Saal SK,She QB,Maurer M,Koujak S,Ferrando AA,Malmström P,Memeo L,Isola J,Bendahl PO,Rosen N,Hibshoosh H,Ringnér M,Borg A,Parsons R

更新日期：2007-05-01 00:00:00

abstract：:Mutations in the human DYNC1H1 gene are associated with neurological diseases. DYNC1H1 encodes the heavy chain of cytoplasmic dynein-1, a 1.4-MDa motor complex that traffics organelles, vesicles, and macromolecules toward microtubule minus ends. The effects of the DYNC1H1 mutations on dynein motility, and consequently...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoang HT,Schlager MA,Carter AP,Bullock SL

更新日期：2017-02-28 00:00:00

abstract：:The crystal structure of DsRed, a red fluorescent protein from a corallimorpharian, has been determined at 2.0-A resolution by multiple-wavelength anomalous dispersion and crystallographic refinement. Crystals of the selenomethionine-substituted protein have space group P2(1) and contain a tetramer with 222 noncrystal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yarbrough D,Wachter RM,Kallio K,Matz MV,Remington SJ

更新日期：2001-01-16 00:00:00

abstract：:Recent studies with fibroblasts have shown that peptidylhydroxyproline formation begins toward the end of the log phase of growth and is an expression of the activity of the enzyme, collagen proline hydroxylase, which increase in late log-phase cells. This communication reports a two- to fivefold increase in hydroxyla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Comstock JP,Udenfriend S

更新日期：1970-06-01 00:00:00

abstract：:Limited trypsinization of the fatty acid synthetase multienzyme complex from rat mammary gland results in the release of a protein, molecular weight 32,000, with thioesterase activity. The other components of the multienzyme complex--the acyl carrier protein, acetyl and malonyl transferases, condensing enzyme, keto re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith S,Agradi E,Libertini L,Dileepan KN

更新日期：1976-04-01 00:00:00

abstract：:We have constructed 26-amino acid transmembrane proteins that specifically transform cells but consist of only two different amino acids. Most proteins are long polymers of amino acids with 20 or more chemically distinct side-chains. The artificial transmembrane proteins reported here are the simplest known proteins w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heim EN,Marston JL,Federman RS,Edwards AP,Karabadzhak AG,Petti LM,Engelman DM,DiMaio D

更新日期：2015-08-25 00:00:00

abstract：:beta 1-Adrenergic receptors from turkey erythrocyte membranes have been identified by specific binding of the radiolabeled antagonist (-)-[3H]dihydroalprenolol. These receptors are inactivated by the alkylating agent N-ethylmaleimide when occupied by beta-adrenergic agonists but not when occupied by antagonists or whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vauquelin G,Bottari S,Andre C,Jacobsson B,Strosberg AD

更新日期：1980-07-01 00:00:00

abstract：:Excitation of HbCO by a single 6-psec 530-nm pulse results in photodissociation with a first-order constant of 0.89 X 10(11) sec-1. The kinetics of photodissociation, monitored by following absorbance changes in the Soret band at 440 nm, are interpreted as corresponding to predissociation followed by a corssing into a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noe LJ,Eisert WG,Rentzepis PM

更新日期：1978-02-01 00:00:00